##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633110.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335386
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.638249658602327	33.0	33.0	33.0	27.0	33.0
2	31.14773425247327	33.0	33.0	33.0	27.0	33.0
3	31.60882088101471	33.0	33.0	33.0	27.0	33.0
4	31.734499949312134	33.0	33.0	33.0	27.0	33.0
5	31.861559516497408	33.0	33.0	33.0	33.0	33.0
6	34.744330413314806	37.0	37.0	37.0	27.0	37.0
7	34.91868175773586	37.0	37.0	37.0	27.0	37.0
8	35.08757968430405	37.0	37.0	37.0	33.0	37.0
9	35.17093140441163	37.0	37.0	37.0	33.0	37.0
10	35.19800468713661	37.0	37.0	37.0	33.0	37.0
11	35.25386867668895	37.0	37.0	37.0	33.0	37.0
12	35.18689509997436	37.0	37.0	37.0	33.0	37.0
13	35.21898648124847	37.0	37.0	37.0	33.0	37.0
14	35.19130792579297	37.0	37.0	37.0	33.0	37.0
15	35.21376563124281	37.0	37.0	37.0	33.0	37.0
16	35.19292099252801	37.0	37.0	37.0	33.0	37.0
17	35.167457794899015	37.0	37.0	37.0	33.0	37.0
18	35.17562748594157	37.0	37.0	37.0	33.0	37.0
19	35.166587156291556	37.0	37.0	37.0	33.0	37.0
20	35.177866696880606	37.0	37.0	37.0	33.0	37.0
21	35.19100379860817	37.0	37.0	37.0	33.0	37.0
22	35.16540344558211	37.0	37.0	37.0	33.0	37.0
23	35.15716219520195	37.0	37.0	37.0	33.0	37.0
24	35.16714472279702	37.0	37.0	37.0	33.0	37.0
25	35.166476835646094	37.0	37.0	37.0	33.0	37.0
26	35.107228685753135	37.0	37.0	37.0	33.0	37.0
27	35.05155850274013	37.0	37.0	37.0	27.0	37.0
28	35.033513623108895	37.0	37.0	37.0	27.0	37.0
29	35.05633807016393	37.0	37.0	37.0	27.0	37.0
30	35.0332900001789	37.0	37.0	37.0	27.0	37.0
31	35.024780402282744	37.0	37.0	37.0	27.0	37.0
32	35.050634194629474	37.0	37.0	37.0	27.0	37.0
33	35.02362352632489	37.0	37.0	37.0	27.0	37.0
34	34.973296440519285	37.0	37.0	37.0	27.0	37.0
35	34.97026709522759	37.0	37.0	37.0	27.0	37.0
36	34.94452660516539	37.0	37.0	37.0	27.0	37.0
37	34.95865659270214	37.0	37.0	37.0	27.0	37.0
38	34.826859201039994	37.0	37.0	37.0	27.0	37.0
39	34.88837339662359	37.0	37.0	37.0	27.0	37.0
40	34.92241476984728	37.0	37.0	37.0	27.0	37.0
41	34.94464587072806	37.0	37.0	37.0	27.0	37.0
42	34.88005760526677	37.0	37.0	37.0	27.0	37.0
43	34.64065584132909	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	26.0
17	25.0
18	19.0
19	17.0
20	21.0
21	91.0
22	193.0
23	484.0
24	979.0
25	1815.0
26	2999.0
27	4445.0
28	6332.0
29	8121.0
30	10792.0
31	13634.0
32	17212.0
33	22289.0
34	31601.0
35	56839.0
36	157439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.50497039232407	20.782024294395114	13.593292504755713	25.1197128085251
2	16.01617241029739	22.79820863124877	37.8978251924648	23.28779376598904
3	17.831394274060337	26.99397112580728	29.87483079198297	25.299803808149417
4	12.73636943700691	18.016554060097917	39.016536170263514	30.230540332631655
5	13.958245126510946	37.09516795572863	35.353890740818045	13.59269617694239
6	29.76928076902435	39.769400034587015	17.3495614008933	13.111757795495341
7	26.65078446923843	32.165325922966375	23.53437531679915	17.649514290996045
8	24.3164592439756	36.37569844895136	20.595075524917558	18.712766782155487
9	25.83530618451575	15.786884366073718	20.28975568449488	38.08805376491565
10	14.82620025880627	28.290387791977007	33.92747461134335	22.955937337873376
11	34.071487778261464	23.679282975437257	24.234464169643335	18.01476507665794
12	23.08027168695175	25.454252711800727	30.40645703756269	21.059018563684827
13	27.72775250010436	22.383760800987517	26.118263731938722	23.770222966969403
14	21.894771994060573	20.87177163030061	27.61713369073247	29.61632268490635
15	23.650062912584303	28.8494451169697	24.340610520415282	23.15988145003071
