##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633106.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260570
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.795060828184365	33.0	33.0	33.0	27.0	33.0
2	30.529032505660666	33.0	33.0	33.0	27.0	33.0
3	31.003726445868672	33.0	33.0	33.0	27.0	33.0
4	31.181701654066085	33.0	33.0	33.0	27.0	33.0
5	31.28174003146947	33.0	33.0	33.0	27.0	33.0
6	33.73421729285796	37.0	33.0	37.0	27.0	37.0
7	34.002671067275585	37.0	33.0	37.0	27.0	37.0
8	34.160858886287755	37.0	33.0	37.0	27.0	37.0
9	34.22653029895997	37.0	37.0	37.0	27.0	37.0
10	34.234685497179264	37.0	37.0	37.0	27.0	37.0
11	34.263679625436545	37.0	37.0	37.0	27.0	37.0
12	34.234673983958245	37.0	37.0	37.0	27.0	37.0
13	34.293310818590015	37.0	37.0	37.0	27.0	37.0
14	34.287442913612466	37.0	37.0	37.0	27.0	37.0
15	34.33263998157884	37.0	37.0	37.0	27.0	37.0
16	34.32947000805925	37.0	37.0	37.0	27.0	37.0
17	34.260632459607784	37.0	37.0	37.0	27.0	37.0
18	34.28748129101585	37.0	37.0	37.0	27.0	37.0
19	34.29060521165138	37.0	37.0	37.0	27.0	37.0
20	34.27616379475764	37.0	37.0	37.0	27.0	37.0
21	34.25547069885251	37.0	37.0	37.0	27.0	37.0
22	34.254304025789615	37.0	37.0	37.0	27.0	37.0
23	34.262497601412285	37.0	37.0	37.0	27.0	37.0
24	34.248098399662275	37.0	37.0	37.0	27.0	37.0
25	34.22524849368692	37.0	37.0	37.0	27.0	37.0
26	34.16169551368154	37.0	37.0	37.0	27.0	37.0
27	34.099021376213685	37.0	33.0	37.0	27.0	37.0
28	34.06216755574318	37.0	33.0	37.0	27.0	37.0
29	34.111367386882606	37.0	37.0	37.0	27.0	37.0
30	34.06438576965883	37.0	33.0	37.0	27.0	37.0
31	34.04872395133745	37.0	33.0	37.0	27.0	37.0
32	34.08857888475266	37.0	33.0	37.0	27.0	37.0
33	34.04338181678628	37.0	33.0	37.0	27.0	37.0
34	33.957182331043484	37.0	33.0	37.0	27.0	37.0
35	33.93750239858771	37.0	33.0	37.0	27.0	37.0
36	33.91844418006678	37.0	33.0	37.0	27.0	37.0
37	33.9489580534981	37.0	33.0	37.0	27.0	37.0
38	33.79701040027632	37.0	33.0	37.0	27.0	37.0
39	33.87919560962505	37.0	33.0	37.0	27.0	37.0
40	33.91018920059869	37.0	33.0	37.0	27.0	37.0
41	33.90698468741605	37.0	33.0	37.0	27.0	37.0
42	33.7834478259201	37.0	33.0	37.0	27.0	37.0
43	33.49388264190045	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	12.0
16	14.0
17	17.0
18	11.0
19	25.0
20	75.0
21	218.0
22	524.0
23	1094.0
24	1852.0
25	3101.0
26	4572.0
27	6095.0
28	8088.0
29	10165.0
30	12104.0
31	14804.0
32	17837.0
33	22397.0
34	30128.0
35	48697.0
36	78737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.46947077560732	21.248416932110374	13.072111140960201	24.210001151322103
2	16.804313620140462	24.543500786736768	35.97037264458687	22.6818129485359
3	18.809532947000807	26.828107610239094	29.739801205050465	24.622558237709637
4	13.387573396783974	19.037494723107034	38.29105422727098	29.283877652838008
5	15.576236711824079	36.2816133860383	34.198487930306634	13.943661971830986
6	29.611620677744945	39.99385961545842	17.158537053382968	13.23598265341367
7	27.3174195033964	31.896995049314963	22.77161607245654	18.013969374832097
8	24.34700848140615	35.64416471581533	20.987450589093143	19.021376213685382
9	25.968837548451475	14.949533714548874	19.94550408719346	39.13612464980619
10	15.378593084391909	28.029704110219907	33.886863414821356	22.704839390566832
11	34.42299574010823	24.020800552634608	23.593276278926968	17.9629274283302
12	23.149249721763827	25.31718923897609	30.30893809724834	21.22462294201174
13	28.204705069654988	22.249299612388228	26.20792877153932	23.33806654641747
14	22.2412403576774	21.366235560501977	27.35080784434125	29.04171623747937
15	23.82622711747323	29.201750009594353	24.606055954254135	22.365966918678282
16	23.889166059024447	28.453774417622903	24.58763480062939	23.069424722723262
17	23.248263422496834	27.247572629235904	25.683309667267913	23.82085428099935
18	23.163065586982384	26.507656291975284	27.848946540277087	22.480331580765245
19	25.24312085044326	26.418620716122348	26.973174195033966	21.36508423840043
20	24.593775185170973	26.654257972905555	26.94784510879994	21.804121733123537
