##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633111.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	433564
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38093568654224	33.0	33.0	33.0	27.0	33.0
2	30.993867110737977	33.0	33.0	33.0	27.0	33.0
3	31.449343118893633	33.0	33.0	33.0	27.0	33.0
4	31.603537194047476	33.0	33.0	33.0	27.0	33.0
5	31.725507652849405	33.0	33.0	33.0	27.0	33.0
6	34.549858844369	37.0	37.0	37.0	27.0	37.0
7	34.7462658338792	37.0	37.0	37.0	27.0	37.0
8	34.93322323809172	37.0	37.0	37.0	27.0	37.0
9	35.00016606544824	37.0	37.0	37.0	27.0	37.0
10	35.021766106042016	37.0	37.0	37.0	27.0	37.0
11	35.05977894843668	37.0	37.0	37.0	33.0	37.0
12	35.01088189978873	37.0	37.0	37.0	27.0	37.0
13	35.028678580324936	37.0	37.0	37.0	27.0	37.0
14	34.9907257060088	37.0	37.0	37.0	27.0	37.0
15	35.02106494081612	37.0	37.0	37.0	27.0	37.0
16	35.020135435598895	37.0	37.0	37.0	27.0	37.0
17	34.985549999538705	37.0	37.0	37.0	27.0	37.0
18	34.98373711839544	37.0	37.0	37.0	27.0	37.0
19	34.99798876290467	37.0	37.0	37.0	27.0	37.0
20	34.9893072303051	37.0	37.0	37.0	27.0	37.0
21	34.992081907169414	37.0	37.0	37.0	27.0	37.0
22	34.99090330377983	37.0	37.0	37.0	27.0	37.0
23	34.987480510374475	37.0	37.0	37.0	27.0	37.0
24	34.97179424490963	37.0	37.0	37.0	27.0	37.0
25	34.96064018230296	37.0	37.0	37.0	27.0	37.0
26	34.91507136201345	37.0	37.0	37.0	27.0	37.0
27	34.849041894622246	37.0	37.0	37.0	27.0	37.0
28	34.826717624156984	37.0	37.0	37.0	27.0	37.0
29	34.86271692299176	37.0	37.0	37.0	27.0	37.0
30	34.81567196538458	37.0	37.0	37.0	27.0	37.0
31	34.80437028904614	37.0	37.0	37.0	27.0	37.0
32	34.84070402524195	37.0	37.0	37.0	27.0	37.0
33	34.82148886900204	37.0	37.0	37.0	27.0	37.0
34	34.74435608122445	37.0	37.0	37.0	27.0	37.0
35	34.74079951287469	37.0	37.0	37.0	27.0	37.0
36	34.71388537793728	37.0	37.0	37.0	27.0	37.0
37	34.728397652941666	37.0	37.0	37.0	27.0	37.0
38	34.59123220562593	37.0	37.0	37.0	27.0	37.0
39	34.671206557740035	37.0	37.0	37.0	27.0	37.0
40	34.687139614912674	37.0	37.0	37.0	27.0	37.0
41	34.71352326300154	37.0	37.0	37.0	27.0	37.0
42	34.599939109335644	37.0	37.0	37.0	27.0	37.0
43	34.38239106567889	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	26.0
17	14.0
18	14.0
19	21.0
20	45.0
21	144.0
22	370.0
23	821.0
24	1621.0
25	2855.0
26	4669.0
27	6650.0
28	9213.0
29	12203.0
30	15397.0
31	19176.0
32	23899.0
33	31025.0
34	42969.0
35	75289.0
36	187138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.215746694836284	21.42405734793479	13.830022787869842	24.530173169359077
2	15.767453017316935	23.726139624138533	37.84608500705778	22.660322351486748
3	17.46616416492144	27.629138950650884	30.74355804448709	24.161138839940584
4	12.391942135417148	18.71142438025297	39.79527820575509	29.101355278574793
5	13.595224695777326	37.35365482373998	35.75481359153435	13.296306888948346
6	28.527276249873147	41.04976427932208	17.59763264477678	12.825326826027991
7	25.85615964425091	32.910712143997195	23.870063012611748	17.363065199140152
8	23.8142465702872	36.306289267559116	21.057098836619275	18.822365325534406
9	25.679484459041802	15.48583369467945	20.779169857275974	38.055511989002774
10	14.776365196372392	28.407801385723907	34.25561162827172	22.56022178963198
11	33.36969859121145	24.201271323264848	24.54954747165355	17.879482613870152
12	22.864444464946352	26.12786116928527	30.66352372429445	20.344170641473923
13	27.433089463147308	22.926027068668063	26.42032087534943	23.220562592835197
14	21.576284008819922	21.509627183068705	28.23758430127963	28.676504506831748
15	23.43414121098615	29.176084730282035	25.16006864038527	22.22970541834654
