FastQCFastQC Report
Fri 10 Feb 2017
ERR1633108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443591
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27280.6149809171060729No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.4470334159169144No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12890.2905829919903695No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11330.25541546154002226No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10500.23670453187733745No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10040.22633461905223504No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9910.2234039915147061No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9850.22205139418969275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9830.2216005284146883No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9760.2200224982021727No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9510.21438667601461708No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9410.21213234713959478No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9200.20739825650204807No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9160.20649652495203916No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9100.2051439276270258No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8720.1965774779019412No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8650.19499944768942562No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8090.18237520598930096No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8020.18079717577678536No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT7580.17087812872668742No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7580.17087812872668742No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7520.16952553140167406No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7400.16682033675164734No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7340.16546773942663398No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7220.1627625447766073No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7180.16186081322659837No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7160.16140994745159393No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7110.16028278301408277No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6880.1550978266015316No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6840.15419609505152268No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG6820.15374522927651824No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6660.15013830307648263No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6570.14810940708896259No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6410.14450248088892698No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6320.14247358490140694No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA6280.14157185335139802No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA6230.1404446889138869No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6170.13909209158887353No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6030.13593603116384237No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5920.13345626940131788No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5890.1327799707388112No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC5850.13187823918880232No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA5810.1309765076387934No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC5740.1293984774262778No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG5730.1291730445387756No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT5480.12353722235121994No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.1233117894637177No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5470.1233117894637177No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5430.1224100579137088No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT5400.12173375925120213No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5170.11654880283865092No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT5150.11609793706364646No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG5040.11361817530112198No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5030.11339274241361975No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5020.11316730952611752No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4830.10888408466357524No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA4800.10820778600106856No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA4800.10820778600106856No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC4760.10730605445105966No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4730.10662975578855298No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA4730.10662975578855298No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG4690.10572802423854405No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG4610.10392456113852626No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC4590.1034736953635218No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT4590.1034736953635218No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT4580.10324826247601958No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA4540.10234653092601068No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG4510.101670232263504No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC4480.10099393360099732No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG4460.10054306782599286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5350.031.1214941
TTTGGCC303.595947E-430.8333329
CGACAGC250.005492749629.636
TGCATCA250.005492749629.633
ATGACAA250.005492749629.65
ACATGGG250.005492749629.630
GCGGTTA405.932033E-527.7513
GGCGTAC405.932033E-527.7520
AGCGGTT405.932033E-527.7512
ACGGCCA405.932033E-527.7525
CGTACGG405.932033E-527.7522
GAATACT358.860773E-426.42857234
CCGCTCT451.3214233E-424.66666628
CGCTCTC451.3214233E-424.66666629
GTCCGGC451.3214233E-424.66666615
GCCGTCC451.3214233E-424.66666627
GCGTACG451.3214233E-424.66666621
CAAGCAT1400.023.78571537
CCAAGCA1400.023.78571536
GTATCAA15650.023.405751
Produced by FastQC (version 0.11.5)