FastQCFastQC Report
Fri 10 Feb 2017
ERR1633097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119153
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6380.5354460231802808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.4808943123547036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3390.2845081533826257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.12504930635401543No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1300.1091034216511544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG200.001835322937.034
CAATTAT352.3705168E-531.7142871
GCCGGCA405.8962338E-527.75000215
CCGCTCT405.8962338E-527.75000228
CGCTCTC405.8962338E-527.75000229
TTCGCCG405.8962338E-527.75000224
TGTAATA358.822625E-426.4285722
CCGGCAG451.3135133E-424.66666616
AGCTTCG451.3135133E-424.66666621
GCCGCTC451.3135133E-424.66666627
GCTTCGC451.3135133E-424.66666622
GGTATCA1800.024.6666661
CGCCGGC451.3135133E-424.66666614
CGCCGCT451.3135133E-424.66666626
TTATGCT400.001921100123.12500235
CTTCGCC502.6824564E-422.223
CAAACCA450.003806016620.5555559
TCGCCGC555.106004E-420.18181825
CGGCAGC555.106004E-420.18181817
GCTCTCT555.106004E-420.18181830
Produced by FastQC (version 0.11.5)