##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501054
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79867838596239	33.0	33.0	33.0	33.0	33.0
2	31.268452102966947	33.0	33.0	33.0	27.0	33.0
3	31.7239798504752	33.0	33.0	33.0	27.0	33.0
4	31.837925652724056	33.0	33.0	33.0	27.0	33.0
5	31.9678936801223	33.0	33.0	33.0	33.0	33.0
6	34.81987769781301	37.0	37.0	37.0	27.0	37.0
7	35.01956475749121	37.0	37.0	37.0	33.0	37.0
8	35.18999948109386	37.0	37.0	37.0	33.0	37.0
9	35.26815273403665	37.0	37.0	37.0	33.0	37.0
10	35.28093179577451	37.0	37.0	37.0	33.0	37.0
11	35.33020592590819	37.0	37.0	37.0	33.0	37.0
12	35.248524111173644	37.0	37.0	37.0	33.0	37.0
13	35.29175298470823	37.0	37.0	37.0	33.0	37.0
14	35.24591560989434	37.0	37.0	37.0	33.0	37.0
15	35.28952767565971	37.0	37.0	37.0	33.0	37.0
16	35.292860649750324	37.0	37.0	37.0	33.0	37.0
17	35.27810176148679	37.0	37.0	37.0	33.0	37.0
18	35.27675460130046	37.0	37.0	37.0	33.0	37.0
19	35.278498924267645	37.0	37.0	37.0	33.0	37.0
20	35.27656300518507	37.0	37.0	37.0	33.0	37.0
21	35.27208644178073	37.0	37.0	37.0	33.0	37.0
22	35.28576560610234	37.0	37.0	37.0	33.0	37.0
23	35.282396707740084	37.0	37.0	37.0	33.0	37.0
24	35.27119033078271	37.0	37.0	37.0	33.0	37.0
25	35.27169127479274	37.0	37.0	37.0	33.0	37.0
26	35.23772687175434	37.0	37.0	37.0	33.0	37.0
27	35.16879218607176	37.0	37.0	37.0	33.0	37.0
28	35.14550926646629	37.0	37.0	37.0	33.0	37.0
29	35.18213605719144	37.0	37.0	37.0	33.0	37.0
30	35.11841039089599	37.0	37.0	37.0	33.0	37.0
31	35.09668418972805	37.0	37.0	37.0	33.0	37.0
32	35.10629792397626	37.0	37.0	37.0	33.0	37.0
33	35.08055419176376	37.0	37.0	37.0	33.0	37.0
34	34.995994443712654	37.0	37.0	37.0	27.0	37.0
35	34.976784937352065	37.0	37.0	37.0	27.0	37.0
36	34.924616907558864	37.0	37.0	37.0	27.0	37.0
37	34.928470783588196	37.0	37.0	37.0	27.0	37.0
38	34.753232186550754	37.0	37.0	37.0	27.0	37.0
39	34.8131658463958	37.0	37.0	37.0	27.0	37.0
40	34.79787408143633	37.0	37.0	37.0	27.0	37.0
41	34.78221309479617	37.0	37.0	37.0	27.0	37.0
42	34.631141553605	37.0	37.0	37.0	27.0	37.0
43	34.394087264047386	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	10.0
16	23.0
17	25.0
18	21.0
19	25.0
20	41.0
21	111.0
22	291.0
23	635.0
24	1291.0
25	2391.0
26	3965.0
27	6203.0
28	8629.0
29	12090.0
30	15733.0
31	20184.0
32	25823.0
33	33394.0
34	47066.0
35	85452.0
36	237650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88240389259441	20.307591596913706	14.095885872580599	22.71411863791128
2	16.032603272301987	23.916384261975754	38.97224650436879	21.078765961353465
3	17.178787116757874	29.318995557365074	30.999652732040854	22.502564593836194
4	11.222343300322919	18.47944532924595	41.43744985570418	28.860761514726956
5	13.016361509937052	37.56860537985926	34.81920112403054	14.595831986173147
6	26.121934961101996	43.18217198146307	16.588830744789984	14.107062312644944
7	25.10048817093567	33.76202964151568	23.149401062560123	17.988081124988526
8	24.180627237782755	34.50187005791791	20.329146159894943	20.988356544404397
9	27.10007304601899	13.972146714725358	20.416761466827925	38.511018772427725
10	15.927624567411897	27.386868481241542	32.899847122266266	23.7856598290803
11	33.44290236182128	24.787547849134025	23.439389766372486	18.330160022672207
12	24.00839031321973	27.562298674394377	28.508703652700106	19.920607359685782
13	28.29734918791188	23.533391610485097	26.00817476758992	22.1610844340131
14	22.675400256259802	21.615434663728863	29.5956124489576	26.11355263105374
15	24.592758465155452	27.81596394799762	27.43037676577774	20.160900821069188
16	20.96360871283335	29.172504360807416	26.324907095841965	23.53897983051727
17	21.62042414590044	26.274413536265552	26.564801398651642	25.540360919182366
18	23.17774132129471	25.619593896067087	29.6407173677887	21.5619474148495
