FastQCFastQC Report
Fri 10 Feb 2017
ERR1633110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335386
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9780.2916043007161897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.1827744747842784No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4150.12373802126504983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3870.11538943187849224No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3800.11330228453185286No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3370.10048123654535371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGACG251.231762E-437.031
ACGCCGG303.5940917E-430.83333235
TGTGGCG358.8562473E-426.4285710
GTGGCGA358.8562473E-426.4285711
TGACGCC358.8562473E-426.4285733
GGTATCA2100.024.6666681
GCGAGTC451.3204834E-424.66666814
GTATCAA6150.023.1626011
CGAGTCA400.001928351623.12515
TTGACGC400.001928351623.12532
GGCGAGT400.001928351623.12513
TGTCGCC400.001928351623.12524
GGTATAT502.696576E-422.21
GTCGCCC957.0995156E-921.42105337
GCTATAC450.003820243320.5555573
CGTCTTG909.428186E-820.55555715
GACGCCG450.003820243320.55555734
CGCCGGT450.003820243320.55555736
CGCAATA759.233718E-619.73333434
TATATAC851.2399341E-619.5882363
Produced by FastQC (version 0.11.5)