##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633112.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233357
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.916946995376183	33.0	33.0	33.0	27.0	33.0
2	30.655056415706408	33.0	33.0	33.0	27.0	33.0
3	31.09590455825195	33.0	33.0	33.0	27.0	33.0
4	31.28850645148849	33.0	33.0	33.0	27.0	33.0
5	31.400266544393354	33.0	33.0	33.0	27.0	33.0
6	34.015229883826066	37.0	33.0	37.0	27.0	37.0
7	34.24654927857317	37.0	33.0	37.0	27.0	37.0
8	34.40324481374032	37.0	37.0	37.0	27.0	37.0
9	34.476985905715274	37.0	37.0	37.0	27.0	37.0
10	34.489036112051494	37.0	37.0	37.0	27.0	37.0
11	34.52989196810038	37.0	37.0	37.0	27.0	37.0
12	34.51616193214688	37.0	37.0	37.0	27.0	37.0
13	34.52603521642805	37.0	37.0	37.0	27.0	37.0
14	34.52257271048222	37.0	37.0	37.0	27.0	37.0
15	34.54423479904181	37.0	37.0	37.0	27.0	37.0
16	34.53852252128713	37.0	37.0	37.0	27.0	37.0
17	34.5172375373355	37.0	37.0	37.0	27.0	37.0
18	34.51110101689686	37.0	37.0	37.0	27.0	37.0
19	34.5316360769122	37.0	37.0	37.0	27.0	37.0
20	34.53284452576953	37.0	37.0	37.0	27.0	37.0
21	34.5418350424457	37.0	37.0	37.0	27.0	37.0
22	34.54385340915422	37.0	37.0	37.0	27.0	37.0
23	34.51995868990431	37.0	37.0	37.0	27.0	37.0
24	34.514194988793996	37.0	37.0	37.0	27.0	37.0
25	34.4894903516929	37.0	37.0	37.0	27.0	37.0
26	34.444619188625154	37.0	37.0	37.0	27.0	37.0
27	34.378128789794175	37.0	37.0	37.0	27.0	37.0
28	34.34282665615345	37.0	37.0	37.0	27.0	37.0
29	34.36268464198631	37.0	37.0	37.0	27.0	37.0
30	34.34266810080692	37.0	37.0	37.0	27.0	37.0
31	34.338734214101144	37.0	37.0	37.0	27.0	37.0
32	34.3477718688533	37.0	37.0	37.0	27.0	37.0
33	34.30894295007221	37.0	37.0	37.0	27.0	37.0
34	34.23678312628291	37.0	37.0	37.0	27.0	37.0
35	34.2234216243781	37.0	37.0	37.0	27.0	37.0
36	34.217233680583824	37.0	37.0	37.0	27.0	37.0
37	34.260977815107324	37.0	37.0	37.0	27.0	37.0
38	34.11167867259178	37.0	37.0	37.0	27.0	37.0
39	34.213934015264165	37.0	37.0	37.0	27.0	37.0
40	34.22819114061288	37.0	37.0	37.0	27.0	37.0
41	34.267607142704094	37.0	37.0	37.0	27.0	37.0
42	34.15187888085637	37.0	37.0	37.0	27.0	37.0
43	33.88334611775091	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	11.0
17	12.0
18	8.0
19	25.0
20	44.0
21	144.0
22	361.0
23	749.0
24	1331.0
25	2279.0
26	3488.0
27	4815.0
28	6415.0
29	8138.0
30	10035.0
31	12246.0
32	15150.0
33	18857.0
34	25950.0
35	43150.0
36	80144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85328488110492	21.126428605098628	12.632575838736356	25.3877106750601
2	16.528752083717222	23.60332023466191	36.14161992140797	23.72630776021289
3	18.91993812056206	25.81709569457955	29.815690122858967	25.447276061999425
4	13.793029564144208	18.027742900362963	37.777311158439645	30.401916377053183
5	14.80264144636758	36.70384860964102	35.00859198567002	13.484917958321372
6	30.863869521805647	39.520134386369385	16.55832051320509	13.057675578619882
7	27.45192987568404	31.540086648354237	22.956243009637596	18.05174046632413
8	24.891046765256668	36.12619291471865	20.502063362144696	18.480696957879985
9	25.685537609756725	15.072185535467117	20.208093179120404	39.034183675655754
10	14.341545357542307	28.71094503271811	34.34865892173794	22.598850688001644
11	34.586063413568056	23.248927608771112	24.084985665739616	18.08002331192122
12	23.026950123630318	25.219727713331935	30.610180967359025	21.143141195678723
13	28.047583745077286	22.2688841560356	26.268764168205795	23.414767930681315
14	22.109043225615686	20.504206001962658	27.094109026084496	30.292641746337157
