##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633101.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	299529
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11860621175245	33.0	33.0	33.0	27.0	33.0
2	30.75490520116583	33.0	33.0	33.0	27.0	33.0
3	31.19742996504512	33.0	33.0	33.0	27.0	33.0
4	31.370164491585122	33.0	33.0	33.0	27.0	33.0
5	31.49617900103162	33.0	33.0	33.0	27.0	33.0
6	34.163222926661525	37.0	33.0	37.0	27.0	37.0
7	34.40158715850552	37.0	37.0	37.0	27.0	37.0
8	34.574555385288235	37.0	37.0	37.0	27.0	37.0
9	34.696132928698056	37.0	37.0	37.0	27.0	37.0
10	34.69270087370505	37.0	37.0	37.0	27.0	37.0
11	34.7159039692317	37.0	37.0	37.0	27.0	37.0
12	34.67295320319568	37.0	37.0	37.0	27.0	37.0
13	34.69620303877087	37.0	37.0	37.0	27.0	37.0
14	34.66313111585189	37.0	37.0	37.0	27.0	37.0
15	34.69800253063977	37.0	37.0	37.0	27.0	37.0
16	34.70361133646492	37.0	37.0	37.0	27.0	37.0
17	34.6847216797038	37.0	37.0	37.0	27.0	37.0
18	34.70005575420076	37.0	37.0	37.0	27.0	37.0
19	34.69881714291438	37.0	37.0	37.0	27.0	37.0
20	34.67806789993623	37.0	37.0	37.0	27.0	37.0
21	34.67364094962424	37.0	37.0	37.0	27.0	37.0
22	34.66312777727699	37.0	37.0	37.0	27.0	37.0
23	34.6411098758384	37.0	37.0	37.0	27.0	37.0
24	34.62626657185114	37.0	37.0	37.0	27.0	37.0
25	34.60077321394589	37.0	37.0	37.0	27.0	37.0
26	34.575309903214716	37.0	37.0	37.0	27.0	37.0
27	34.495718277695985	37.0	37.0	37.0	27.0	37.0
28	34.4433326990041	37.0	37.0	37.0	27.0	37.0
29	34.48468762623986	37.0	37.0	37.0	27.0	37.0
30	34.42399901178183	37.0	37.0	37.0	27.0	37.0
31	34.390873671664515	37.0	37.0	37.0	27.0	37.0
32	34.417361924888745	37.0	37.0	37.0	27.0	37.0
33	34.40627785623429	37.0	37.0	37.0	27.0	37.0
34	34.32170173839595	37.0	37.0	37.0	27.0	37.0
35	34.291090345175256	37.0	37.0	37.0	27.0	37.0
36	34.2588964674539	37.0	37.0	37.0	27.0	37.0
37	34.25364155056772	37.0	37.0	37.0	27.0	37.0
38	34.076727128258035	37.0	37.0	37.0	27.0	37.0
39	34.141605654210444	37.0	37.0	37.0	27.0	37.0
40	34.12751019099987	37.0	37.0	37.0	27.0	37.0
41	34.102464202130676	37.0	37.0	37.0	27.0	37.0
42	33.951176680722064	37.0	37.0	37.0	27.0	37.0
43	33.68981968356987	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	17.0
17	23.0
18	17.0
19	24.0
20	66.0
21	138.0
22	378.0
23	880.0
24	1684.0
25	2648.0
26	4129.0
27	5834.0
28	7928.0
29	9991.0
30	12587.0
31	15618.0
32	18937.0
33	23598.0
34	32340.0
35	54042.0
36	108644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46233252873679	20.24845674375436	13.640749309749639	23.648461417759215
2	16.74195153056966	24.137896497501078	37.344631070781126	21.775520901148134
3	18.307409299266514	28.033345686060446	30.297901037962937	23.361343976710103
4	12.326352373225964	18.478010476448024	39.1594803842032	30.03615676612281
5	14.46170487665635	36.69027039118082	34.18400221681373	14.664022515349098
6	28.043027553258614	41.459758487492024	16.241833011160857	14.2553809480885
7	26.267239566118803	32.9971388413142	22.105038243375432	18.630583349191564
8	24.73783840629789	34.1436054605731	20.395020181685247	20.72353595144377
9	26.96967572422036	13.869775547609747	19.870196208046632	39.290352520123264
10	16.434802640145026	27.009404765481804	32.1504762477089	24.405316346664264
11	34.314874352733796	23.75830053183498	22.839190862988225	19.087634252443003
12	24.570241946522707	26.310307182276173	28.24834990935769	20.871100961843428
13	28.18758784625195	22.730687178870827	25.813193380273695	23.268531594603527
14	22.86656717713477	20.784631872039103	28.577199536605804	27.771601414220328
15	25.231279775914857	27.102884862567567	26.73697705397474	20.928858307542843
16	22.145768857105654	28.03201025610208	25.658617362592608	24.163603524199658
17	22.282984285327963	26.166414604262023	25.795498933325323	25.755102177084687
18	23.01379833004484	25.44394699678495	29.20952562189304	22.332729051277173
19	25.93538522146436	24.54386720484494	28.126491925656616	21.39425564803408
20	24.90209629117715	24.321851974266266	29.161784000881386	21.614267733675202
21	23.144670465964897	26.625134794961426	28.49774145408291	21.73245328499077
22	22.73669661368348	26.51562953837525	27.95822775090225	22.78944609703902
23	22.656236958691814	26.297286740182084	29.532699671818087	21.513776629308012
24	23.532279011381203	26.587408898637527	26.776372237746592	23.103939852234674
25	23.173048352580217	26.38976526479907	29.062962183962153	21.374224198658563
26	23.55030731581917	26.536662560219543	28.426629808799813	21.486400315161468
27	24.020044803675102	25.7798076313145	27.800647015814828	22.39950054919557
28	22.031923453154786	26.111328118479342	29.174470585485878	22.682277842879987
29	24.348226715944033	26.054572345248705	27.53790117150593	22.05929976730133
30	23.494219257567714	25.353471617105523	29.37077878936597	21.78153033596079