16	22.91538704656724	28.711097064278174	25.14863470747139	23.224881181683195
17	22.465457711413116	27.717614927277822	26.371106724788753	23.44582063652031
18	22.929102586273725	27.5664458265998	27.544679861413417	21.959771725713058
19	24.606274561251812	26.597114966039133	26.975484963594187	21.821125509114868
20	24.055863989552336	26.54970690487975	27.32761653736292	22.066812568204995
21	23.006028874192722	27.064635971686357	27.5023405866673	22.42699456745362
22	22.730525424436323	27.0306452863268	27.350873322082613	22.887955967154262
23	22.89093760622089	26.72741259325076	27.916788416928558	22.464861383599793
24	23.078780867418438	27.42601062656163	26.280763061069933	23.214445444949998
25	23.21593626448331	26.78167842426339	27.46089580364118	22.541489507612127
26	23.127977912017794	27.353854961149242	27.053305743233167	22.464861383599793
27	23.036739756578985	27.298396474509968	26.61917909513218	23.04568467377887
28	22.58084714329161	26.99963624003387	27.60073467586602	22.818781940808503
29	23.89574997167443	26.94238876995462	26.688949449291265	22.472911809079687
30	23.408550148187462	26.649293649705115	27.17495661715158	22.76719958495584
31	23.217128920109964	26.73904098561061	27.092067051099328	22.951763043180094
32	22.442200926693424	26.603674571985714	27.48415258836087	23.46997191295999
33	22.429678042613585	26.759017967357014	27.890549993142226	22.920753996887168
34	23.020340741712534	26.434913800814584	27.624289624492377	22.920455832980505
35	22.97442350008647	27.22713530081757	27.19612625452464	22.60231494457133
36	23.577012755451925	26.982342733447428	26.836838746996	22.603805764104642
37	23.018253594365895	26.5404638237732	27.045255317753274	23.396027264107623
38	23.22547750949652	26.80821501195637	26.981448241727442	22.984859236819666
39	22.72068601551645	26.730990560130714	27.53185881342692	23.01646461092592
40	22.85336895398138	27.12635590036555	27.524106551853684	22.496168593799386
41	22.838162594741583	26.388102067468527	27.505024061827264	23.268711275962623
42	22.663140381530535	27.119199966605645	27.413785906388462	22.80387374547536
43	23.29644051928226	26.019273314926682	27.294818507630016	23.389467658161045
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	9.5
2	14.0
3	56.0
4	98.0
5	98.0
6	121.5
7	145.0
8	132.0
9	119.0
10	168.0
11	217.0
12	217.0
13	333.0
14	449.0
15	829.5
16	1210.0
17	1544.5
18	1879.0
19	1879.0
20	1878.5
21	1878.0
22	2206.5
23	2535.0
24	3403.0
25	4271.0
26	4271.0
27	5643.5
28	7016.0
29	8480.0
30	9944.0
31	11926.0
32	13908.0
33	13908.0
34	16125.5
35	18343.0
36	20133.0
37	21923.0
38	23498.0
39	25073.0
40	25073.0
41	26097.0
42	27121.0
43	27359.5
44	27598.0
45	26657.0
46	25716.0
47	25716.0
48	25059.5
49	24403.0
50	23917.5
51	23432.0
52	22292.0
53	21152.0
54	21152.0
55	20611.0
56	20070.0
57	17562.0
58	15054.0
59	13911.0
60	12768.0
61	12768.0
62	11324.0
63	9880.0
64	8256.5
65	6633.0
66	5590.5
67	4548.0
68	4548.0
69	3814.5
70	3081.0
71	2530.0
72	1979.0
73	1620.0
74	1261.0
75	1261.0
76	1002.5
77	744.0
78	575.5
79	407.0
80	310.0
81	213.0
82	213.0
83	168.5
84	124.0
85	107.0
86	90.0
87	75.0
88	60.0
89	60.0
90	40.5
91	21.0
92	12.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	335386.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.89820181692355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44508285154429	65.02838820208703
2	5.658998243884774	8.137435956408869
3	1.4661049944635847	3.1623093833022717
4	0.672581660278099	1.9342964779614509
5	0.3895289924186094	1.4003217055228032
6	0.23933118384746083	1.0324489054408783
7	0.16127376389836312	0.8116705537177573
