FastQCFastQC Report
Fri 10 Feb 2017
ERR1633109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441317
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11130.25219966599972354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.1692660831103266No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5900.13369074837361805No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5060.11465681131703515No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4840.10967173256412058No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4690.10627281523258791No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4620.104686653811206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4490.10174092545721103No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4450.10083454750213566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGT303.5959168E-430.83333412
GTATCAA7100.025.5352121
CCGAGTT400.001929312623.12513
TGCCGAG502.698449E-422.19999911
CGAAATT855.162474E-821.76470620
GGTATCA2250.021.3777771
TATATCG450.003822129520.5555555
CGATGTC450.003822129520.55555523
CCCGCTG450.003822129520.55555525
ACGAAAT450.003822129520.55555519
TTTCGGG555.1361945E-420.1818222
TCGTTTG701.2170382E-418.512
CCTACAT701.2170382E-418.53
CCGTGCC500.00702818518.4999989
CCTACCG500.00702818518.49999824
ACGGGGT1002.864963E-718.49999816
TTTGCCG500.00702818518.4999989
CCGAAAT500.00702818518.49999819
ATACGAA500.00702818518.49999817
TGAGCGT500.00702818518.49999831