##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633097.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119153
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.651943299791025	33.0	27.0	33.0	14.0	33.0
2	29.774776967428433	33.0	33.0	33.0	14.0	33.0
3	30.2919355786258	33.0	33.0	33.0	14.0	33.0
4	30.420383876192794	33.0	33.0	33.0	14.0	33.0
5	30.611382004649485	33.0	33.0	33.0	27.0	33.0
6	31.7567581177142	37.0	27.0	37.0	14.0	37.0
7	32.207447567413325	37.0	33.0	37.0	14.0	37.0
8	32.356214279120124	37.0	33.0	37.0	14.0	37.0
9	32.109598583334034	37.0	27.0	37.0	14.0	37.0
10	31.987956660763892	37.0	27.0	37.0	14.0	37.0
11	32.17099863201094	37.0	27.0	37.0	14.0	37.0
12	32.35552608830663	37.0	33.0	37.0	14.0	37.0
13	32.47033645816723	37.0	33.0	37.0	14.0	37.0
14	32.52441818502262	37.0	33.0	37.0	14.0	37.0
15	32.62476815522899	37.0	33.0	37.0	14.0	37.0
16	32.61040846642552	37.0	33.0	37.0	14.0	37.0
17	32.61162538920548	37.0	33.0	37.0	14.0	37.0
18	32.60939296534708	37.0	33.0	37.0	14.0	37.0
19	32.610794524686746	37.0	33.0	37.0	14.0	37.0
20	32.61820516478813	37.0	33.0	37.0	14.0	37.0
21	32.63131436052806	37.0	33.0	37.0	14.0	37.0
22	32.63001351203914	37.0	33.0	37.0	14.0	37.0
23	32.550695324498754	37.0	33.0	37.0	14.0	37.0
24	32.55777026176428	37.0	33.0	37.0	14.0	37.0
25	32.538014149874535	37.0	33.0	37.0	14.0	37.0
26	32.435406578097066	37.0	33.0	37.0	14.0	37.0
27	32.39933530838502	37.0	33.0	37.0	14.0	37.0
28	32.304834960093324	37.0	33.0	37.0	14.0	37.0
29	32.373116916905154	37.0	33.0	37.0	14.0	37.0
30	32.342139937727126	37.0	33.0	37.0	14.0	37.0
31	32.30240111453342	37.0	33.0	37.0	14.0	37.0
32	32.31507389658674	37.0	33.0	37.0	14.0	37.0
33	32.28197359697196	37.0	33.0	37.0	14.0	37.0
34	32.10048425134071	37.0	27.0	37.0	14.0	37.0
35	32.164611885558905	37.0	27.0	37.0	14.0	37.0
36	32.083657146693746	37.0	27.0	37.0	14.0	37.0
37	32.12965682777605	37.0	27.0	37.0	14.0	37.0
38	32.00929057598214	37.0	27.0	37.0	14.0	37.0
39	32.10935519877804	37.0	27.0	37.0	14.0	37.0
40	32.07252020511443	37.0	27.0	37.0	14.0	37.0
41	32.08751772930602	37.0	27.0	37.0	14.0	37.0
42	31.92401366310542	37.0	27.0	37.0	14.0	37.0
43	31.56405629736557	37.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	5.0
16	15.0
17	25.0
18	25.0
19	77.0
20	178.0
21	407.0
22	831.0
23	1414.0
24	2185.0
25	3028.0
26	4138.0
27	5249.0
28	6355.0
29	7511.0
30	8823.0
31	10053.0
32	11553.0
33	13015.0
34	14879.0
35	16443.0
36	12942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91881866172065	23.754332664725187	12.08530209058941	23.24154658296476
2	16.301729708861714	27.180180104571434	34.3491141641419	22.16897602242495
3	19.2307369516504	29.631650063363914	29.873356105175695	21.26425687980999
4	14.58460970349047	22.462716003793442	37.142161758411454	25.810512534304635
5	16.095272464814148	37.56766510285095	33.865702080518325	12.471360351816573
6	27.788641494549026	41.79332454910913	17.38101432611852	13.037019630223327
7	25.238139199180885	33.99326915814122	23.876864199810328	16.891727442867573
8	24.260404689768617	36.760299782632416	20.656634746922027	18.322660780676944
9	25.71399796899784	15.511988787525283	21.22481179659765	37.54920144687922
10	14.956400594194019	29.228806660344265	33.98487658724497	21.829916158216747
11	33.106174414408365	24.59778603979757	24.560858727854104	17.73518081793996
12	22.19667150638255	26.962812518358746	31.05838711572516	19.78212885953354
13	27.857460575898212	23.252456925129874	26.681661393334622	22.208421105637292
