##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633109.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441317
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.661408919212267	33.0	33.0	33.0	27.0	33.0
2	31.17515527387343	33.0	33.0	33.0	27.0	33.0
3	31.631725041183547	33.0	33.0	33.0	27.0	33.0
4	31.76602079684218	33.0	33.0	33.0	27.0	33.0
5	31.882590065644422	33.0	33.0	33.0	33.0	33.0
6	34.76047149781223	37.0	37.0	37.0	27.0	37.0
7	34.95405116956746	37.0	37.0	37.0	27.0	37.0
8	35.12236102393518	37.0	37.0	37.0	33.0	37.0
9	35.20994205978922	37.0	37.0	37.0	33.0	37.0
10	35.24870784492779	37.0	37.0	37.0	33.0	37.0
11	35.2782104473655	37.0	37.0	37.0	33.0	37.0
12	35.21071248105103	37.0	37.0	37.0	33.0	37.0
13	35.25075625910627	37.0	37.0	37.0	33.0	37.0
14	35.223397240532314	37.0	37.0	37.0	33.0	37.0
15	35.22227559781291	37.0	37.0	37.0	33.0	37.0
16	35.22804922538674	37.0	37.0	37.0	33.0	37.0
17	35.17981405656251	37.0	37.0	37.0	33.0	37.0
18	35.19858287806724	37.0	37.0	37.0	33.0	37.0
19	35.21307132967912	37.0	37.0	37.0	33.0	37.0
20	35.21515826492068	37.0	37.0	37.0	33.0	37.0
21	35.22099987084114	37.0	37.0	37.0	33.0	37.0
22	35.22506950785943	37.0	37.0	37.0	33.0	37.0
23	35.219017169064415	37.0	37.0	37.0	33.0	37.0
24	35.18615643630316	37.0	37.0	37.0	33.0	37.0
25	35.18868296485293	37.0	37.0	37.0	33.0	37.0
26	35.14637097596512	37.0	37.0	37.0	33.0	37.0
27	35.094963484298134	37.0	37.0	37.0	33.0	37.0
28	35.07417797184337	37.0	37.0	37.0	33.0	37.0
29	35.10689368413181	37.0	37.0	37.0	33.0	37.0
30	35.07152681632477	37.0	37.0	37.0	33.0	37.0
31	35.05811015664477	37.0	37.0	37.0	27.0	37.0
32	35.09148525889553	37.0	37.0	37.0	33.0	37.0
33	35.06907959584607	37.0	37.0	37.0	33.0	37.0
34	35.0300373654312	37.0	37.0	37.0	27.0	37.0
35	35.00954415986694	37.0	37.0	37.0	27.0	37.0
36	34.98856604209672	37.0	37.0	37.0	27.0	37.0
37	35.01048226105045	37.0	37.0	37.0	27.0	37.0
38	34.865128694339894	37.0	37.0	37.0	27.0	37.0
39	34.95592737193446	37.0	37.0	37.0	27.0	37.0
40	34.98356056983982	37.0	37.0	37.0	27.0	37.0
41	35.00126666319222	37.0	37.0	37.0	27.0	37.0
42	34.92044267499326	37.0	37.0	37.0	27.0	37.0
43	34.7155128852956	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	16.0
17	20.0
18	17.0
19	21.0
20	26.0
21	102.0
22	250.0
23	636.0
24	1300.0
25	2268.0
26	3755.0
27	5650.0
28	8019.0
29	10663.0
30	13823.0
31	17539.0
32	22139.0
33	29294.0
34	40998.0
35	74725.0
36	210048.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35625185524238	21.004629325405546	13.672484857823289	24.966633961528785
2	15.861387619330323	23.216644724767004	37.89067722294858	23.03129043295409
3	17.395432308295398	26.94457725399203	30.535873306489442	25.124117131223134
4	12.494873299691605	18.37500028324311	39.47230675455511	29.65781966251017
5	13.604279916703865	37.24669568586753	35.90729566275489	13.241728734673716
6	29.386359464965096	40.162513567345016	17.65533618691326	12.795790780776631
7	26.192736740257004	32.56049506363906	24.05391136076788	17.192856835336052
8	23.94899811246791	36.26010328176798	20.970413557601454	18.82048504816266
9	25.541957368512886	15.65201431170791	20.557784993553387	38.24824332622582
10	14.563001198684846	28.378013989943735	34.59576676176082	22.463218049610596
11	33.52714715272695	23.829582816886727	24.66390372453361	17.97936630585271
12	22.842537223809643	25.984269810589666	30.663899192643836	20.509293772956855
13	27.389608829934037	22.55091011676414	26.40845469356494	23.65102635973688
14	21.73539655168507	21.215135605471804	27.500866723919543	29.548601118923585
