FastQCFastQC Report
Fri 10 Feb 2017
ERR1633101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299529
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32751.0933832784137762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27180.9074246567110363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12770.42633601420897477No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7630.2547332645586905No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7170.23937582003745883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.23036166781847503No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6810.22735695041214707No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6710.22401837551622714No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6530.2180089407035713No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6500.2170073682347953No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5860.19564048890090774No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5760.19230191400498783No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5730.19130034153621187No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5720.19096648404661987No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5700.19029876906743587No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5620.18762790915069993No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5600.18696019417151594No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5480.18295390429641203No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5380.1796153294004921No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4950.16525945734803643No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4880.16292245492089247No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT4750.15858230755619657No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4700.1569130201082366No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4630.15457601768109266No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4530.15123744278517273No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4530.15123744278517273No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4380.14622958044129283No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4330.14456029299333287No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4320.14422643550374087No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4290.1432248630349649No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4200.14022014562863697No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4200.14022014562863697No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT4010.1338768533263891No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG3940.13153985089924514No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.1288689909825092No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3690.12319341365944533No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC3650.12185798370107737No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA3520.11751783633638145No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC3520.11751783633638145No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3450.11518083390923751No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA3340.11150840152372558No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG3220.10750211164862167No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG3210.10716825415902967No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC3150.10516510922147773No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC3100.10349582177351777No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA3100.10349582177351777No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3100.10349582177351777No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG3020.10082496185678182No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT3000.10015724687759783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGA556.2357867E-726.909094
GGTATCA5300.026.877361
GTATTAT509.061816E-625.8999981
CGCTCTC1350.024.66666829
ATTATAG601.3318968E-624.6666683
ATAATAG451.3200233E-424.6666663
ACCTAAG451.3200233E-424.6666661
CCGCTCT1400.023.78571328
GTATCAA15050.023.724251
GTCCGGC1250.023.6815
GCGGGCT551.896575E-523.54545431
AGAGGCC952.7830538E-1023.36842223
CTTAAGG400.001927872823.12519
ACTAGTC400.001927872823.1258
TATTATA802.708839E-823.1252
TAAGAAC400.001927872823.1254
CGAGTCG1054.0017767E-1122.9047621
GAGTCGC1054.0017767E-1122.9047622
TATACTT652.6718626E-622.769235
TCCGCCG903.799869E-922.6111131
Produced by FastQC (version 0.11.5)