FastQCFastQC Report
Fri 10 Feb 2017
ERR1633112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233357
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4690.2009796149247719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.16326915412865267No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG3660.15684123467476868No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG3450.14784214743933113No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT3270.14012864409467038No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT3120.13370072464078642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA951.0913936E-1125.315791
ATATAGC400.001926602823.1250026
GTATCAA2100.022.904761
GTATTAG603.70993E-521.5833321
GCCGTAT1001.2765668E-820.358
GTCTAGA555.12623E-420.1818181
AGTAAAG656.872046E-519.9230773
AGCCTTA759.213985E-619.7333327
AAGCCTT951.6618651E-719.4736846
GGCCGTA1052.2364475E-819.380957
AGGCCGT1052.2364475E-819.380956
TAGGCCG1052.2364475E-819.380955
CCTTAGG1153.0140654E-919.3043482
TTAGGCC1306.87578E-1018.54
GAGTAAT609.2067785E-418.51
GAATAAT609.2067785E-418.51
TCTAGAC701.21392135E-418.53
CCGTATG1103.8126018E-818.59
GCTACAC500.007018502318.53
CCGCCTC500.007018502318.533
Produced by FastQC (version 0.11.5)