##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633115.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285942
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12437137601332	33.0	33.0	33.0	27.0	33.0
2	30.815263235201545	33.0	33.0	33.0	27.0	33.0
3	31.24734386693805	33.0	33.0	33.0	27.0	33.0
4	31.398710227948325	33.0	33.0	33.0	27.0	33.0
5	31.514149722671032	33.0	33.0	33.0	27.0	33.0
6	34.15868253002357	37.0	33.0	37.0	27.0	37.0
7	34.35101873806576	37.0	37.0	37.0	27.0	37.0
8	34.53554567010093	37.0	37.0	37.0	27.0	37.0
9	34.565219520042525	37.0	37.0	37.0	27.0	37.0
10	34.65021927523764	37.0	37.0	37.0	27.0	37.0
11	34.676021710696574	37.0	37.0	37.0	27.0	37.0
12	34.61842961159955	37.0	37.0	37.0	27.0	37.0
13	34.6431409166894	37.0	37.0	37.0	27.0	37.0
14	34.61866042763917	37.0	37.0	37.0	27.0	37.0
15	34.66597421854782	37.0	37.0	37.0	27.0	37.0
16	34.65956033041666	37.0	37.0	37.0	27.0	37.0
17	34.65650726371082	37.0	37.0	37.0	27.0	37.0
18	34.630082324387466	37.0	37.0	37.0	27.0	37.0
19	34.64645277713662	37.0	37.0	37.0	27.0	37.0
20	34.52365164963524	37.0	37.0	37.0	27.0	37.0
21	34.60501430360003	37.0	37.0	37.0	27.0	37.0
22	34.64205328353302	37.0	37.0	37.0	27.0	37.0
23	34.61587314910017	37.0	37.0	37.0	27.0	37.0
24	34.5838736526988	37.0	37.0	37.0	27.0	37.0
25	34.60369235719132	37.0	37.0	37.0	27.0	37.0
26	34.50966629596212	37.0	37.0	37.0	27.0	37.0
27	34.44388722188416	37.0	37.0	37.0	27.0	37.0
28	34.43953668925866	37.0	37.0	37.0	27.0	37.0
29	34.48946289807024	37.0	37.0	37.0	27.0	37.0
30	34.46796902868414	37.0	37.0	37.0	27.0	37.0
31	34.45299046659812	37.0	37.0	37.0	27.0	37.0
32	34.490627469906485	37.0	37.0	37.0	27.0	37.0
33	34.449902427765075	37.0	37.0	37.0	27.0	37.0
34	34.36312259129474	37.0	37.0	37.0	27.0	37.0
35	34.384070895496286	37.0	37.0	37.0	27.0	37.0
36	34.355079002035374	37.0	37.0	37.0	27.0	37.0
37	34.377167397584124	37.0	37.0	37.0	27.0	37.0
38	34.24164690741479	37.0	37.0	37.0	27.0	37.0
39	34.283938700855416	37.0	37.0	37.0	27.0	37.0
40	34.34227920347483	37.0	37.0	37.0	27.0	37.0
41	34.35912527715411	37.0	37.0	37.0	27.0	37.0
42	34.19183610662302	37.0	37.0	37.0	27.0	37.0
43	33.95985549517035	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	16.0
17	17.0
18	8.0
19	17.0
20	40.0
21	144.0
22	406.0
23	802.0
24	1575.0
25	2569.0
26	3879.0
27	5441.0
28	7268.0
29	9465.0
30	11687.0
31	14703.0
32	17878.0
33	22582.0
34	31352.0
35	53374.0
36	102711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.34174762714117	21.256408642312078	13.953179316085079	24.448664414461675
2	15.78222156940918	24.34584636045072	37.668128501584235	22.20380356855586
3	18.059256772352438	27.560484294017666	31.089521651243956	23.290737282385937
4	13.263528967412972	19.028334417469278	38.98867602520791	28.71946058990984
5	13.934993809933482	37.56216295612397	35.32779374838254	13.17504948556001
6	28.46136629106602	40.589000566548464	17.89313916808304	13.05649397430248
7	25.559728896069835	33.04796077526211	23.724391659847104	17.66791866882095
8	23.888410936483623	36.35177763322632	21.235425365983314	18.524386064306746
9	25.562876387519147	15.739205852935212	20.470584943799793	38.227332815745854
10	14.397675052982773	28.865644081666915	34.40452958991684	22.33215127543348
11	32.96088017849774	24.370676570773096	24.635765295059837	18.03267795566933
12	22.131760986493767	26.27141168488715	31.0723853089088	20.52444201971029
13	27.776961761476105	22.723489378964963	26.971553671723637	22.527995187835295
14	21.756160340208854	21.758958110386022	28.526064726413047	27.958816822992077
