FastQCFastQC Report
Fri 10 Feb 2017
ERR1633103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279815
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22490.8037453317370405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18070.6457838214534604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9040.32307060021800116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.1704697746725515No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3020.10792845272769508No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG2890.10328252595464861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA200.001839557437.027
GCACCGA250.00548932729.613
CCGCTCT851.8189894E-1228.29411928
GCCGGCA950.027.26315915
CTACGGG358.852566E-426.4285711
GGTATCA3150.025.841271
CGCTCTC901.4188117E-1024.66666629
GTATCAA9650.024.538861
GCCGCTC1002.0008883E-1124.05000127
TGCTCGC1002.0008883E-1124.05000110
CCGGCAG1103.6379788E-1223.54545416
TTCGCCG952.7830538E-1023.36842224
GTCGCCC502.6950284E-422.237
CGCTTCC502.6950284E-422.226
CACTAAC603.7133315E-521.58333432
CGCCGCT1201.0913936E-1121.58333426
TAGGTAC705.079195E-621.1428573
AGCTTCG1059.731593E-1021.14285721
GCTTCGC1151.3278623E-1020.91304422
GTCCAGA806.921473E-720.81251
Produced by FastQC (version 0.11.5)