##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633102.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448818
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65320018359335	33.0	33.0	33.0	27.0	33.0
2	31.16919998752278	33.0	33.0	33.0	27.0	33.0
3	31.607533565944326	33.0	33.0	33.0	27.0	33.0
4	31.739023390327482	33.0	33.0	33.0	27.0	33.0
5	31.85884033171575	33.0	33.0	33.0	33.0	33.0
6	34.749196779095314	37.0	37.0	37.0	27.0	37.0
7	34.93939637002081	37.0	37.0	37.0	27.0	37.0
8	35.1096056753517	37.0	37.0	37.0	33.0	37.0
9	35.18994113426823	37.0	37.0	37.0	33.0	37.0
10	35.216183397279075	37.0	37.0	37.0	33.0	37.0
11	35.259303325624195	37.0	37.0	37.0	33.0	37.0
12	35.20012789148385	37.0	37.0	37.0	33.0	37.0
13	35.236937912472314	37.0	37.0	37.0	33.0	37.0
14	35.200379663917225	37.0	37.0	37.0	33.0	37.0
15	35.21295937328717	37.0	37.0	37.0	33.0	37.0
16	35.20786822275399	37.0	37.0	37.0	33.0	37.0
17	35.17953156958946	37.0	37.0	37.0	33.0	37.0
18	35.19457552950194	37.0	37.0	37.0	33.0	37.0
19	35.19956418860206	37.0	37.0	37.0	33.0	37.0
20	35.20703937899104	37.0	37.0	37.0	33.0	37.0
21	35.19975134687112	37.0	37.0	37.0	33.0	37.0
22	35.187766533427805	37.0	37.0	37.0	33.0	37.0
23	35.18459375515242	37.0	37.0	37.0	33.0	37.0
24	35.176599423374284	37.0	37.0	37.0	33.0	37.0
25	35.186253670752954	37.0	37.0	37.0	33.0	37.0
26	35.12910578452736	37.0	37.0	37.0	33.0	37.0
27	35.0651399899291	37.0	37.0	37.0	33.0	37.0
28	35.05883008257245	37.0	37.0	37.0	27.0	37.0
29	35.08006808996074	37.0	37.0	37.0	33.0	37.0
30	35.04965932738883	37.0	37.0	37.0	27.0	37.0
31	35.02664554451916	37.0	37.0	37.0	27.0	37.0
32	35.0575779046295	37.0	37.0	37.0	27.0	37.0
33	35.04076485345953	37.0	37.0	37.0	27.0	37.0
34	34.97903604579139	37.0	37.0	37.0	27.0	37.0
35	34.97906055461234	37.0	37.0	37.0	27.0	37.0
36	34.9528962742136	37.0	37.0	37.0	27.0	37.0
37	34.96035809615479	37.0	37.0	37.0	27.0	37.0
38	34.82429849070225	37.0	37.0	37.0	27.0	37.0
39	34.895743931838744	37.0	37.0	37.0	27.0	37.0
40	34.91425254780334	37.0	37.0	37.0	27.0	37.0
41	34.94350716771609	37.0	37.0	37.0	27.0	37.0
42	34.84798069596139	37.0	37.0	37.0	27.0	37.0
43	34.63995650798319	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	10.0
16	18.0
17	19.0
18	16.0
19	15.0
20	31.0
21	84.0
22	270.0
23	643.0
24	1298.0
25	2341.0
26	3943.0
27	5720.0
28	8253.0
29	10987.0
30	14444.0
31	17887.0
32	23199.0
33	30138.0
34	42802.0
35	76792.0
36	209908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35377369000352	20.845643445672856	13.757469620202398	25.043113244121223
2	15.821557958905391	22.976796830786643	38.11634114496299	23.08530406534497
3	17.225022169342587	27.63770615260529	30.01261090241479	25.12466077563734
4	12.455159998039294	18.20760308187283	39.682454803506104	29.654782116581778
5	13.70577828874956	37.12640758614851	35.91388937163839	13.253924753463542
6	29.018221194337123	40.55586005908854	17.37809089653267	13.047827850041665
7	26.41939494405305	32.2344469250342	24.094844680917433	17.25131344999532
8	24.02176383300135	35.99877901510189	20.797071418704242	19.18238573319252
9	25.8298463965349	15.502720479125168	20.421640843281686	38.24579228105824
10	15.123724984292075	27.78765557531115	34.389440708705976	22.699178731690797
11	33.51447580088143	23.943558413432616	24.299827547023515	18.24213823866244
12	22.929338841133823	26.261201645210306	29.777771836245424	21.031687677410442
13	27.131264788845368	22.605153982237788	26.28259116167355	23.980990067243294
14	22.02674580787758	20.812445133662198	27.589134125636672	29.571674932823548
15	23.846637166958544	28.688243341398962	24.861525161646814	22.603594329995676
16	23.1467989251768	28.388790110913554	25.27550142819584	23.188909535713808
17	22.264704178531165	27.537665601646992	26.353443934958044	23.844186284863795
