##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633099.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	149418
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.174443507475672	33.0	33.0	33.0	14.0	33.0
2	30.13961503968732	33.0	33.0	33.0	14.0	33.0
3	30.62319131563801	33.0	33.0	33.0	27.0	33.0
4	30.793257840420832	33.0	33.0	33.0	27.0	33.0
5	30.90195960326065	33.0	33.0	33.0	27.0	33.0
6	32.86361080994258	37.0	33.0	37.0	14.0	37.0
7	33.156426936513675	37.0	33.0	37.0	14.0	37.0
8	33.25738532171492	37.0	33.0	37.0	27.0	37.0
9	33.17845908792783	37.0	33.0	37.0	14.0	37.0
10	33.125306188009475	37.0	33.0	37.0	14.0	37.0
11	33.267377424406696	37.0	33.0	37.0	27.0	37.0
12	33.35789530043235	37.0	33.0	37.0	27.0	37.0
13	33.38490007897308	37.0	33.0	37.0	27.0	37.0
14	33.43323428234885	37.0	33.0	37.0	27.0	37.0
15	33.49669383876106	37.0	33.0	37.0	27.0	37.0
16	33.48593208314929	37.0	33.0	37.0	27.0	37.0
17	33.48310109893052	37.0	33.0	37.0	27.0	37.0
18	33.47872411623767	37.0	33.0	37.0	27.0	37.0
19	33.44730219919956	37.0	33.0	37.0	27.0	37.0
20	33.45548729068787	37.0	33.0	37.0	27.0	37.0
21	33.452683077005446	37.0	33.0	37.0	27.0	37.0
22	33.48296055361469	37.0	33.0	37.0	27.0	37.0
23	33.44412989064236	37.0	33.0	37.0	27.0	37.0
24	33.42927893560348	37.0	33.0	37.0	27.0	37.0
25	33.38065025632788	37.0	33.0	37.0	27.0	37.0
26	33.30930677696128	37.0	33.0	37.0	22.0	37.0
27	33.269017119758	37.0	33.0	37.0	22.0	37.0
28	33.204459971355526	37.0	33.0	37.0	14.0	37.0
29	33.22424339771647	37.0	33.0	37.0	14.0	37.0
30	33.23288358832269	37.0	33.0	37.0	22.0	37.0
31	33.218501117669895	37.0	33.0	37.0	14.0	37.0
32	33.200912875289454	37.0	33.0	37.0	14.0	37.0
33	33.2320068532573	37.0	33.0	37.0	14.0	37.0
34	33.082332784537336	37.0	33.0	37.0	14.0	37.0
35	33.077460546922055	37.0	33.0	37.0	14.0	37.0
36	33.05324659679557	37.0	33.0	37.0	14.0	37.0
37	33.116063660335435	37.0	33.0	37.0	14.0	37.0
38	32.97990871247105	37.0	33.0	37.0	14.0	37.0
39	33.060996667068224	37.0	33.0	37.0	14.0	37.0
40	33.06185332423135	37.0	33.0	37.0	14.0	37.0
41	33.08329652384585	37.0	33.0	37.0	14.0	37.0
42	32.98504196281573	37.0	33.0	37.0	14.0	37.0
43	32.64027761046192	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	13.0
16	13.0
17	20.0
18	20.0
19	34.0
20	117.0
21	266.0
22	570.0
23	1169.0
24	1825.0
25	2740.0
26	3649.0
27	4928.0
28	6157.0
29	7459.0
30	9013.0
31	10452.0
32	12504.0
33	14825.0
34	19313.0
35	26437.0
36	27892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.31843552985584	22.854676143436535	13.064021737675514	23.76286658903211
2	15.883628478496567	26.083202826968638	36.05121203603314	21.98195665850165
3	18.63095477117884	28.534714693008873	31.199052323013294	21.635278212798994
4	14.373100965077834	20.785313683759654	37.46938119905232	27.372204152110186
5	14.7719819566585	38.4036729175869	34.44765690880617	12.376688216948427
6	27.688096481012998	41.267451043381655	17.79236772008727	13.252084755518077
7	24.46492390475043	34.16188143329452	24.055334698630688	17.317859963324363
8	23.49649975237254	37.35426789275723	21.004832081810758	18.14440027305947
9	25.112101620955972	16.52478282402388	20.77929031308142	37.583825241938726
10	14.110080445461726	29.479714626082533	34.559423898057794	21.850781030397943
11	32.28660536213843	24.830341725896478	25.045175280086735	17.837877631878353
12	21.68948854890308	26.917105034199363	30.74529173191985	20.648114684977713
13	26.889665234442973	23.24217965706943	27.550228218822365	22.317926889665234
14	21.045657149740997	22.600356048133424	28.360706206748855	27.993280595376728
15	23.117027399643952	29.794268428167957	25.591963484988423	21.49674068719967
16	22.164665569074675	29.96225354374975	25.60802580679704	22.265055080378534
