FastQCFastQC Report
Fri 10 Feb 2017
ERR1633107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373338
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11460.30696044870867684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.22071152682020048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4240.11357000894631675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC358.858132E-426.42857233
GCGATCT358.858132E-426.42857234
GGTATCA2150.025.8139531
GTATCAA6450.024.3798451
TGTTAGA400.001928758123.1250022
ACCTAAG502.6973695E-422.1999991
GCTATCC450.003821043520.55555513
CACGCTT450.003821043520.55555520
CCCACGA450.003821043520.55555519
GCCCACG555.134159E-420.18181818
ATATACT656.8861875E-519.9230774
TTAGTGT656.8861875E-519.9230774
TCTCCGA609.2209E-418.511
TTAGGTT500.007026205318.4999984
GGATAGT500.007026205318.4999981
TTATATC500.007026205318.4999982
GAGCGAT500.007026205318.49999832
CGAGACA500.007026205318.49999823
ATCAACG9100.017.890113
TCAACGC9300.017.5053774
Produced by FastQC (version 0.11.5)