21	23.016463906052117	27.27290171546993	26.90639751314426	22.804236865333692
22	23.873047549602795	26.0617108646429	27.349656522239705	22.715585063514602
23	22.501439152626933	26.591319031354338	27.886940169628122	23.020301646390607
24	23.020685420424456	27.544229957401082	26.090877691215415	23.344206930959054
25	23.38296810837779	27.21303296618951	27.379974670913764	22.024024254518938
26	24.33971677476302	26.73983958245385	26.30732624630617	22.61311739647695
27	24.01350884599148	26.338411943047934	26.213685382047053	23.434393828913535
28	22.842614268718577	26.502283455501402	27.636335725524813	23.018766550255208
29	24.076831561576544	27.20842767778332	26.706451241509	22.008289519131136
30	24.108684806385998	26.521088383159995	26.49614307095982	22.874083739494186
31	23.415205127221093	26.022565913190316	27.206892581647924	23.35533637794067
32	22.57013470468588	26.925969988870552	26.94055340215681	23.563341904286755
33	23.247879648462984	26.206393675403923	27.427562651111025	23.118164025022068
34	23.51114863568331	26.419772038223893	27.299382123805504	22.769697202287293
35	23.406762098476417	27.07756073224086	26.212917833979354	23.302759335303374
36	23.381433012242393	26.5187857389569	26.283916030241393	23.815865218559313
37	23.505392025175574	26.293510381087614	27.21264919215566	22.98844840158115
38	23.42978854050735	25.991096442414708	26.637371915416203	23.941743101661743
39	23.193383735656443	26.28276470813985	27.203822389377137	23.320029166826572
40	23.255938903173814	26.91292167171969	27.11363549142265	22.717503933683847
41	23.39524887746095	26.433204129408605	26.890279003722604	23.281267989407837
42	22.4619104271405	28.094561921940365	26.5030510035691	22.940476647350042
43	23.82392447327014	25.966918678282227	26.47848946540277	23.730667383044864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	8.5
2	9.0
3	61.5
4	114.0
5	114.0
6	141.0
7	168.0
8	185.0
9	202.0
10	261.5
11	321.0
12	321.0
13	486.0
14	651.0
15	1109.0
16	1567.0
17	1752.0
18	1937.0
19	1937.0
20	1804.0
21	1671.0
22	1918.0
23	2165.0
24	2775.5
25	3386.0
26	3386.0
27	4101.5
28	4817.0
29	5812.5
30	6808.0
31	8038.0
32	9268.0
33	9268.0
34	11759.0
35	14250.0
36	15249.0
37	16248.0
38	16848.0
39	17448.0
40	17448.0
41	17713.5
42	17979.0
43	18735.0
44	19491.0
45	19194.5
46	18898.0
47	18898.0
48	18518.5
49	18139.0
50	18689.5
51	19240.0
52	19843.0
53	20446.0
54	20446.0
55	19333.5
56	18221.0
57	15470.5
58	12720.0
59	11496.5
60	10273.0
61	10273.0
62	9311.0
63	8349.0
64	6591.5
65	4834.0
66	4169.0
67	3504.0
68	3504.0
69	3011.0
70	2518.0
71	2390.5
72	2263.0
73	2005.5
74	1748.0
75	1748.0
76	1079.0
77	410.0
78	308.5
79	207.0
80	158.5
81	110.0
82	110.0
83	90.5
84	71.0
85	63.5
86	56.0
87	46.5
88	37.0
89	37.0
90	26.5
91	16.0
92	9.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	260570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.32246336639654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.34590098798819	56.57602542249576
2	5.792576187780242	7.3360038689555065
3	1.699902830945927	3.2292610421702173
4	0.7569073815910822	1.9171695977022574
5	0.43091332127455806	1.3643246500250232
6	0.30011151569645383	1.1402280275113463
7	0.22807802675651004	1.0109723745778383
8	0.17201088138565962	0.871372218813201
9	0.14103014683716694	0.8037338675988626
>10	0.9377632784754237	11.719400500285152
>50	0.12341711185971035	5.552917647934338
>100	0.0689683860392499	7.300583071175211
>500	0.0018149575273486817	0.7742288076216142