19	26.00857392616365	24.69494306002946	29.515980313499146	19.78050270030775
20	24.683567040678252	24.71989047088737	30.06981283454478	20.5267296538896
21	22.049320033369657	27.150965764169133	29.907754453611783	20.891959748849427
22	21.80643204125703	26.850798516726744	29.887197787064867	21.455571654951363
23	21.558155408399095	26.497942337552438	30.972110790453723	20.97179146359474
24	22.026168836093515	27.297656540013655	28.54223297289314	22.13394165099969
25	21.843154630039876	26.897100911279026	30.667153640126614	20.592590818554488
26	21.97128453220611	27.266322591976117	29.752082609858416	21.010310265959358
27	22.179645307691388	26.77894997345596	29.4988164948289	21.54258822402376
28	20.88597237024353	26.60571515245862	30.732416066930913	21.775896410366947
29	23.345188342973014	26.523089327697214	28.987294782598283	21.14442754673149
30	22.869990060951515	26.000590754689117	30.247238820566245	20.882180363793125
31	21.995832784490293	26.747017287557828	29.759267464185495	21.49788246376638
32	20.19642593413085	26.20895153017439	30.579338753906764	23.01528378178799
33	20.173474316141572	26.242680429654293	31.624136320636097	21.959708933568038
34	21.5439852790318	26.13710298690361	30.944568848866588	21.374342885198004
35	21.518239551026436	27.33078670163296	29.634929568469666	21.516044178870942
36	22.44947650353056	27.200661006598093	29.20723115672163	21.14263133314972
37	21.5379979004259	26.648624699134228	29.499215653402626	22.314161747037247
38	21.766316604597506	26.665588938517605	29.170907726512514	22.397186730372376
39	20.970593987873563	26.714885022372837	29.87163060268953	22.442890387064068
40	20.99095107513362	27.75888427195472	30.477553317606485	20.77261133530518
41	21.72580200936426	25.912975447756132	29.708175166748497	22.653047376131116
42	20.531918715348045	27.94030184371345	29.68961429307021	21.838165147868295
43	21.92817540624364	25.803007260694454	29.575454940984404	22.6933623920775
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	91.0
1	102.0
2	113.0
3	420.0
4	727.0
5	727.0
6	929.0
7	1131.0
8	1113.5
9	1096.0
10	1458.0
11	1820.0
12	1820.0
13	2734.0
14	3648.0
15	6236.0
16	8824.0
17	9413.0
18	10002.0
19	10002.0
20	7888.0
21	5774.0
22	4694.5
23	3615.0
24	4339.0
25	5063.0
26	5063.0
27	6317.0
28	7571.0
29	9251.0
30	10931.0
31	14178.0
32	17425.0
33	17425.0
34	21513.0
35	25601.0
36	28092.0
37	30583.0
38	33189.0
39	35795.0
40	35795.0
41	37843.0
42	39891.0
43	41636.0
44	43381.0
45	42180.5
46	40980.0
47	40980.0
48	39853.5
49	38727.0
50	39231.5
51	39736.0
52	36853.0
53	33970.0
54	33970.0
55	32487.5
56	31005.0
57	25729.5
58	20454.0
59	18838.0
60	17222.0
61	17222.0
62	14086.0
63	10950.0
64	8164.5
65	5379.0
66	4693.5
67	4008.0
68	4008.0
69	3179.5
70	2351.0
71	1845.0
72	1339.0
73	1165.5
74	992.0
75	992.0
76	641.5
77	291.0
78	243.5
79	196.0
80	176.5
81	157.0
82	157.0
83	135.0
84	113.0
85	81.5
86	50.0
87	47.0
88	44.0
89	44.0
90	25.0
91	6.0
92	4.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	501054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.61776436779488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.61294523328434	33.061805458519025
2	6.37569453974751	4.924301388340115
3	2.00300560917455	2.3205479592742257
4	1.1342939353426509	1.75215583675525
5	0.7619283709673689	1.4711985147577826
6	0.5715289323989008	1.324270178445487
7	0.39020900207159065	1.054829950733515
8	0.3179986545753215	0.9824317689332449
9	0.2524893406046979	0.8775516474766913
>10	2.0227303736439066	15.99726965907946
>50	0.26995569974237116	7.286245875285655
>100	0.2642008301742196	20.043641326401364
>500	0.01935728854741807	5.01878975676494
>1k	0.0026158498037051445	1.4401164762376917
>5k	0.0010463399214820576	2.4448442029955535
