FastQCFastQC Report
Fri 10 Feb 2017
ERR1633105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429344
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44861.0448498173958412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.7926045315644331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16670.3882667511366177No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13390.31187113363643143No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11850.27600245956622194No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG10870.2531769397033614No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10710.24945032421554744No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10700.24921741074755907No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT10670.24851867034359396No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10220.23803756428411715No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10010.23314638145636135No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9950.23174890064843107No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9750.22709063128866364No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9300.21660952522918686No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9280.21614369829321012No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8970.20892338078557054No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8900.20729298650965194No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8730.2033334575538496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.2024018036818961No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8490.19774353432212866No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8310.19355109189833794No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8170.1902903033465007No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT8010.18656368785868674No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7850.18283707237087277No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7690.1791104568830588No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7530.17538384139524485No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7410.17258887977938436No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7290.16979391816352388No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7280.1695610046955355No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7240.16862935082358202No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7190.16746478348364016No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6910.16094320637996573No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6770.1576824178281285No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6750.15721659089215173No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6730.156750763956175No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6670.15535328314824476No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA6520.15185958112841916No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6230.14510509055675636No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6170.14370760974882613No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6140.14300886934486098No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT6130.1427759558768726No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6080.14161138853693078No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6010.13998099426101215No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5980.13928225385704704No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5900.13741894611314004No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG5650.13159610941343072No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5610.13066445554147724No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA5330.12414287843780279No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT5290.12321122456584929No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG5230.12181374375791905No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC5210.12134791682194232No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC5110.11901878214205858No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5110.11901878214205858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.11808712827010509No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG5010.11668964746217485No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4940.11505925318625625No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4910.11436051278229113No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4870.11342885891033763No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4840.1127301185063725No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA4820.11226429157039576No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG4770.11109972423045389No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT4760.11086681076246552No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4750.11063389729447715No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT4650.10830476261459343No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4530.10550980099873294No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4530.10550980099873294No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4520.10527688753074459No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4500.10481106059476784No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4500.10481106059476784No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4480.10434523365879109No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA4460.10387940672281434No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT4450.10364649325482597No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC4430.10318066631884923No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4400.1024819259148841No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4350.10131735857494223No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT4350.10131735857494223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTG507.3086994E-933.3000038
CTAGTGC303.595755E-430.8333349
AGATCTC250.00549255629.65
ATTACAT250.00549255629.69
CTAGTGG250.00549255629.69
CGTACGG250.00549255629.622
GCCACGC250.00549255629.636
GCGTACG250.00549255629.621
GGTATCA7250.027.8137931
CGGGTCC358.8603096E-426.4285717
TAGTGCA358.8603096E-426.4285710
GAATCAA451.3213267E-424.6666661
GTATCAA21150.024.0543751
CGGGTGG701.9170875E-723.78571317
GGCAGCT1400.023.78571318
CCGGCAG1400.023.78571316
TTTAGAC400.001929227423.12527
CACTAGT400.001929227423.1252
ACCCCAA400.001929227423.12512
GCCGGCA1400.022.46428515
Produced by FastQC (version 0.11.5)