8	0.13021537427192045	0.7489801007255018
9	0.10299767882386332	0.6664813108877518
>10	0.6255280060225581	8.708367273337922
>50	0.0720992474790369	3.5231903954664503
>100	0.03542448575552468	4.369379345689073
>500	8.335173118946984E-4	0.4767303894521544
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	978	0.2916043007161897	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	613	0.1827744747842784	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	415	0.12373802126504983	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	387	0.11538943187849224	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	380	0.11330228453185286	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	337	0.10048123654535371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.981639066627707E-4	0.0	0.0
4	0.0	0.0	2.981639066627707E-4	0.0	0.0
5	2.981639066627707E-4	0.0	2.981639066627707E-4	0.0	0.0
6	2.981639066627707E-4	0.0	5.963278133255414E-4	0.0	0.0
7	2.981639066627707E-4	0.0	0.0011926556266510827	2.981639066627707E-4	0.0
8	2.981639066627707E-4	0.0	0.001788983439976624	2.981639066627707E-4	0.0
9	2.981639066627707E-4	0.0	0.0023853112533021655	8.94491719988312E-4	0.0
10	2.981639066627707E-4	0.0	0.0029816390666277067	8.94491719988312E-4	0.0
11	2.981639066627707E-4	0.0	0.004174294693278789	8.94491719988312E-4	0.0
12	2.981639066627707E-4	0.0	0.004770622506604331	8.94491719988312E-4	0.0
13	2.981639066627707E-4	0.0	0.0059632781332554135	8.94491719988312E-4	0.0
14	2.981639066627707E-4	0.0	0.006261442039918184	8.94491719988312E-4	0.0
15	2.981639066627707E-4	0.0	0.007155933759906496	8.94491719988312E-4	0.0
16	2.981639066627707E-4	0.0	0.007454097666569267	8.94491719988312E-4	0.0
17	2.981639066627707E-4	0.0	0.007454097666569267	0.0011926556266510827	0.0
18	2.981639066627707E-4	0.0	0.007752261573232037	0.0011926556266510827	0.0
19	2.981639066627707E-4	0.0	0.008050425479894808	0.0011926556266510827	0.0
20	2.981639066627707E-4	0.0	0.008050425479894808	0.0011926556266510827	0.0
21	2.981639066627707E-4	0.0	0.008050425479894808	0.0011926556266510827	0.0
22	2.981639066627707E-4	0.0	0.008050425479894808	0.001788983439976624	0.0
23	2.981639066627707E-4	0.0	0.008050425479894808	0.0020871473466393944	0.0
24	2.981639066627707E-4	0.0	0.008348589386557578	0.0020871473466393944	0.0
25	2.981639066627707E-4	0.0	0.008348589386557578	0.0020871473466393944	0.0
26	2.981639066627707E-4	0.0	0.008348589386557578	0.003577966879953248	0.0
27	2.981639066627707E-4	0.0	0.008348589386557578	0.0038761307866160186	0.0
28	2.981639066627707E-4	0.0	0.008348589386557578	0.009541245013208662	0.0
29	2.981639066627707E-4	0.0	0.008348589386557578	0.019380653933080094	0.0
30	2.981639066627707E-4	0.0	0.008348589386557578	0.03935763567948573	0.0
31	2.981639066627707E-4	0.0	0.008348589386557578	0.09749959747872601	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGACG	25	1.231762E-4	37.0	31
ACGCCGG	30	3.5940917E-4	30.833332	35
TGTGGCG	35	8.8562473E-4	26.42857	10
GTGGCGA	35	8.8562473E-4	26.42857	11
TGACGCC	35	8.8562473E-4	26.42857	33
GGTATCA	210	0.0	24.666668	1
GCGAGTC	45	1.3204834E-4	24.666668	14
GTATCAA	615	0.0	23.162601	1
CGAGTCA	40	0.0019283516	23.125	15
TTGACGC	40	0.0019283516	23.125	32
GGCGAGT	40	0.0019283516	23.125	13
TGTCGCC	40	0.0019283516	23.125	24
GGTATAT	50	2.696576E-4	22.2	1
GTCGCCC	95	7.0995156E-9	21.421053	37
GCTATAC	45	0.0038202433	20.555557	3
CGTCTTG	90	9.428186E-8	20.555557	15
GACGCCG	45	0.0038202433	20.555557	34
CGCCGGT	45	0.0038202433	20.555557	36
CGCAATA	75	9.233718E-6	19.733334	34
TATATAC	85	1.2399341E-6	19.588236	3
>>END_MODULE