15	23.61726378091032	29.148888440735345	24.453397444467356	22.78045033388698
16	22.930909074429493	28.794947849278408	25.445428116297354	22.82871495999474
17	22.175216454385396	27.802463988470873	26.809753533174568	23.212566023969163
18	22.40543645497454	27.78660237425705	28.107686764842505	21.7002744059259
19	24.204143506821627	26.850993730130497	27.381678022827128	21.56318474022075
20	23.76024490332346	26.87342658451861	27.536668653145018	21.82965985901291
21	22.90190497986708	27.564766369752352	27.495428456189085	22.037900194191476
22	22.535954880505397	27.46007971594115	27.07011060076997	22.933854802783486
23	22.702048640772958	27.24254900672306	27.875654008343208	22.179748344160775
24	23.08340716537092	27.75193341747542	26.160786917340594	23.00387249981306
25	23.04692545267914	27.21490447909326	27.53598886967871	22.20218119854889
26	22.78407584570728	27.556155779179136	27.37759932202929	22.282169053084292
27	22.77070677086992	27.25251916422889	26.970409025711678	23.006365039189518
28	22.576968482972557	27.097075344933458	27.594223653292303	22.731732518801678
29	23.776559706514817	27.104779557551602	26.910134891699165	22.208525844234416
30	23.269441240650146	26.935286879952507	27.59535662573615	22.1999152536612
31	23.11535698828733	27.110897608748356	27.3164188100617	22.457326592902607
32	22.026570469753036	27.134236841091553	27.633424499849312	23.2057681893061
33	22.250219230167883	27.27812434145977	27.998015032278385	22.473641396093964
34	22.95039620046361	26.744947509386673	27.756691901739565	22.547964388410143
35	22.765268503139467	27.446937235592557	27.398219420507253	22.389574840760723
36	23.604574489539264	27.150551644282906	26.7578633952465	22.487010470931327
37	22.59645560900668	26.990349340723334	27.274272235150697	23.13892281511929
38	23.018601141583034	27.043372451095244	27.127892195406023	22.8101342119157
39	22.475227557515346	27.10885825834944	27.63410428331562	22.781809900819592
40	22.516014565493737	27.622321369899645	27.466650956115444	22.395013108491174
41	22.55861432938228	26.36358898478871	27.702535818923813	23.375260866905194
42	22.37235365961429	27.349501605421956	27.426090542625825	22.852054192337935
43	22.955607873705297	26.303994634242507	27.601927865910447	23.138469626141752
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	15.5
2	23.0
3	69.5
4	116.0
5	116.0
6	139.0
7	162.0
8	153.5
9	145.0
10	208.5
11	272.0
12	272.0
13	403.5
14	535.0
15	983.0
16	1431.0
17	1819.5
18	2208.0
19	2208.0
20	2356.0
21	2504.0
22	3119.0
23	3734.0
24	5128.0
25	6522.0
26	6522.0
27	8120.0
28	9718.0
29	11866.5
30	14015.0
31	16501.5
32	18988.0
33	18988.0
34	21432.0
35	23876.0
36	26053.5
37	28231.0
38	30700.0
39	33169.0
40	33169.0
41	35111.0
42	37053.0
43	37755.5
44	38458.0
45	37091.5
46	35725.0
47	35725.0
48	34298.0
49	32871.0
50	32408.5
51	31946.0
52	30289.5
53	28633.0
54	28633.0
55	26811.5
56	24990.0
57	21624.5
58	18259.0
59	16689.5
60	15120.0
61	15120.0
62	13241.5
63	11363.0
64	9544.5
65	7726.0
66	6414.0
67	5102.0
68	5102.0
69	4257.0
70	3412.0
71	2742.5
72	2073.0
73	1655.0
74	1237.0
75	1237.0
76	959.0
77	681.0
78	523.5
79	366.0
80	303.5
81	241.0
82	241.0
83	197.0
84	153.0
85	144.0
86	135.0
87	102.0
88	69.0
89	69.0
90	53.0
91	37.0
92	21.5
93	6.0
94	5.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	441317.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.82821036034284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.01576142362181	55.298727737490594
2	6.378978906714909	8.015596572705482
3	1.8783307969859546	3.54036487318032
4	0.9110969171343918	2.289703550735177