31	22.772085507580233	26.096304531447707	28.23699875471156	22.894611206260496
32	20.9138347205112	25.728727435406924	29.199176039715685	24.158261804366187
33	21.53347422119394	25.829886254753298	29.884919323337638	22.751720200715123
34	22.054959619936636	26.135699715219562	29.58144286529852	22.227897799545286
35	22.37446123747617	26.867515332405212	28.050706275519232	22.707317154599387
36	23.189407369570226	26.783049387538437	27.93151915173489	22.096024091156448
37	22.334732196214723	26.195794063346124	28.321464699578335	23.148009040860817
38	22.800797251685147	25.91201519719293	28.03568268848759	23.251504862634338
39	22.155450724303822	26.3967762720805	28.461017130227788	22.986755873387885
40	21.89838045731799	27.761919547022156	28.705400812609128	21.63429918305072
41	22.7109895869849	25.340117317521845	28.59756484347092	23.35132825202234
42	21.632963753092355	27.466455668733246	28.322800129536706	22.577780448637693
43	22.98909287581503	25.233616778342	28.256696346597494	23.52059399924548
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	15.0
2	28.0
3	172.5
4	317.0
5	317.0
6	431.0
7	545.0
8	554.0
9	563.0
10	717.0
11	871.0
12	871.0
13	1414.5
14	1958.0
15	3365.5
16	4773.0
17	4902.5
18	5032.0
19	5032.0
20	3997.5
21	2963.0
22	2508.0
23	2053.0
24	2327.0
25	2601.0
26	2601.0
27	3145.0
28	3689.0
29	4462.5
30	5236.0
31	6672.0
32	8108.0
33	8108.0
34	10559.0
35	13010.0
36	14323.0
37	15636.0
38	17875.0
39	20114.0
40	20114.0
41	21137.0
42	22160.0
43	23589.5
44	25019.0
45	24332.0
46	23645.0
47	23645.0
48	23794.0
49	23943.0
50	24426.0
51	24909.0
52	23603.0
53	22297.0
54	22297.0
55	21561.5
56	20826.0
57	17681.0
58	14536.0
59	13062.5
60	11589.0
61	11589.0
62	10082.5
63	8576.0
64	6480.5
65	4385.0
66	3730.5
67	3076.0
68	3076.0
69	2614.0
70	2152.0
71	1859.0
72	1566.0
73	1542.0
74	1518.0
75	1518.0
76	1269.0
77	1020.0
78	717.5
79	415.0
80	265.5
81	116.0
82	116.0
83	99.5
84	83.0
85	99.5
86	116.0
87	87.0
88	58.0
89	58.0
90	41.5
91	25.0
92	12.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	299529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.11602214176136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85739185530045	29.563633767128668
2	8.067249778411771	5.8271394324047785
3	2.6962823230389605	2.9213697623794452
4	1.4023363502683595	2.0258724270595554
5	0.8889779234059212	1.6053173182632643
6	0.696414790636977	1.5091039199097098
7	0.47819369887030966	1.2089317951515606
8	0.4154251538420898	1.2002803243524427
9	0.2928888506478457	0.9520182193565369
>10	2.536375762592848	18.836346672131242
>50	0.36660890130891055	9.370500411730832
>100	0.2829264347057897	18.79820822499235
>500	0.015939517448213505	3.5655682337679524
>1k	0.002988659521540032	2.6157094913716463
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3275	1.0933832784137762	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2718	0.9074246567110363	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1277	0.42633601420897477	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	763	0.2547332645586905	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	717	0.23937582003745883	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	690	0.23036166781847503	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	681	0.22735695041214707	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	671	0.22401837551622714	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	653	0.2180089407035713	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	650	0.2170073682347953	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	586	0.19564048890090774	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	576	0.19230191400498783	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	573	0.19130034153621187	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	572	0.19096648404661987	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	570	0.19029876906743587	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	562	0.18762790915069993	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	560	0.18696019417151594	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	548	0.18295390429641203	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	538	0.1796153294004921	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	495	0.16525945734803643	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	488	0.16292245492089247	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	475	0.15858230755619657	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	470	0.1569130201082366	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	463	0.15457601768109266	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	453	0.15123744278517273	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	