15	23.62346104895075	29.52471963557982	23.865150820416787	22.986668495052644
16	23.41948173828083	28.28241706912585	24.738919338181415	23.55918185441191
17	22.4295821423827	27.49778236778841	26.442317993460662	23.630317496368225
18	23.49276002005511	27.197812793273823	27.31608651122529	21.993340675445776
19	24.40766722232459	26.489456069455812	26.79756767527865	22.305309032940947
20	23.60460581855269	27.35936783554811	26.914984337302933	22.12104200859627
21	22.969955904472545	27.02640160783692	27.54663455563793	22.457007932052605
22	22.863252441538073	26.67072339805534	27.358510779620925	23.10751338078566
23	23.092943430023528	26.89012971541458	27.071397044014105	22.94552981054779
24	22.975955295962837	27.47507038571802	25.93494088456742	23.614033433751718
25	23.40019797991918	27.624198117048127	26.414035147863572	22.561568755169116
26	23.67445587661823	26.677579845472817	26.877702404470405	22.770261873438553
27	23.181220190523533	26.927840176210697	26.67972248529078	23.211217147974992
28	22.867537721173996	26.484742261856297	27.615199029812693	23.032520987157017
29	23.504758803035692	26.62187121020582	26.66515253452864	23.208217452229846
30	23.28320984585849	26.962979469225264	27.01483135281993	22.738979332096314
31	23.267354311205576	27.099679889611195	27.056398565288376	22.57656723389485
32	23.028235707521095	26.957408605698564	27.040971558599058	22.973384128181284
33	23.101513989295373	26.83270696829322	27.400506520052964	22.665272522358446
34	23.645315975093954	26.475314646657267	26.80356706676894	23.075802311479833
35	23.105799268931293	27.32765676624228	27.040971558599058	22.52557240622737
36	23.841581782419212	26.34889889739755	27.229095334616062	22.580423985567176
37	23.410482651045395	26.562734351230088	26.855418950363607	23.17136404736091
38	23.67488440458182	26.715718834232526	26.89912880265002	22.710267958535635
39	23.358202239487138	26.56873374272038	26.9998328740942	23.07323114369828
40	22.80540116645312	27.556490698800552	27.21195421607237	22.426153918673965
41	23.722879536504156	26.07806922440724	27.058969733069933	23.140081506018674
42	23.164936127907026	26.871703012980113	26.92484048046555	23.03852037864731
43	23.462334534640057	26.11277998945821	27.34522641274956	23.079659063152167
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	5.5
2	7.0
3	33.0
4	59.0
5	59.0
6	63.5
7	68.0
8	73.5
9	79.0
10	101.5
11	124.0
12	124.0
13	190.5
14	257.0
15	494.0
16	731.0
17	863.0
18	995.0
19	995.0
20	1059.5
21	1124.0
22	1382.0
23	1640.0
24	2195.0
25	2750.0
26	2750.0
27	3630.5
28	4511.0
29	5465.5
30	6420.0
31	7538.5
32	8657.0
33	8657.0
34	10197.5
35	11738.0
36	13259.0
37	14780.0
38	15700.5
39	16621.0
40	16621.0
41	17161.5
42	17702.0
43	18625.0
44	19548.0
45	19543.0
46	19538.0
47	19538.0
48	18692.5
49	17847.0
50	17910.5
51	17974.0
52	17147.5
53	16321.0
54	16321.0
55	15115.5
56	13910.0
57	12200.5
58	10491.0
59	10022.5
60	9554.0
61	9554.0
62	8897.5
63	8241.0
64	6487.5
65	4734.0
66	3831.0
67	2928.0
68	2928.0
69	2311.5
70	1695.0
71	1358.0
72	1021.0
73	804.5
74	588.0
75	588.0
76	435.0
77	282.0
78	220.5
79	159.0
80	130.0
81	101.0
82	101.0
83	80.0
84	59.0
85	57.5
86	56.0
87	43.0
88	30.0
89	30.0
90	21.0
91	12.0
92	6.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	233357.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.76800880352418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.07874823464712	57.04685141065015
2	5.823137281843446	7.5430601346913235
3	1.9322897030592354	3.7545166949609907