5	0.5789126650815641	1.8186023351005602
6	0.35665156544691706	1.3444667747546692
7	0.2753499660774848	1.2109821914000636
8	0.20110651617770464	1.0108130002598814
9	0.16288058279033357	0.9210145967249682
>10	1.0718467134304424	12.9537532898561
>50	0.11719767111088572	5.114657771897882
>100	0.050434911080001456	5.80744815052782
>500	0.0010885232607194559	0.4201423725779303
>1k	3.628410869064853E-4	0.2537267827885168
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.25219966599972354	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	747	0.1692660831103266	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	590	0.13369074837361805	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	506	0.11465681131703515	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	484	0.10967173256412058	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	469	0.10627281523258791	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	462	0.104686653811206	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	449	0.10174092545721103	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	445	0.10083454750213566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.531889775376883E-4	0.0	0.0
2	0.0	0.0	4.531889775376883E-4	0.0	0.0
3	0.0	0.0	4.531889775376883E-4	0.0	0.0
4	0.0	0.0	4.531889775376883E-4	0.0	0.0
5	0.0	0.0	6.797834663065324E-4	0.0	0.0
6	0.0	0.0	9.063779550753766E-4	0.0	0.0
7	0.0	0.0	0.0020393503989195975	0.0	0.0
8	0.0	0.0	0.0027191338652261298	0.0	0.0
9	0.0	0.0	0.0033989173315326624	4.531889775376883E-4	0.0
10	0.0	0.0	0.0033989173315326624	4.531889775376883E-4	0.0
11	0.0	0.0	0.004305295286608039	4.531889775376883E-4	0.0
12	0.0	0.0	0.004758484264145727	6.797834663065324E-4	0.0
13	0.0	0.0	0.0052116732416834155	6.797834663065324E-4	0.0
14	0.0	0.0	0.0056648622192211045	6.797834663065324E-4	0.0
15	0.0	0.0	0.0058914567079899486	6.797834663065324E-4	0.0
16	0.0	0.0	0.006118051196758793	6.797834663065324E-4	0.0
17	0.0	0.0	0.006118051196758793	6.797834663065324E-4	0.0
18	0.0	0.0	0.006118051196758793	6.797834663065324E-4	0.0
19	0.0	0.0	0.006118051196758793	6.797834663065324E-4	0.0
20	0.0	0.0	0.006344645685527637	6.797834663065324E-4	0.0
21	0.0	0.0	0.006571240174296481	6.797834663065324E-4	0.0
22	0.0	0.0	0.006797834663065325	6.797834663065324E-4	0.0
23	0.0	0.0	0.006797834663065325	9.063779550753766E-4	0.0
24	0.0	0.0	0.006797834663065325	0.0011329724438442208	0.0
25	0.0	0.0	0.006797834663065325	0.0013595669326130649	0.0
26	0.0	0.0	0.006797834663065325	0.0013595669326130649	0.0
27	0.0	0.0	0.006797834663065325	0.0024925393764572857	0.0
28	0.0	0.0	0.006797834663065325	0.009516968528291455	0.0
29	0.0	0.0	0.006797834663065325	0.023792421320728636	0.0
30	0.0	0.0	0.006797834663065325	0.04305295286608039	0.0
31	0.0	0.0	0.006797834663065325	0.11511000029457284	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGAGT	30	3.5959168E-4	30.833334	12
GTATCAA	710	0.0	25.535212	1
CCGAGTT	40	0.0019293126	23.125	13
TGCCGAG	50	2.698449E-4	22.199999	11
CGAAATT	85	5.162474E-8	21.764706	20
GGTATCA	225	0.0	21.377777	1
TATATCG	45	0.0038221295	20.555555	5
CGATGTC	45	0.0038221295	20.555555	23
CCCGCTG	45	0.0038221295	20.555555	25
ACGAAAT	45	0.0038221295	20.555555	19
TTTCGGG	55	5.1361945E-4	20.18182	22
TCGTTTG	70	1.2170382E-4	18.5	12
CCTACAT	70	1.2170382E-4	18.5	3
CCGTGCC	50	0.007028185	18.499998	9
CCTACCG	50	0.007028185	18.499998	24
ACGGGGT	100	2.864963E-7	18.499998	16
TTTGCCG	50	0.007028185	18.499998	9
CCGAAAT	50	0.007028185	18.499998	19
ATACGAA	50	0.007028185	18.499998	17
TGAGCGT	50	0.007028185	18.499998	31
>>END_MODULE