18	22.7132156018698	27.68315887508968	27.642830724257937	21.96079479878258
19	24.466264722003128	26.38753347682134	27.252249241340586	21.893952559834943
20	23.822573960937397	26.489356487484905	27.674246576563327	22.01382297501437
21	22.534524016416455	27.50223921500475	27.559277925573394	22.403958843005405
22	22.526725755205895	27.232642184582616	27.311738834003986	22.928893226207506
23	22.55546791795338	27.114108614182143	27.975259459290847	22.35516400857363
24	22.895249299270528	27.868757491900947	25.863713130935036	23.37228007789349
25	23.203837635745447	26.98733116764479	27.655753557121148	22.153077639488615
26	22.933794990397	27.443640852193983	27.414675881983342	22.207888275425674
27	22.861605372333553	27.26338961449853	26.610563747443283	23.264441265724635
28	22.415099216163345	26.983320633307933	28.085549153554446	22.516030996974273
29	24.01998137329608	26.889073076391767	26.780120226907123	22.310825323405034
30	23.341532647977576	26.629279574348626	27.393286365520098	22.635901412153704
31	22.99194773828144	26.742911380559605	27.52563399863642	22.739506882522537
32	22.12032494240427	26.720630634243726	27.6241148973526	23.534929525999402
33	22.212567232152008	26.73956926861222	28.11429131630193	22.93357218293384
34	23.200495523798065	26.245827930252354	27.88591366656418	22.667762879385407
35	22.715666483964547	27.191868418824555	27.253363278656384	22.839101818554514
36	23.547629551399453	27.01117156620278	26.75650263581229	22.684696246585474
37	22.522492413405878	26.474874002379583	27.457454914909828	23.545178669304708
38	22.854029918586154	27.061526052876665	27.002704882602746	23.08173914593443
39	22.372765798163176	26.956583737728877	27.632804388415792	23.03784607569215
40	22.379895636984255	27.369000352035794	27.94696291146968	22.304141099510268
41	22.662638307732756	26.35054743793698	27.581335864426116	23.405478389904147
42	22.084898555762024	27.702097509458177	27.33847572958304	22.87452820519676
43	23.310785218061664	25.94303258781956	27.589579740562993	23.156602453555784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	11.5
2	19.0
3	88.0
4	157.0
5	157.0
6	204.0
7	251.0
8	248.0
9	245.0
10	316.0
11	387.0
12	387.0
13	620.5
14	854.0
15	1478.5
16	2103.0
17	2342.5
18	2582.0
19	2582.0
20	2340.5
21	2099.0
22	2310.5
23	2522.0
24	3495.0
25	4468.0
26	4468.0
27	5870.0
28	7272.0
29	9366.5
30	11461.0
31	14211.5
32	16962.0
33	16962.0
34	20631.5
35	24301.0
36	27216.5
37	30132.0
38	33121.5
39	36111.0
40	36111.0
41	37924.0
42	39737.0
43	40526.0
44	41315.0
45	39847.5
46	38380.0
47	38380.0
48	36433.0
49	34486.0
50	33712.0
51	32938.0
52	30349.5
53	27761.0
54	27761.0
55	27151.5
56	26542.0
57	22238.5
58	17935.0
59	16771.0
60	15607.0
61	15607.0
62	13535.5
63	11464.0
64	9414.5
65	7365.0
66	6186.5
67	5008.0
68	5008.0
69	4157.0
70	3306.0
71	2647.0
72	1988.0
73	1688.5
74	1389.0
75	1389.0
76	1054.0
77	719.0
78	583.5
79	448.0
80	328.0
81	208.0
82	208.0
83	170.0
84	132.0
85	115.0
86	98.0
87	69.0
88	40.0
89	40.0
90	28.5
91	17.0
92	11.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	448818.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.610502977143355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.88254367922738	51.50840087950287
2	6.535726654443175	7.661244530760736
3	1.8095442792097356	3.181749010916848
4	0.9025424758538519	2.115938738721221
5	0.5455517574315968	1.5987531451565193
6	0.38323273949407366	1.3476878171433722
7	0.2535392033381382	1.040204216245075
8	0.18667969167867132	0.8753112499923974
9	0.14824665132955042	0.7819929719191934
>10	1.1089290146638433	12.577926970453202
>50	0.15580155394230086	6.482768191687957
>100	0.08192022737543571	8.510792752997897
>500	0.004976462410657309	1.7215435845768294
>1k	7.656096016395859E-4	0.5956859399259067