14	21.58485308804646	22.242830646311884	28.881354225239818	27.290962040401833
15	23.33386486282343	29.87755239062382	25.44123941486996	21.347343331682794
16	21.547925776102993	29.57793760962796	26.345958557484916	22.528178056784135
17	21.617584114541806	28.532223275956127	27.326210838166055	22.523981771336015
18	21.989375005245357	27.63589670423741	29.11466769615536	21.26006059436187
19	23.73586900875345	27.39586917660487	28.978708047636232	19.889553767005445
20	23.512626622913395	27.095415138519385	28.72357389239046	20.66838434617676
21	21.989375005245357	27.940547027771018	29.22964591743389	20.84043204954974
22	22.11778133995787	27.756749725143305	29.051723414433546	21.073745520465284
23	21.92307369516504	27.617433048265678	29.260698429749986	21.198794826819302
24	21.71661645111747	28.371085914748267	28.510402591625894	21.40189504250837
25	22.14547682391547	27.9917417102381	28.982904333084353	20.87987713276208
26	21.9423766082264	28.393745856168117	28.562436531182595	21.101441004422885
27	21.95832249292926	28.11007695987512	28.476832308040922	21.4547682391547
28	21.72584827910334	27.672824016180876	29.21034300437253	21.390984700343257
29	22.212617391085413	27.858299832987836	28.526348476328756	21.402734299597995
30	22.288150529151597	27.41601134675585	28.78148263157453	21.51435549251802
31	22.184921907127812	27.91956560053041	28.735323491645197	21.160189000696583
32	21.351539617130914	27.995098738596596	28.79491074500852	21.85845089926397
33	21.07122774919641	28.010205366209835	29.23552071706126	21.6830461675325
34	21.829916158216747	27.559524309081603	28.952691077857878	21.657868454843772
35	21.659546969023022	28.26785729272448	28.75798343306505	21.314612305187445
36	22.199189277651424	28.164628670700697	28.178056784134686	21.458125267513196
37	22.209260362726916	27.73996458335082	28.699235436791355	21.351539617130914
38	22.17736859332119	27.976635082624863	28.286320948696215	21.559675375357735
39	21.961679521287756	27.794516294176397	28.676575495371498	21.567228689164352
40	21.698152795145738	28.21498409607815	29.09956106854213	20.987302040233985
41	21.815648787693135	27.478116371388044	28.650558525593144	22.055676315325673
42	21.512676978338774	28.21750186734702	28.678254009550745	21.591567144763456
43	21.898735239565937	27.484830428105038	28.578382415885457	22.038051916443564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	25.0
4	46.0
5	46.0
6	84.0
7	122.0
8	126.5
9	131.0
10	182.5
11	234.0
12	234.0
13	359.5
14	485.0
15	847.5
16	1210.0
17	1264.0
18	1318.0
19	1318.0
20	1309.0
21	1300.0
22	1386.5
23	1473.0
24	1854.0
25	2235.0
26	2235.0
27	2683.5
28	3132.0
29	3797.0
30	4462.0
31	5062.0
32	5662.0
33	5662.0
34	6423.5
35	7185.0
36	7740.5
37	8296.0
38	8541.5
39	8787.0
40	8787.0
41	9109.0
42	9431.0
43	9310.0
44	9189.0
45	9092.5
46	8996.0
47	8996.0
48	8715.5
49	8435.0
50	8354.5
51	8274.0
52	7692.0
53	7110.0
54	7110.0
55	6590.0
56	6070.0
57	5331.5
58	4593.0
59	4122.0
60	3651.0
61	3651.0
62	3169.0
63	2687.0
64	2177.0
65	1667.0
66	1432.0
67	1197.0
68	1197.0
69	967.5
70	738.0
71	618.5
72	499.0
73	382.0
74	265.0
75	265.0
76	188.0
77	111.0
78	89.0
79	67.0
80	51.0
81	35.0
82	35.0
83	28.0
84	21.0
85	22.0
86	23.0
87	13.5
88	4.0
89	4.0
90	4.0
91	4.0
92	2.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	119153.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.56206725806317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.21713108105145	70.60334192173089
2	4.0503803740161795	6.202109892323316
3	1.2518360993576392	2.8752947890527305
