FastQCFastQC Report
Fri 10 Feb 2017
ERR1633098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414639
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35320.8518253227506336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.6762509074158485No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC19240.4640180976705037No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG17020.41047754793929175No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC17020.41047754793929175No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT15210.3668251177530334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14580.35163117796444615No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14400.34729005231056415No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC14320.345360663131061No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13650.32920202875272225No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG13620.3284785078104086No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT13260.3197962565026445No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC13220.31883156191289286No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG13050.3147316099064487No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT12200.29423184987422796No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG12120.2923024606947248No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA12060.29085541881009747No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC11430.2756614790215103No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA11200.2701144851304388No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC11170.2693909641881251No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT10960.26432631759192937No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC10600.25564406628416525No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10210.2462382940340875No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG9980.24069130014301598No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT9650.23273256977756554No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG9460.22815027047624561No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG9320.2247738394121151No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA9040.21802097728385417No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG8840.2131975043350963No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8480.2045152530273322No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC8460.20403290573245644No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA8110.1955918280721302No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG7980.19245657065543761No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT7880.19004483418105872No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7800.18811544500155558No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC7700.18570370852717666No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC7690.18546253487973877No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7660.1847390139374251No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG7640.1842566666425493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.17750380451428835No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG7310.1762979362770989No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC7290.17581558898222308No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT7220.17412737345015786No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA7030.1695450741488379No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7020.1693039005014No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA6980.16833920591164844No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT6960.16785685861677266No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC6910.1666509903795832No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG6900.1664098167321453No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG6620.15965695460388435No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6550.1579687390718191No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT6430.1550746553025644No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC6170.1488041404691792No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6090.14687475128967606No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG6050.14591005669992452No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT6040.14566888305248663No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA5970.14398066752042138No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA5780.13939836821910143No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT5710.13771015268703618No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC5460.1316808115010889No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG5400.13023376961646155No Hit
GGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCG5350.1290279013792721No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA5300.12782203314208263No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG5260.12685733855233106No Hit
GTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAG5090.12275738654588692No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT5050.12179269195613533No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT5050.12179269195613533No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT5040.12155151830869745No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT4980.1201044764240701No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA4980.1201044764240701No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4970.1198633027766322No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4880.11769273994969118No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCC4880.11769273994969118No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCT4830.11648687171250173No Hit
AAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGAC4820.11624569806506382No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCT4810.11600452441762593No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4720.11383396159068492No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCG4660.11238691970605755No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT4640.11190457241118178No Hit
AAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCT4630.11166339876374387No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG4570.11021635687911654No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4560.10997518323167864No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTG4550.10973400958424073No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCT4540.10949283593680287No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4540.10949283593680287No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4450.10732227310986182No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC4420.10659875216754815No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC4420.10659875216754815No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4390.10587523122523448No Hit
CTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGG4380.10563405757779659No Hit
CTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGC4310.10394584204573136No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA4280.10322232110341767No Hit
TTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGA4270.10298114745597978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG1250.028.11999921
ACACGAA358.859795E-426.4285717
TGGACGT358.859795E-426.4285726
GACGTAT358.859795E-426.4285728
GGACGTA358.859795E-426.4285727
ACGTATA358.859795E-426.4285729
GTTATAG358.859795E-426.4285711
GGTATCA6200.025.661291
GCCGTCC1100.025.22727227
AGTCGCC1200.024.66666823
CCTTAGG901.4188117E-1024.6666661
CGCTCTC751.3684257E-824.66666629
GGCGAGT1450.024.24137919
GTATCAA17400.023.8160931
GAGTCGC1400.023.78571322
TCCGCCG1103.6379788E-1223.54545431
GTCCGGC1650.023.54545415
GGTGTTT400.001929116323.12500216
GCCGCTC802.7152055E-823.12500227
ACTGTGC400.001929116323.1250028
Produced by FastQC (version 0.11.5)