FastQCFastQC Report
Fri 10 Feb 2017
ERR1633113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243066
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13960.5743296059506471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.47517958085458273No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9280.3817893082537253No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8550.3517563131001456No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8310.34188245167979064No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7860.3233689615166251No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7540.31020381295615185No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6640.27317683262982073No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6370.26206873853192136No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6330.26042309496186217No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6320.2600116840693474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6020.2476693572939037No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5930.2439666592612706No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5920.2435552483687558No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5820.23944113944360793No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5680.23368138694840082No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5530.227510223560679No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5260.21640212946277965No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5190.21352225321517615No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5170.21269943143014655No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4870.20035710465470283No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4860.19994569376218804No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4800.1974772284070993No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4750.19542017394452538No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4740.19500876305201056No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4600.1892490105568035No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4510.1855463125241704No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT4420.18184361449153727No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4420.18184361449153727No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4350.17896373824393375No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4350.17896373824393375No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4230.17402680753375627No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4150.17073552039363793No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGC4020.16538717879094567No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT4020.16538717879094567No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA3920.16127306986579779No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT3900.1604502480807682No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT3900.1604502480807682No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC3890.1600388371882534No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3860.15880460451070902No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA3860.15880460451070902No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC3840.15798178272567945No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC3720.15304485201550194No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG3570.1468736886277801No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG3500.14399381238017656No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT3480.143170990595147No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT3470.14275957970263223No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA3460.14234816881011741No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT3450.14193675791760263No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC3430.14111393613257306No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG3420.14070252524005825No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA3370.13864547077748432No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG3330.13699982720742515No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC3280.1349427727448512No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC3260.13411995095982163No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA3240.13329712917479203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.13329712917479203No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG3210.13206289649724767No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG3140.12918302024964415No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC3140.12918302024964415No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA3110.12794878757209976No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT3100.12753737667958498No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC3090.12712596578707017No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG3030.12465750043198144No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA3020.12424608953946666No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA2970.12218903507689269No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG2940.12095480239934832No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG2940.12095480239934832No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT2920.12013198061431875No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTC2910.11972056972180396No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTT2830.11642928258168563No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC2820.11601787168917085No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2820.11601787168917085No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATT2800.11519504990414127No Hit
AAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCT2790.11478363901162647No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT2790.11478363901162647No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC2740.11272658454905252No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT2720.11190376276402293No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG2690.11066953008647858No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG2670.10984670830144899No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT2670.10984670830144899No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT2670.10984670830144899No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2670.10984670830144899No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2660.1094352974089342No Hit
AAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGAC2660.1094352974089342No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGC2640.10861247562390461No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGG2620.10778965383887504No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA2610.10737824294636024No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGT2600.10696683205384547No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT2510.10326413402121234No Hit
GGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCG2510.10326413402121234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG200.00183908237.017
CGTCCGC403.812056E-837.029
GCCGTCC403.812056E-837.027
CCGTCCG451.0740223E-732.8888928
CGCCGTC401.5932037E-632.37526
GGTATCA2550.031.9215721
CAGAAAC250.005487924429.624
CCGCTCT502.705874E-729.628
GCCGCTC502.705874E-729.627
ATCAAGG250.005487924429.610
TGTTCAG250.005487924429.622
CAGTGTC250.005487924429.626
TGGATAT250.005487924429.634
AATGTTC250.005487924429.620
AGTGTCA250.005487924429.627
TGCAATT250.005487924429.637
GCTCAAT250.005487924429.616
TCCGCCG453.9911847E-628.77777731
GCCTGGG405.9211685E-527.75000237
CCGGCAG556.227656E-726.9090916
Produced by FastQC (version 0.11.5)