FastQCFastQC Report
Fri 10 Feb 2017
ERR1633114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266847
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11670.4373292560905687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.3009215018343845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4930.18475006277005177No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2950.11055024039992956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA200.001839404237.016
GGTATCA2400.029.2916661
GTACCCT400.001927323823.1251
GCGTTTA400.001927323823.12525
GCGATAC400.001927323823.12535
GTATCAA5950.021.7647081
CGTTATA450.003818227220.55555510
ATGCGTT450.003818227220.55555536
ACCGTTA450.003818227220.5555558
GTCTTGG450.003818227220.5555551
CGATACA450.003818227220.55555536
CCGTTAT450.003818227220.5555559
AGCACCG500.00702107618.523
AGGTACT500.00702107618.523
GTACATG701.2147505E-418.51
TAGGTAC500.00702107618.522
TAGGGGT609.211498E-418.54
ACTGTGT609.211498E-418.58
CTATTAG500.00702107618.51
TAGGACC609.211498E-418.54
Produced by FastQC (version 0.11.5)