15	23.396702827846205	29.269572150995653	25.151604171475334	22.1821208496828
16	22.239475138314763	29.177945177693378	25.555182519531932	23.027397164459927
17	21.96634282476866	28.23194913653818	26.832364605409488	22.969343433283672
18	22.345090962502887	27.835015492652353	28.148016031223115	21.67187751362164
19	24.131117499353017	26.974701163172952	28.193130075329965	20.70105126214407
20	23.989480384133845	26.563778668401284	28.12983052507152	21.316910422393352
21	22.56156842996132	27.75388015751446	27.549992655853284	22.134558756670934
22	21.872617523833505	27.694427541249624	27.723804128109897	22.709150806806974
23	22.108329661259976	27.61748886137748	28.26342405103133	22.010757426331214
24	22.7025061026362	27.619587189010357	27.306236929167454	22.371669779185986
25	22.919683012638927	27.261472606332752	28.10185282329983	21.71699155772849
26	22.881563393975	27.36778787306517	27.500681956480683	22.249966776479148
27	22.68082338376314	27.39891306628617	27.52830993698023	22.391953612970465
28	21.989074707458155	27.692678934888892	28.088913136230424	22.229333221422525
29	22.958502073847146	27.60000279777018	27.306586650439602	22.13490847794308
30	22.357331207028	27.711563883584784	27.97805149296011	21.953053416427107
31	22.959551237663582	27.217407726042342	27.772065663666055	22.050975372628017
32	21.778542501626205	27.334914073483436	27.997635884200296	22.88890754069007
33	22.02089934322345	27.16425009267614	28.62153863370893	22.193311930391477
34	22.06041784697596	27.16285120758755	28.001832539466047	22.774898405970443
35	22.387057515160418	27.813682495051445	27.77626231893181	22.022997670856327
36	22.716145232249897	27.454868469829545	27.48844171195557	22.340544585964984
37	22.930524372075457	26.893915549307202	27.53600380496744	22.6395562736499
38	22.864077330367696	27.36464038161585	27.532156870973836	22.23912541704262
39	22.36887200900882	27.260423442516313	27.81158416741857	22.559120381056296
40	22.218841583258143	27.567478719460592	28.001832539466047	22.21184715781522
41	22.280742248428005	26.852998160466107	28.236845234348223	22.629414356757664
42	22.384609466255394	27.410104146994847	27.766120402039572	22.439165984710186
43	22.409789397849913	26.752977876632322	28.10604947856558	22.73118324695218
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	9.5
2	15.0
3	61.0
4	107.0
5	107.0
6	148.0
7	189.0
8	185.5
9	182.0
10	244.5
11	307.0
12	307.0
13	463.5
14	620.0
15	1231.0
16	1842.0
17	2049.5
18	2257.0
19	2257.0
20	2358.5
21	2460.0
22	2928.0
23	3396.0
24	4339.5
25	5283.0
26	5283.0
27	6352.0
28	7421.0
29	9244.5
30	11068.0
31	12148.0
32	13228.0
33	13228.0
34	14492.0
35	15756.0
36	16845.5
37	17935.0
38	18863.0
39	19791.0
40	19791.0
41	20743.0
42	21695.0
43	21310.0
44	20925.0
45	20962.5
46	21000.0
47	21000.0
48	20790.0
49	20580.0
50	20569.5
51	20559.0
52	20604.5
53	20650.0
54	20650.0
55	18552.0
56	16454.0
57	14641.0
58	12828.0
59	11070.0
60	9312.0
61	9312.0
62	8257.0
63	7202.0
64	5816.5
65	4431.0
66	3747.5
67	3064.0
68	3064.0
69	2546.0
70	2028.0
71	1729.5
72	1431.0
73	1144.5
74	858.0
75	858.0
76	663.0
77	468.0
78	350.0
79	232.0
80	179.0
81	126.0
82	126.0
83	102.0
84	78.0
85	81.5
86	85.0
87	64.0
88	43.0
89	43.0
90	32.0
91	21.0
92	15.5
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	285942.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.61734657237986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06158779116063	59.096024189514004
2	5.183116679563378	6.802047269759857
3	1.4708415324489077	2.8953815576325077
