FastQCFastQC Report
Fri 10 Feb 2017
ERR1633111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433564
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17170.39601996475722157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.28161932263748835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6700.15453312544399442No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6610.1524573073410154No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5370.1238571468110821No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4740.1093264200902289No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4700.10840383426668267No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4610.10632801616370362No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4600.10609736970781708No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4500.10379090514895148No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4420.101945733501859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.028.6619721
CTATCGC400.001929258623.12532
CGGAGGG400.001929258623.1259
TCTATCG400.001929258623.12531
GTATCAA10150.022.9655171
GTCGCCC1155.456968E-1222.5217437
GGTCGCC1303.274181E-1119.92307736
CCGTACA1500.019.73333417
AGGTCGC1254.0927262E-1019.24000235
GCTCCGA1355.4569682E-1119.18518629
TCCGAGG1306.9303496E-1018.531
CGCAATA609.2241744E-418.534
CTAATAC1900.018.53
GTCAACC1054.7802496E-717.61904715
CGAACCG1703.6379788E-1217.41176431
TCAGGGC650.001577789317.0769233
ATTATAC650.001577789317.0769233
CGATAGA650.001577789317.07692314
GCCCCGT1655.2750693E-1116.81818214
CGGCCGT1005.8608202E-616.65000213
Produced by FastQC (version 0.11.5)