>1k	6.049858424495606E-4	0.403778903133687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1054	0.4044978316767088	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	795	0.3051003569098515	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	632	0.24254518939248573	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	594	0.22796177610622864	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	442	0.16962812296120044	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	414	0.1588824500134321	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	409	0.15696357984418774	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	399	0.15312583950569902	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	387	0.1485205510995126	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	385	0.14775300303181488	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	365	0.14007752235483747	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	347	0.13316958974555781	No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	347	0.13316958974555781	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	345	0.13240204167786007	No Hit
CAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAG	300	0.11513221015466094	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	278	0.1066891814099858	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	276	0.10592163334228806	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	274	0.10515408527459033	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	272	0.10438653720689257	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	263	0.10093257090225274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.675480676977396E-4	0.0	0.0
2	0.0	0.0	7.675480676977396E-4	3.837740338488698E-4	0.0
3	0.0	0.0	0.0015350961353954792	3.837740338488698E-4	0.0
4	0.0	0.0	0.0015350961353954792	3.837740338488698E-4	0.0
5	0.0	0.0	0.0015350961353954792	3.837740338488698E-4	0.0
6	0.0	0.0	0.0026864182369420887	3.837740338488698E-4	0.0
7	0.0	0.0	0.0072917066431285255	3.837740338488698E-4	0.0
8	0.0	0.0	0.010361898913919485	3.837740338488698E-4	0.0
9	0.0	0.0	0.011129446981617224	7.675480676977396E-4	0.0
10	3.837740338488698E-4	0.0	0.013815865218559313	7.675480676977396E-4	0.0
11	3.837740338488698E-4	0.0	0.01995624976014123	7.675480676977396E-4	0.0
12	3.837740338488698E-4	0.0	0.021875119929385577	7.675480676977396E-4	0.0
13	3.837740338488698E-4	0.0	0.02302644203093219	7.675480676977396E-4	0.0
14	3.837740338488698E-4	0.0	0.024945312200176536	7.675480676977396E-4	0.0
15	3.837740338488698E-4	0.0	0.025712860267874275	7.675480676977396E-4	0.0
16	3.837740338488698E-4	0.0	0.028399278504816366	7.675480676977396E-4	0.0
17	3.837740338488698E-4	0.0	0.029166826572514102	7.675480676977396E-4	0.0
18	3.837740338488698E-4	0.0	0.029550600606362974	7.675480676977396E-4	0.0
19	3.837740338488698E-4	0.0	0.029550600606362974	7.675480676977396E-4	0.0
20	3.837740338488698E-4	0.0	0.02993437464021184	7.675480676977396E-4	0.0
21	3.837740338488698E-4	0.0	0.03146947077560732	0.0011513221015466094	0.0
22	3.837740338488698E-4	0.0	0.03146947077560732	0.0011513221015466094	0.0
23	3.837740338488698E-4	0.0	0.03146947077560732	0.0015350961353954792	0.0
24	3.837740338488698E-4	0.0	0.03185324480945619	0.0026864182369420887	0.0
25	3.837740338488698E-4	0.0	0.03185324480945619	0.0034539663046398283	0.0
26	3.837740338488698E-4	0.0	0.03185324480945619	0.0034539663046398283	0.0
27	3.837740338488698E-4	0.0	0.03185324480945619	0.005372836473884177	0.0
28	3.837740338488698E-4	0.0	0.03185324480945619	0.01535096135395479	0.0
29	3.837740338488698E-4	0.0	0.03185324480945619	0.04106382162182907	0.0
30	3.837740338488698E-4	0.0	0.03185324480945619	0.06562535978815673	0.0
31	3.837740338488698E-4	0.0	0.03185324480945619	0.13316958974555781	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCTA	25	0.0054886416	29.6	6
GGTATCA	240	0.0	24.666668	1
GTATCAA	575	0.0	23.808695	1
GATAGCA	40	0.0019272029	23.125002	2
ATAGATC	50	2.694338E-4	22.2	6
ATTATAG	70	5.0769795E-6	21.142857	3
TAAGGAT	45	0.0038179914	20.555555	24
AGGGCCA	110	1.7371349E-9	20.181818	5
GTAATAC	50	0.0070206453	18.5	3
CGCCTCT	50	0.0070206453	18.5	34
TATAGAT	60	9.2107063E-4	18.5	5
ATGCCCG	50	0.0070206453	18.5	29
TATAAGC	50	0.0070206453	18.5	2
GTAGTGG	60	9.2107063E-4	18.5	24
CAGGGCC	135	1.1386874E-9	17.814814	4
CGCGCAC	115	6.348637E-8	17.69565	34
GCGAACT	75	2.0595457E-4	17.266666	23
GGTAGTG	75	2.0595457E-4	17.266666	23
CGAACTA	65	0.001575505	17.076923	24
GTTAGGT	65	0.001575505	17.076923	1
>>END_MODULE