17	21.805940382015553	29.025284771580402	26.736403913852413	22.432370932551635
18	21.255805860070407	28.756910144694746	28.786357734677214	21.200926260557633
19	23.653107390006557	27.63321688150022	28.825844275790065	19.887831452703157
20	23.54937156165924	27.041588028216147	28.801081529668448	20.60795888045617
21	21.96790212691911	28.748209720381745	27.54621263837021	21.73767551432893
22	21.489378789704052	28.182682140036675	28.18134361321929	22.146595457039982
23	21.618546627581683	28.287756495201382	28.46644982532225	21.627247051894685
24	22.71078451056767	28.44034855238325	27.398974688457884	21.4498922485912
25	22.771018217349983	27.511410941118204	28.509282683478567	21.208288158053247
26	22.296510460587076	28.02741302922004	27.708843646682464	21.967232863510418
27	22.1010855452489	27.841357801603557	27.98324164424634	22.074315008901205
28	20.8957421461939	28.576878287756497	28.873361977807228	21.65401758824238
29	22.617756896759428	28.447710449878866	27.63857098876976	21.29596166459195
30	21.91971516149326	28.448379713287558	28.048829458298197	21.583075666920987
31	22.51201327818603	27.990603541741958	28.252285534540682	21.245097645531327
32	21.540242808764674	27.91229972292495	28.304488080418693	22.242969387891687
33	21.296630928000642	27.7757699875517	29.02193845453694	21.90566062991072
34	21.60449209599914	27.88485992316856	28.368068104244472	22.142579876587828
35	21.99199560963204	28.503928576209027	27.943755103133487	21.560320711025447
36	22.357413430778088	28.34598241175762	27.50672609725736	21.789878060206934
37	22.9630968156447	27.122568900667925	27.880175079307712	22.034159204379662
38	22.89817826500154	28.09300084327189	27.569636857674446	21.439184034052122
39	22.14057208636175	28.06355325328943	28.246931427271143	21.548943233077676
40	22.189428315196295	27.989934278333266	28.10906316508051	21.711574241389926
41	21.9163688444498	27.782462621638626	28.331258616766387	21.969909917145188
42	22.1010855452489	27.85340454296002	28.040129033985195	22.005380877805887
43	21.923061478536724	27.4853096681792	28.212129730019143	22.379499123264935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	4.0
2	6.0
3	21.0
4	36.0
5	36.0
6	66.5
7	97.0
8	89.5
9	82.0
10	136.0
11	190.0
12	190.0
13	266.5
14	343.0
15	671.5
16	1000.0
17	1117.0
18	1234.0
19	1234.0
20	1311.5
21	1389.0
22	1633.0
23	1877.0
24	2317.5
25	2758.0
26	2758.0
27	3367.5
28	3977.0
29	5075.5
30	6174.0
31	6635.0
32	7096.0
33	7096.0
34	7829.0
35	8562.0
36	9193.5
37	9825.0
38	10625.5
39	11426.0
40	11426.0
41	12164.5
42	12903.0
43	12056.0
44	11209.0
45	11262.5
46	11316.0
47	11316.0
48	11161.5
49	11007.0
50	11098.0
51	11189.0
52	11125.5
53	11062.0
54	11062.0
55	9257.5
56	7453.0
57	6462.5
58	5472.0
59	4710.5
60	3949.0
61	3949.0
62	3413.5
63	2878.0
64	2304.0
65	1730.0
66	1449.5
67	1169.0
68	1169.0
69	960.0
70	751.0
71	622.5
72	494.0
73	426.0
74	358.0
75	358.0
76	270.0
77	182.0
78	136.5
79	91.0
80	72.0
81	53.0
82	53.0
83	44.5
84	36.0
85	31.0
86	26.0
87	18.5
88	11.0
89	11.0
90	7.5
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	149418.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.7481294087727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.71063493925787	56.29174530511719
2	4.8593705003359755	6.098328180005087
3	1.5358853205627312	2.891217925551138
4	0.8287381208869737	2.080070674215958
5	0.5471591454504731	1.7166606432959883
6	0.38077156905617715	1.4335622214191062
7	0.27091310515481515	1.189950340655075
8	0.19731860021118425	0.9905098448647419
9	0.14505583583092463	0.8191784122394892
>10	1.35883187388675	16.965827410352166
>50	0.1247906822957219	5.205530792809434