4	0.680726985727753	2.084714610626673
5	0.38695108850546994	1.481288763186827
6	0.24992874838313642	1.148103698605994
7	0.19621599105518164	1.0515891332992036
8	0.14688794861114157	0.8996836000772116
9	0.10852169337688816	0.7477780668552197
>10	0.6719575559599237	9.605297390749708
>50	0.03288536162936005	1.6768356650692806
>100	0.004384714883914673	0.6076221328879676
>500	0.0021923574419573364	1.0163403355349845
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	638	0.5354460231802808	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	573	0.4808943123547036	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	339	0.2845081533826257	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	149	0.12504930635401543	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	130	0.1091034216511544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	8.392570896242647E-4	0.0	0.0
6	0.0	0.0	0.0025177712688727936	0.0	0.0
7	0.0	0.0	0.010071085075491175	0.0	0.0
8	0.0	0.0	0.01091034216511544	0.0	0.0
9	0.0	0.0	0.015106627613236763	8.392570896242647E-4	0.0
10	0.0016785141792485293	0.0	0.019302913061358087	8.392570896242647E-4	0.0
11	0.0016785141792485293	0.0	0.024338455599103675	8.392570896242647E-4	0.0
12	0.0016785141792485293	0.0	0.02685622686797647	8.392570896242647E-4	0.0
13	0.0016785141792485293	0.0	0.02937399813684926	8.392570896242647E-4	0.0
14	0.0016785141792485293	0.0	0.030213255226473525	8.392570896242647E-4	0.0
15	0.0016785141792485293	0.0	0.031891769405722054	8.392570896242647E-4	0.0
16	0.0016785141792485293	0.0	0.033570283584970585	8.392570896242647E-4	0.0
17	0.0016785141792485293	0.0	0.033570283584970585	8.392570896242647E-4	0.0
18	0.0016785141792485293	0.0	0.033570283584970585	8.392570896242647E-4	0.0
19	0.0016785141792485293	0.0	0.03524879776421911	8.392570896242647E-4	0.0
20	0.0016785141792485293	0.0	0.03608805485384338	8.392570896242647E-4	0.0
21	0.0016785141792485293	0.0	0.037766569033091904	8.392570896242647E-4	0.0
22	0.0016785141792485293	0.0	0.037766569033091904	8.392570896242647E-4	0.0
23	0.0016785141792485293	0.0	0.037766569033091904	0.0016785141792485293	0.0
24	0.0016785141792485293	0.0	0.037766569033091904	0.0016785141792485293	0.0
25	0.0016785141792485293	0.0	0.037766569033091904	0.0016785141792485293	0.0
26	0.0016785141792485293	0.0	0.037766569033091904	0.0016785141792485293	0.0
27	0.0016785141792485293	0.0	0.037766569033091904	0.0025177712688727936	0.0
28	0.0016785141792485293	0.0	0.037766569033091904	0.00923182798586691	0.0
29	0.0016785141792485293	0.0	0.037766569033091904	0.028534741047224997	0.0
30	0.0016785141792485293	0.0	0.037766569033091904	0.0545517108255772	0.0
31	0.0016785141792485293	0.0	0.037766569033091904	0.10826416456153014	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGG	20	0.0018353229	37.0	34
CAATTAT	35	2.3705168E-5	31.714287	1
GCCGGCA	40	5.8962338E-5	27.750002	15
CCGCTCT	40	5.8962338E-5	27.750002	28
CGCTCTC	40	5.8962338E-5	27.750002	29
TTCGCCG	40	5.8962338E-5	27.750002	24
TGTAATA	35	8.822625E-4	26.428572	2
CCGGCAG	45	1.3135133E-4	24.666666	16
AGCTTCG	45	1.3135133E-4	24.666666	21
GCCGCTC	45	1.3135133E-4	24.666666	27
GCTTCGC	45	1.3135133E-4	24.666666	22
GGTATCA	180	0.0	24.666666	1
CGCCGGC	45	1.3135133E-4	24.666666	14
CGCCGCT	45	1.3135133E-4	24.666666	26
TTATGCT	40	0.0019211001	23.125002	35
CTTCGCC	50	2.6824564E-4	22.2	23
CAAACCA	45	0.0038060166	20.555555	9
TCGCCGC	55	5.106004E-4	20.181818	25
CGGCAGC	55	5.106004E-4	20.181818	17
GCTCTCT	55	5.106004E-4	20.181818	30
>>END_MODULE