16	22.35240933287819	29.044616250426696	25.730226679336845	22.872747737358267
17	21.980837892444946	27.957348857377458	26.838482899871764	23.223330350305837
18	22.37178363517266	27.73846537074111	28.303549187663187	21.58620180642304
19	24.11731601332214	26.763061508796852	27.750920279358986	21.36870219852202
20	23.621426133166036	26.899604210681698	28.093660912806413	21.38530874334585
21	22.5735992840734	27.560636953252576	27.94950687787732	21.91625688479671
22	22.146442047771494	27.522349641575406	27.7696026422858	22.5616056683673
23	22.34548993920159	27.25895138895296	28.49521639250491	21.900342279340535
24	22.60842689891227	27.74653799669714	26.928204371211635	22.716830733178952
25	22.79225212425386	27.38788275779354	28.027926672878746	21.791938445073853
26	22.758347095238534	27.78828500521261	27.699024826784512	21.754343072764343
27	22.622035039809578	27.63698093015103	27.294240296703602	22.446743733335794
28	22.032272052107647	27.35397772877822	28.274487734221477	22.339262484892657
29	23.355952062440608	27.330221143821902	27.366432637396095	21.947394156341392
30	22.863291232666917	26.993246671771644	28.205524443911393	21.937937651650046
31	22.723519480399666	27.216281794613945	27.80373831775701	22.256460407229383
32	21.65954737939497	27.145012039744998	28.086972165585706	23.10846841527433
33	21.817770848133147	27.28616767074757	28.71963539408253	22.176426087036745
34	22.490335913498352	26.88230572649021	28.33745421667851	22.289904143332933
35	22.28459927484754	27.875700011993615	27.73477502744693	22.104925685711912
36	22.950706239447925	27.47598970394221	27.388574697161204	22.184729359448664
37	22.35448515098117	26.89060899890212	27.753688036829626	23.00121781328708
38	22.597355869029716	27.078816506905557	27.66166010093089	22.66216752313384
39	22.215635984537464	27.16415569558358	28.20921478720558	22.410993532673377
40	22.083475565314465	27.720936240093735	28.2105986659409	21.984989528650903
41	22.37593527137862	26.66411417922152	28.055142954673357	22.9048075947265
42	21.844064544104217	27.58646935631187	28.076131782159035	22.49333431742488
43	22.657093301104336	26.46645016652674	28.005784613113633	22.87067191925529
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	21.0
2	29.0
3	111.0
4	193.0
5	193.0
6	234.5
7	276.0
8	272.0
9	268.0
10	359.0
11	450.0
12	450.0
13	726.0
14	1002.0
15	1742.5
16	2483.0
17	2868.5
18	3254.0
19	3254.0
20	3166.0
21	3078.0
22	3482.5
23	3887.0
24	5200.0
25	6513.0
26	6513.0
27	8305.0
28	10097.0
29	12065.5
30	14034.0
31	16577.0
32	19120.0
33	19120.0
34	21718.5
35	24317.0
36	26395.5
37	28474.0
38	30648.5
39	32823.0
40	32823.0
41	34157.0
42	35491.0
43	36250.5
44	37010.0
45	35681.0
46	34352.0
47	34352.0
48	33029.0
49	31706.0
50	31437.5
51	31169.0
52	29426.0
53	27683.0
54	27683.0
55	26142.0
56	24601.0
57	21195.5
58	17790.0
59	16174.5
60	14559.0
61	14559.0
62	12625.5
63	10692.0
64	8696.5
65	6701.0
66	5557.0
67	4413.0
68	4413.0
69	3656.5
70	2900.0
71	2368.0
72	1836.0
73	1434.0
74	1032.0
75	1032.0
76	788.0
77	544.0
78	436.0
79	328.0
80	242.5
81	157.0
82	157.0
83	134.5
84	112.0
85	104.5
86	97.0
87	72.0
88	47.0
89	47.0
90	34.5
91	22.0
92	15.5
93	9.0
94	5.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	433564.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.6746975424558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.60911928818531	56.421588701785865
2	6.39458939596914	8.143470913930601
3	1.7353958881705946	3.3150242488705217
4	0.8593729513954969	2.188812510251033
5	0.4825336389581023	1.5362591757358868
6	0.3238046807051396	1.2370899064038743
7	0.22672182172207514	1.0105510397099404
