FastQCFastQC Report
Fri 10 Feb 2017
ERR1633100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335219
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12370.3690124963083835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.25774195376753706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4750.14169841208284734No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3470.10351441893210112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG250.0054908629.59999820
AGTACGC250.0054908629.59999821
GGTATCA1900.025.315791
GTATCAA6400.024.5703141
CGGTACA400.001928349423.12500233
CCATGCG450.00382024220.5555559
ACTGTGC851.2399305E-619.5882368
CAGCGCA801.6111095E-518.59
TAACCTG609.21822E-418.55
TCAACTC609.21822E-418.513
TCCTCGC500.007024744518.49999818
GTCTACG500.007024744518.4999981
GTATTAG1750.017.9714281
ATCAACG9500.017.331583
TCAACGC9500.017.331584
TATGGCA650.001576779317.0769234
GCCGTTA650.001576779317.07692315
CAACGCA9700.016.9742265
AACGCAG9850.016.7157366
CGCAGAG10000.016.4658
Produced by FastQC (version 0.11.5)