FastQCFastQC Report
Fri 10 Feb 2017
ERR1633096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501054
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69341.383882775110068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51671.0312261752226306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26190.5226981522949622No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.27681647087938627No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10650.21255194050940618No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10560.21075572692763656No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10010.19977886615015547No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9110.18181673033245915No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8630.17223692456302114No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8520.17004155240752494No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8480.16924323526007176No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8250.16465291166221607No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8170.1630562773673097No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8130.16225796021985653No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8070.1610604844986768No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7930.15826637448259068No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7820.1560710023270945No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7820.1560710023270945No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7340.14649119655765647No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7300.14569287941020329No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7210.14389666582843366No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.1430983486809805No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6950.13870760436998805No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6570.13112359146918295No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6490.12952695717427665No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6370.1271320057319171No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6280.12533579215014748No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6230.12433789571583104No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6100.12174336498660823No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT6020.12014673069170188No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5940.11855009639679555No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5900.11775177924934238No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5890.1175521999624791No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG5770.11515724852011959No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5720.11415935208580312No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT5710.11395977279893983No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG5700.11376019351207653No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT5540.11056692492226387No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5520.11016776634853728No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC5340.10657533918499802No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA5220.10418038774263852No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC5110.1019850155871423No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.10118669843968914No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT5020.10018880200537267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG251.2326319E-437.016
GGTCGCT250.00549342129.628
GGTATCA11300.026.8495581
CGTTATT358.8623865E-426.4285710
GAGACGT358.8623865E-426.4285724
CCGCTCT2750.025.56363528
GCCGCTC2750.025.56363527
CCGGCAG2900.025.5172416
GCCGGCA2900.025.5172415
ACACGAT451.321757E-424.66666815
CACGATA451.321757E-424.66666816
CTAGTGC451.321757E-424.6666689
CGCTCTC2900.024.24137929
TGCTCGC3150.024.07936510
CGCCGTT701.9184154E-723.78571325
CGCCGGC3200.023.70312514
GCAGCTT3050.023.65573919
GTATCAA30250.023.5454561
GATTAAT551.899883E-523.5454541
TTCGCCG3000.023.43333424
Produced by FastQC (version 0.11.5)