8	0.17837309925600434	0.9086282515869218
9	0.12847700100636159	0.7362660762217677
>10	0.8916145213697926	10.99577575843437
>50	0.10068534090991585	4.556300570995707
>100	0.06748836255339645	7.833863713213497
>500	0.0010944058792442667	0.43391126512328576
>1k	7.296039194961779E-4	0.682457867736731
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1717	0.39601996475722157	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1221	0.28161932263748835	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	670	0.15453312544399442	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	661	0.1524573073410154	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	537	0.1238571468110821	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	474	0.1093264200902289	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	470	0.10840383426668267	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	461	0.10632801616370362	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	460	0.10609736970781708	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	450	0.10379090514895148	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	442	0.101945733501859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.3064645588655885E-4	0.0	0.0
2	0.0	0.0	2.3064645588655885E-4	0.0	0.0
3	0.0	0.0	2.3064645588655885E-4	0.0	0.0
4	0.0	0.0	2.3064645588655885E-4	0.0	0.0
5	0.0	0.0	2.3064645588655885E-4	0.0	0.0
6	0.0	0.0	0.0018451716470924708	0.0	0.0
7	0.0	0.0	0.003229050382411824	0.0	0.0
8	0.0	0.0	0.003229050382411824	0.0	0.0
9	0.0	0.0	0.0039209897500715	2.3064645588655885E-4	0.0
10	0.0	0.0	0.004843575573617736	4.612929117731177E-4	0.0
11	0.0	0.0	0.0071500401324833245	4.612929117731177E-4	0.0
12	0.0	0.0	0.007611333044256442	4.612929117731177E-4	0.0
13	0.0	0.0	0.008533918867802677	4.612929117731177E-4	0.0
14	0.0	0.0	0.008764565323689235	4.612929117731177E-4	0.0
15	0.0	0.0	0.01014844405900859	6.919393676596765E-4	0.0
16	0.0	0.0	0.011762969250214502	9.225858235462354E-4	0.0
17	0.0	0.0	0.012224262161987619	9.225858235462354E-4	0.0
18	0.0	0.0	0.012685555073760737	0.0011532322794327943	0.0
19	0.0	0.0	0.012916201529647295	0.001383878735319353	0.0
20	0.0	0.0	0.013146847985533854	0.001383878735319353	0.0
21	0.0	0.0	0.013377494441420413	0.001383878735319353	0.0
22	0.0	0.0	0.01406943380908009	0.001383878735319353	0.0
23	0.0	0.0	0.014530726720853208	0.001383878735319353	0.0
24	0.0	0.0	0.014992019632626325	0.0018451716470924708	0.0
25	0.0	0.0	0.014992019632626325	0.0020758181029790295	0.0
26	0.0	0.0	0.014992019632626325	0.0020758181029790295	0.0
27	0.0	0.0	0.014992019632626325	0.0039209897500715	0.0
28	0.0	0.0	0.014992019632626325	0.013146847985533854	0.0
29	0.0	0.0	0.014992019632626325	0.031137271544685444	0.0
30	0.0	0.0	0.015222666088512884	0.05835355333929939	0.0
31	0.0	0.0	0.015222666088512884	0.1293926617523595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	355	0.0	28.661972	1
CTATCGC	40	0.0019292586	23.125	32
CGGAGGG	40	0.0019292586	23.125	9
TCTATCG	40	0.0019292586	23.125	31
GTATCAA	1015	0.0	22.965517	1
GTCGCCC	115	5.456968E-12	22.52174	37
GGTCGCC	130	3.274181E-11	19.923077	36
CCGTACA	150	0.0	19.733334	17
AGGTCGC	125	4.0927262E-10	19.240002	35
GCTCCGA	135	5.4569682E-11	19.185186	29
TCCGAGG	130	6.9303496E-10	18.5	31
CGCAATA	60	9.2241744E-4	18.5	34
CTAATAC	190	0.0	18.5	3
GTCAACC	105	4.7802496E-7	17.619047	15
CGAACCG	170	3.6379788E-12	17.411764	31
TCAGGGC	65	0.0015777893	17.076923	3
ATTATAC	65	0.0015777893	17.076923	3
CGATAGA	65	0.0015777893	17.076923	14
GCCCCGT	165	5.2750693E-11	16.818182	14
CGGCCGT	100	5.8608202E-6	16.650002	13
>>END_MODULE