>100	0.03946372004223685	3.9305839992504246
>500	0.0010665870281685635	0.38683425022420326
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	578	0.38683425022420326	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	444	0.29715295345942255	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	273	0.18270891057302333	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	270	0.18070112034694613	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	258	0.17266995944263744	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	255	0.17066216921656024	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	229	0.15326132059055803	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	207	0.1385375255993254	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	193	0.1291678378776319	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	183	0.12247520379070796	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	177	0.11845962333855359	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	168	0.11243625266032205	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	156	0.10440509175601333	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	153	0.10239730152993615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	6.692634086923931E-4	0.0	0.0
4	0.0	0.0	6.692634086923931E-4	6.692634086923931E-4	0.0
5	0.0	0.0	6.692634086923931E-4	6.692634086923931E-4	0.0
6	0.0	0.0	6.692634086923931E-4	6.692634086923931E-4	0.0
7	0.0	0.0	0.0026770536347695724	6.692634086923931E-4	0.0
8	0.0	0.0	0.0026770536347695724	6.692634086923931E-4	0.0
9	0.0	0.0	0.004015580452154359	0.0013385268173847862	0.0
10	0.0	0.0	0.004684843860846752	0.0013385268173847862	0.0
11	0.0	0.0	0.008031160904308717	0.0020077902260771793	0.0
12	0.0	0.0	0.008700424313001111	0.0020077902260771793	0.0
13	0.0	0.0	0.008700424313001111	0.0026770536347695724	0.0
14	0.0	0.0	0.008700424313001111	0.0026770536347695724	0.0
15	0.0	0.0	0.009369687721693503	0.0026770536347695724	0.0
16	0.0	0.0	0.009369687721693503	0.0026770536347695724	0.0
17	0.0	0.0	0.010038951130385897	0.0026770536347695724	0.0
18	0.0	0.0	0.010038951130385897	0.0026770536347695724	0.0
19	0.0	0.0	0.010038951130385897	0.0026770536347695724	0.0
20	0.0	0.0	0.01070821453907829	0.0026770536347695724	0.0
21	0.0	0.0	0.012046741356463078	0.0026770536347695724	0.0
22	0.0	0.0	0.012046741356463078	0.0026770536347695724	0.0
23	0.0	0.0	0.012046741356463078	0.003346317043461966	0.0
24	0.0	0.0	0.012046741356463078	0.003346317043461966	0.0
25	0.0	0.0	0.012046741356463078	0.003346317043461966	0.0
26	0.0	0.0	0.012046741356463078	0.003346317043461966	0.0
27	0.0	0.0	0.012046741356463078	0.004015580452154359	0.0
28	0.0	0.0	0.012046741356463078	0.008031160904308717	0.0
29	0.0	0.0	0.012046741356463078	0.01472379499123265	0.0
30	0.0	0.0	0.012046741356463078	0.0294475899824653	0.0
31	0.0	0.0	0.012046741356463078	0.06960339450400889	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	25	0.005481239	29.6	27
GGTATCA	70	2.0372681E-10	29.071428	1
GTCGCCA	40	5.9061276E-5	27.750002	12
AGAGGTC	50	9.022158E-6	25.900002	8
CCCAGAA	40	0.0019233753	23.125002	2
GTATCGC	40	0.0019233753	23.125002	6
CTACACA	40	0.0019233753	23.125002	4
TCTATTC	40	0.0019233753	23.125002	3
GTATCAA	215	0.0	22.372093	1
TATGCTG	50	2.6868848E-4	22.2	5
TGTTGAA	50	2.6868848E-4	22.2	20
GGTACCG	45	0.0038104812	20.555555	6
TACTGTG	45	0.0038104812	20.555555	7
CCGTTAA	45	0.0038104812	20.555555	16
TCGCCAT	55	5.1143666E-4	20.181818	13
GTTGAAG	55	5.1143666E-4	20.181818	21
ACCGGGG	55	5.1143666E-4	20.181818	34
CGAACCG	55	5.1143666E-4	20.181818	31
GCGAACC	55	5.1143666E-4	20.181818	30
AGGTTAA	55	5.1143666E-4	20.181818	19
>>END_MODULE