FastQCFastQC Report
Fri 10 Feb 2017
ERR1633106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260570
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10540.4044978316767088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.3051003569098515No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA6320.24254518939248573No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG5940.22796177610622864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4420.16962812296120044No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG4140.1588824500134321No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC4090.15696357984418774No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT3990.15312583950569902No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC3870.1485205510995126No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC3850.14775300303181488No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG3650.14007752235483747No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA3470.13316958974555781No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG3470.13316958974555781No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT3450.13240204167786007No Hit
CAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAG3000.11513221015466094No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA2780.1066891814099858No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2760.10592163334228806No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT2740.10515408527459033No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG2720.10438653720689257No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG2630.10093257090225274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCTA250.005488641629.66
GGTATCA2400.024.6666681
GTATCAA5750.023.8086951
GATAGCA400.001927202923.1250022
ATAGATC502.694338E-422.26
ATTATAG705.0769795E-621.1428573
TAAGGAT450.003817991420.55555524
AGGGCCA1101.7371349E-920.1818185
GTAATAC500.007020645318.53
CGCCTCT500.007020645318.534
TATAGAT609.2107063E-418.55
ATGCCCG500.007020645318.529
TATAAGC500.007020645318.52
GTAGTGG609.2107063E-418.524
CAGGGCC1351.1386874E-917.8148144
CGCGCAC1156.348637E-817.6956534
GCGAACT752.0595457E-417.26666623
GGTAGTG752.0595457E-417.26666623
CGAACTA650.00157550517.07692324
GTTAGGT650.00157550517.0769231
Produced by FastQC (version 0.11.5)