453	0.15123744278517273	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	438	0.14622958044129283	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	433	0.14456029299333287	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	432	0.14422643550374087	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	429	0.1432248630349649	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	420	0.14022014562863697	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	420	0.14022014562863697	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	401	0.1338768533263891	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	394	0.13153985089924514	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	386	0.1288689909825092	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	369	0.12319341365944533	No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC	365	0.12185798370107737	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	352	0.11751783633638145	No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC	352	0.11751783633638145	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	345	0.11518083390923751	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA	334	0.11150840152372558	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG	322	0.10750211164862167	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	321	0.10716825415902967	No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC	315	0.10516510922147773	No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC	310	0.10349582177351777	No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA	310	0.10349582177351777	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	310	0.10349582177351777	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	302	0.10082496185678182	No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT	300	0.10015724687759783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.338574895919928E-4	0.0	0.0
2	0.0	0.0	6.677149791839855E-4	3.338574895919928E-4	0.0
3	0.0	0.0	6.677149791839855E-4	3.338574895919928E-4	0.0
4	0.0	0.0	0.0023370024271439495	3.338574895919928E-4	0.0
5	0.0	0.0	0.004674004854287899	3.338574895919928E-4	0.0
6	0.0	0.0	0.010349582177351775	3.338574895919928E-4	0.0
7	0.0	0.0	0.021033021844295546	3.338574895919928E-4	0.0
8	0.0	0.0	0.02403773925062348	3.338574895919928E-4	0.0
9	0.0	0.0	0.028377886615319384	6.677149791839855E-4	0.0
10	0.0040062898751039135	0.0	0.031048746532055325	6.677149791839855E-4	0.0
11	0.0040062898751039135	0.0	0.04840933599083895	6.677149791839855E-4	0.0
12	0.0040062898751039135	0.0	0.052415625865942866	6.677149791839855E-4	0.0
13	0.0040062898751039135	0.0	0.05708963072023076	6.677149791839855E-4	0.0
14	0.0040062898751039135	0.0	0.05942663314737471	0.0010015724687759784	0.0
15	0.004340147364695906	0.0	0.06543606796003058	0.0010015724687759784	0.0
16	0.004340147364695906	0.0	0.06977621532472648	0.0010015724687759784	0.0
17	0.004674004854287899	0.0	0.07177936026227845	0.0010015724687759784	0.0
18	0.004674004854287899	0.0	0.0737825051998304	0.0010015724687759784	0.0
19	0.004674004854287899	0.0	0.07511793515819837	0.001335429958367971	0.0
20	0.004674004854287899	0.0	0.07611950762697435	0.001335429958367971	0.0
21	0.004674004854287899	0.0	0.07778879507493432	0.0023370024271439495	0.0
22	0.004674004854287899	0.0	0.07879036754371029	0.003004717406327935	0.0
23	0.004674004854287899	0.0	0.07979194001248627	0.0033385748959199276	0.0
24	0.004674004854287899	0.0	0.07979194001248627	0.005341719833471884	0.0
25	0.004674004854287899	0.0	0.07979194001248627	0.005341719833471884	0.0
26	0.004674004854287899	0.0	0.07979194001248627	0.0063432923022478625	0.0
27	0.004674004854287899	0.0	0.07979194001248627	0.010349582177351775	0.0
28	0.004674004854287899	0.0	0.07979194001248627	0.026040884188175434	0.0
29	0.004674004854287899	0.0	0.07979194001248627	0.06844078536635852	0.0
30	0.004674004854287899	0.0	0.07979194001248627	0.12319341365944533	0.0
31	0.004674004854287899	0.0	0.07979194001248627	0.22802466539133107	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATAGA	55	6.2357867E-7	26.90909	4
GGTATCA	530	0.0	26.87736	1
GTATTAT	50	9.061816E-6	25.899998	1
CGCTCTC	135	0.0	24.666668	29
ATTATAG	60	1.3318968E-6	24.666668	3
ATAATAG	45	1.3200233E-4	24.666666	3
ACCTAAG	45	1.3200233E-4	24.666666	1
CCGCTCT	140	0.0	23.785713	28
GTATCAA	1505	0.0	23.72425	1
GTCCGGC	125	0.0	23.68	15
GCGGGCT	55	1.896575E-5	23.545454	31
AGAGGCC	95	2.7830538E-10	23.368422	23
CTTAAGG	40	0.0019278728	23.125	19
ACTAGTC	40	0.0019278728	23.125	8
TATTATA	80	2.708839E-8	23.125	2
TAAGAAC	40	0.0019278728	23.125	4
CGAGTCG	105	4.0017767E-11	22.90476	21
GAGTCGC	105	4.0017767E-11	22.90476	22
TATACTT	65	2.6718626E-6	22.76923	5
TCCGCCG	90	3.799869E-9	22.61111	31
>>END_MODULE