FastQCFastQC Report
Fri 10 Feb 2017
ERR1633099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149418
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5780.38683425022420326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.29715295345942255No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC2730.18270891057302333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2700.18070112034694613No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC2580.17266995944263744No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA2550.17066216921656024No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT2290.15326132059055803No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA2070.1385375255993254No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG1930.1291678378776319No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA1830.12247520379070796No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT1770.11845962333855359No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA1680.11243625266032205No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG1560.10440509175601333No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG1530.10239730152993615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT250.00548123929.627
GGTATCA702.0372681E-1029.0714281
GTCGCCA405.9061276E-527.75000212
AGAGGTC509.022158E-625.9000028
CCCAGAA400.001923375323.1250022
GTATCGC400.001923375323.1250026
CTACACA400.001923375323.1250024
TCTATTC400.001923375323.1250023
GTATCAA2150.022.3720931
TATGCTG502.6868848E-422.25
TGTTGAA502.6868848E-422.220
GGTACCG450.003810481220.5555556
TACTGTG450.003810481220.5555557
CCGTTAA450.003810481220.55555516
TCGCCAT555.1143666E-420.18181813
GTTGAAG555.1143666E-420.18181821
ACCGGGG555.1143666E-420.18181834
CGAACCG555.1143666E-420.18181831
GCGAACC555.1143666E-420.18181830
AGGTTAA555.1143666E-420.18181819
Produced by FastQC (version 0.11.5)