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1519	0.3384445365381959	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.25310927814838086	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	719	0.1601985660111671	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	716	0.15953014362169077	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	652	0.14527046597952845	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	638	0.14215116149530546	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	632	0.14081431671635272	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	620	0.1381406271584473	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	575	0.12811429131630192	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	547	0.12187568234785592	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	528	0.11764234054783898	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	522	0.11630549576888628	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	515	0.11474584352677478	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	506	0.11274057635834571	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	503	0.11207215396886934	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	467	0.10405108529515306	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	453	0.10093178081093004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.684223894763579E-4	0.0	0.0
2	0.0	0.0	8.912298526351439E-4	0.0	0.0
3	0.0	0.0	8.912298526351439E-4	0.0	0.0
4	0.0	0.0	8.912298526351439E-4	2.2280746315878598E-4	0.0
5	0.0	0.0	8.912298526351439E-4	4.4561492631757196E-4	0.0
6	0.0	0.0	0.0015596522421115018	4.4561492631757196E-4	0.0
7	0.0	0.0	0.004010534336858148	4.4561492631757196E-4	0.0
8	0.0	0.0	0.005792994042128435	4.4561492631757196E-4	0.0
9	0.0	0.0	0.007352646284239937	8.912298526351439E-4	0.0
10	0.0	0.0	0.008466683600033867	0.0013368447789527157	0.0
11	0.0	0.0	0.010694758231621726	0.0015596522421115018	0.0
12	0.0	0.0	0.011808795547415657	0.0017824597052702878	0.0
13	0.0	0.0	0.012922832863209586	0.0017824597052702878	0.0
14	0.0	0.0	0.014036870179003515	0.0017824597052702878	0.0
15	0.0	0.0	0.01492810003163866	0.002005267168429074	0.0
16	0.0	0.0	0.016710559736908947	0.002005267168429074	0.0
17	0.0	0.0	0.01715617466322652	0.002005267168429074	0.0
18	0.0	0.0	0.01715617466322652	0.0022280746315878597	0.0
19	0.0	0.0	0.01715617466322652	0.0022280746315878597	0.0
20	0.0	0.0	0.01715617466322652	0.0022280746315878597	0.0
21	0.0	0.0	0.018047404515861662	0.0022280746315878597	0.0
22	0.0	0.0	0.01871582690533802	0.0024508820947466456	0.0
23	0.0	0.0	0.019161441831655593	0.0028964970210642177	0.0
24	0.0	0.0	0.019161441831655593	0.0033421119473817894	0.0
25	0.0	0.0	0.019161441831655593	0.0035649194105405757	0.0
26	0.0	0.0	0.019161441831655593	0.0037877268736993615	0.0
27	0.0	0.0	0.019161441831655593	0.004233341800016934	0.0
28	0.0	0.0	0.019161441831655593	0.01492810003163866	0.0
29	0.0	0.0	0.019161441831655593	0.02985620006327732	0.0
30	0.0	0.0	0.019161441831655593	0.053919406084426205	0.0
31	0.0	0.0	0.019161441831655593	0.11652830323204506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCTA	25	0.005492819	29.599998	33
CGCAATA	70	1.9174877E-7	23.785713	34
GTATCAA	790	0.0	23.651897	1
CGAGTCG	55	1.8993102E-5	23.545454	21
TTACTCG	40	0.0019293637	23.125	19
ACTCGCA	40	0.0019293637	23.125	21
TTAACGC	50	2.6985476E-4	22.199999	3
GGTATCA	305	0.0	21.836065	1
TTAGCCG	70	5.0904964E-6	21.142857	29
GCGAACC	70	5.0904964E-6	21.142857	30
TGGCGCA	80	6.9406815E-7	20.8125	31
CGAACCG	75	9.245148E-6	19.733334	31
CGATAGA	85	1.2418495E-6	19.588236	14
GTTAGCC	70	1.21709716E-4	18.5	28
TAGCCGG	80	1.6130954E-5	18.5	30
CCGTCCG	60	9.224859E-4	18.5	28
CTAAACA	70	1.21709716E-4	18.5	4
ATATAAC	70	1.21709716E-4	18.5	3
GTCCGGC	70	1.21709716E-4	18.5	15
GGCGATA	90	2.146273E-6	18.5	12
>>END_MODULE