FastQCFastQC Report
Fri 10 Feb 2017
ERR1633102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448818
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15190.3384445365381959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.25310927814838086No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7190.1601985660111671No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7160.15953014362169077No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6520.14527046597952845No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6380.14215116149530546No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6320.14081431671635272No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6200.1381406271584473No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5750.12811429131630192No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5470.12187568234785592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5280.11764234054783898No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5220.11630549576888628No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5150.11474584352677478No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5060.11274057635834571No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5030.11207215396886934No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4670.10405108529515306No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4530.10093178081093004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA250.00549281929.59999833
CGCAATA701.9174877E-723.78571334
GTATCAA7900.023.6518971
CGAGTCG551.8993102E-523.54545421
TTACTCG400.001929363723.12519
ACTCGCA400.001929363723.12521
TTAACGC502.6985476E-422.1999993
GGTATCA3050.021.8360651
TTAGCCG705.0904964E-621.14285729
GCGAACC705.0904964E-621.14285730
TGGCGCA806.9406815E-720.812531
CGAACCG759.245148E-619.73333431
CGATAGA851.2418495E-619.58823614
GTTAGCC701.21709716E-418.528
TAGCCGG801.6130954E-518.530
CCGTCCG609.224859E-418.528
CTAAACA701.21709716E-418.54
ATATAAC701.21709716E-418.53
GTCCGGC701.21709716E-418.515
GGCGATA902.146273E-618.512
Produced by FastQC (version 0.11.5)