4	0.7412358696374928	1.9455172379953094
5	0.4315419309191008	1.4158318220816322
6	0.31500885360464775	1.2402027072206543
7	0.21177310099083813	0.9727192273696397
8	0.1857196054181763	0.9749142171208088
9	0.12857244408266985	0.7592924360727428
>10	1.1278422993848094	14.79192249008615
>50	0.10479752429482037	4.71884704554895
>100	0.03689300600174799	3.694032754095531
>500	5.346812464021447E-4	0.31821518738385857
>1k	5.346812464021447E-4	0.37505185811835146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1069	0.3738520399241804	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	907	0.3171971938365123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	445	0.1556259661050143	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	357	0.12485049415615754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	3.4972127214609954E-4	0.0	0.0
4	0.0	0.0	3.4972127214609954E-4	0.0	0.0
5	0.0	0.0	3.4972127214609954E-4	0.0	0.0
6	0.0	0.0	0.0017486063607304978	0.0	0.0
7	0.0	0.0	0.0034972127214609955	0.0	0.0
8	0.0	0.0	0.0034972127214609955	0.0	0.0
9	0.0	0.0	0.0038469339936070953	3.4972127214609954E-4	0.0
10	6.994425442921991E-4	0.0	0.0059452616264836925	3.4972127214609954E-4	0.0
11	6.994425442921991E-4	0.0	0.006994425442921991	3.4972127214609954E-4	0.0
12	6.994425442921991E-4	0.0	0.007693867987214191	3.4972127214609954E-4	0.0
13	6.994425442921991E-4	0.0	0.00804358925936029	3.4972127214609954E-4	0.0
14	6.994425442921991E-4	0.0	0.008393310531506389	3.4972127214609954E-4	0.0
15	6.994425442921991E-4	0.0	0.009792195620090788	3.4972127214609954E-4	0.0
16	6.994425442921991E-4	0.0	0.009792195620090788	3.4972127214609954E-4	0.0
17	6.994425442921991E-4	0.0	0.010141916892236888	3.4972127214609954E-4	0.0
18	6.994425442921991E-4	0.0	0.010141916892236888	3.4972127214609954E-4	0.0
19	6.994425442921991E-4	0.0	0.010141916892236888	6.994425442921991E-4	0.0
20	6.994425442921991E-4	0.0	0.010141916892236888	0.0010491638164382986	0.0
21	6.994425442921991E-4	0.0	0.010141916892236888	0.0010491638164382986	0.0
22	6.994425442921991E-4	0.0	0.010141916892236888	0.0013988850885843982	0.0
23	6.994425442921991E-4	0.0	0.010141916892236888	0.0013988850885843982	0.0
24	6.994425442921991E-4	0.0	0.010141916892236888	0.0017486063607304978	0.0
25	6.994425442921991E-4	0.0	0.010141916892236888	0.0017486063607304978	0.0
26	6.994425442921991E-4	0.0	0.010141916892236888	0.002448048905022697	0.0
27	6.994425442921991E-4	0.0	0.010141916892236888	0.004196655265753194	0.0
28	6.994425442921991E-4	0.0	0.010141916892236888	0.012240244525113485	0.0
29	6.994425442921991E-4	0.0	0.010141916892236888	0.024830210322373068	0.0
30	6.994425442921991E-4	0.0	0.010141916892236888	0.046862650467577344	0.0
31	6.994425442921991E-4	0.0	0.010141916892236888	0.10246833273880718	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	200	0.0	32.375	1
CTAAGTC	25	0.005489525	29.6	4
AACGGCC	35	8.8530395E-4	26.42857	37
TTAACGG	35	8.8530395E-4	26.42857	35
CTCGTCC	40	0.0019276601	23.125	16
TAACGGC	40	0.0019276601	23.125	36
GTCAACC	40	0.0019276601	23.125	15
GTAACAC	40	0.0019276601	23.125	3
CTAATAT	40	0.0019276601	23.125	3
GTATCAA	565	0.0	21.938053	1
ATCTCGC	45	0.0038188861	20.555557	11
TCCCTCG	45	0.0038188861	20.555557	13
ACTAATA	45	0.0038188861	20.555557	2
GCTTGCA	65	6.878976E-5	19.923077	2
CTTGCAC	65	6.878976E-5	19.923077	3
AGCTATT	65	6.878976E-5	19.923077	35
GTTCTAT	50	0.0070222775	18.5	1
CTAACAC	60	9.2136965E-4	18.5	34
TAACACA	60	9.2136965E-4	18.5	35
TCGTCCT	60	9.2136965E-4	18.5	17
>>END_MODULE