4	0.9970097693159377	2.5829735006501706
5	0.6459836291504364	2.091953668987398
6	0.41188171370603416	1.6006055075593828
7	0.3225871611885751	1.4625329667035853
8	0.22989200427001058	1.1911717885135893
9	0.19681354602505374	1.1472499333443142
>10	1.2546799978306475	15.223940889776827
>50	0.08584570781021998	3.8946050709268243
>100	0.021131251153284916	2.4605384332354516
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	469	0.2009796149247719	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	381	0.16326915412865267	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	366	0.15684123467476868	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	345	0.14784214743933113	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	327	0.14012864409467038	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	312	0.13370072464078642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.285279635922642E-4	0.0
5	0.0	0.0	0.0	4.285279635922642E-4	0.0
6	0.0	0.0	0.0	4.285279635922642E-4	0.0
7	0.0	0.0	0.0012855838907767927	4.285279635922642E-4	0.0
8	0.0	0.0	0.002142639817961321	4.285279635922642E-4	0.0
9	0.0	0.0	0.0029996957451458497	4.285279635922642E-4	0.0
10	4.285279635922642E-4	0.0	0.004285279635922642	4.285279635922642E-4	0.0
11	4.285279635922642E-4	0.0	0.005570863526699435	4.285279635922642E-4	0.0
12	4.285279635922642E-4	0.0	0.006856447417476227	4.285279635922642E-4	0.0
13	4.285279635922642E-4	0.0	0.006856447417476227	4.285279635922642E-4	0.0
14	4.285279635922642E-4	0.0	0.007284975381068492	4.285279635922642E-4	0.0
15	4.285279635922642E-4	0.0	0.007284975381068492	4.285279635922642E-4	0.0
16	4.285279635922642E-4	0.0	0.00814203130825302	8.570559271845284E-4	0.0
17	4.285279635922642E-4	0.0	0.00814203130825302	8.570559271845284E-4	0.0
18	4.285279635922642E-4	0.0	0.00814203130825302	8.570559271845284E-4	0.0
19	4.285279635922642E-4	0.0	0.00814203130825302	8.570559271845284E-4	0.0
20	4.285279635922642E-4	0.0	0.00814203130825302	8.570559271845284E-4	0.0
21	4.285279635922642E-4	0.0	0.00814203130825302	0.0012855838907767927	0.0
22	4.285279635922642E-4	0.0	0.00814203130825302	0.0012855838907767927	0.0
23	4.285279635922642E-4	0.0	0.00814203130825302	0.0012855838907767927	0.0
24	4.285279635922642E-4	0.0	0.00814203130825302	0.002142639817961321	0.0
25	4.285279635922642E-4	0.0	0.00814203130825302	0.002142639817961321	0.0
26	4.285279635922642E-4	0.0	0.00814203130825302	0.002142639817961321	0.0
27	4.285279635922642E-4	0.0	0.00814203130825302	0.0025711677815535854	0.0
28	4.285279635922642E-4	0.0	0.00814203130825302	0.00814203130825302	0.0
29	4.285279635922642E-4	0.0	0.00814203130825302	0.019712286325244154	0.0
30	4.285279635922642E-4	0.0	0.008570559271845284	0.03171106930582755	0.0
31	4.285279635922642E-4	0.0	0.008570559271845284	0.08356295290049152	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	95	1.0913936E-11	25.31579	1
ATATAGC	40	0.0019266028	23.125002	6
GTATCAA	210	0.0	22.90476	1
GTATTAG	60	3.70993E-5	21.583332	1
GCCGTAT	100	1.2765668E-8	20.35	8
GTCTAGA	55	5.12623E-4	20.181818	1
AGTAAAG	65	6.872046E-5	19.923077	3
AGCCTTA	75	9.213985E-6	19.733332	7
AAGCCTT	95	1.6618651E-7	19.473684	6
GGCCGTA	105	2.2364475E-8	19.38095	7
AGGCCGT	105	2.2364475E-8	19.38095	6
TAGGCCG	105	2.2364475E-8	19.38095	5
CCTTAGG	115	3.0140654E-9	19.304348	2
TTAGGCC	130	6.87578E-10	18.5	4
GAGTAAT	60	9.2067785E-4	18.5	1
GAATAAT	60	9.2067785E-4	18.5	1
TCTAGAC	70	1.21392135E-4	18.5	3
CCGTATG	110	3.8126018E-8	18.5	9
GCTACAC	50	0.0070185023	18.5	3
CCGCCTC	50	0.0070185023	18.5	33
>>END_MODULE