FastQCFastQC Report
Fri 10 Feb 2017
ERR1633115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285942
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10690.3738520399241804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.3171971938365123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4450.1556259661050143No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3570.12485049415615754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2000.032.3751
CTAAGTC250.00548952529.64
AACGGCC358.8530395E-426.4285737
TTAACGG358.8530395E-426.4285735
CTCGTCC400.001927660123.12516
TAACGGC400.001927660123.12536
GTCAACC400.001927660123.12515
GTAACAC400.001927660123.1253
CTAATAT400.001927660123.1253
GTATCAA5650.021.9380531
ATCTCGC450.003818886120.55555711
TCCCTCG450.003818886120.55555713
ACTAATA450.003818886120.5555572
GCTTGCA656.878976E-519.9230772
CTTGCAC656.878976E-519.9230773
AGCTATT656.878976E-519.92307735
GTTCTAT500.007022277518.51
CTAACAC609.2136965E-418.534
TAACACA609.2136965E-418.535
TCGTCCT609.2136965E-418.517
Produced by FastQC (version 0.11.5)