FastQCFastQC Report
Fri 10 Feb 2017
ERR1633104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330135
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15090.4570857376527784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.35985278749602434No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7190.21778969209565782No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6380.19325427476638346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6290.19052811728535296No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5430.16447816802217274No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4730.1432747209474912No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC4510.13661078043830555No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4450.13479334211761854No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT4220.12782649522165176No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4140.12540324412740242No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4110.12449452496705893No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4080.12358580580671544No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4000.12116255471246612No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT3840.11631605252396748No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC3570.10813758008087601No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT3500.10601723537340786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1900.030.1842121
GTATCAA6800.026.1176471
CTTAGGG555.132428E-420.1818183
TCGCCAT851.2398141E-619.58823613
GTATTAG851.2398141E-619.5882361
AACACTA1153.0267984E-919.30434832
AACCGGG701.2158594E-418.533
GCGAACC701.2158594E-418.530
GAACCGG701.2158594E-418.532
TCGGCCC500.007024524318.49999816
GCTTCGC500.007024524318.49999822
GTCGCCA1054.7717003E-717.61904712
ACGTGGG1054.7717003E-717.61904736
TTCTAAG752.061648E-417.2666662
CGAACCG752.061648E-417.26666631
CATTCGG752.061648E-417.26666637
GGCGAAC752.061648E-417.26666629
CGTGGGA1107.76623E-716.81818237
ATCAACG11000.016.481823
CAACACT1352.2024324E-816.44444331
Produced by FastQC (version 0.11.5)