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6934	1.383882775110068	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5167	1.0312261752226306	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2619	0.5226981522949622	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	0.27681647087938627	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1065	0.21255194050940618	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1056	0.21075572692763656	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1001	0.19977886615015547	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	911	0.18181673033245915	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	863	0.17223692456302114	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	852	0.17004155240752494	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	848	0.16924323526007176	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	825	0.16465291166221607	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	817	0.1630562773673097	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	813	0.16225796021985653	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	807	0.1610604844986768	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	793	0.15826637448259068	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	782	0.1560710023270945	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	782	0.1560710023270945	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	734	0.14649119655765647	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	730	0.14569287941020329	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	721	0.14389666582843366	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	717	0.1430983486809805	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	695	0.13870760436998805	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	657	0.13112359146918295	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	649	0.12952695717427665	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	637	0.1271320057319171	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	628	0.12533579215014748	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	623	0.12433789571583104	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	610	0.12174336498660823	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	602	0.12014673069170188	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	594	0.11855009639679555	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	590	0.11775177924934238	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	589	0.1175521999624791	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	577	0.11515724852011959	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	572	0.11415935208580312	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	571	0.11395977279893983	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	570	0.11376019351207653	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	554	0.11056692492226387	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	552	0.11016776634853728	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC	534	0.10657533918499802	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	522	0.10418038774263852	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	511	0.1019850155871423	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.10118669843968914	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	502	0.10018880200537267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.97896434316461E-4	0.0	0.0
2	5.987378605898765E-4	0.0	9.97896434316461E-4	0.0	0.0
3	5.987378605898765E-4	0.0	9.97896434316461E-4	0.0	0.0
4	5.987378605898765E-4	0.0	0.0017962135817696297	3.9915857372658435E-4	0.0
5	7.983171474531687E-4	0.0	0.002394951442359506	5.987378605898765E-4	0.0
6	7.983171474531687E-4	0.0	0.005987378605898765	5.987378605898765E-4	0.0
7	7.983171474531687E-4	0.0	0.019558770112602635	5.987378605898765E-4	0.0
8	7.983171474531687E-4	0.0	0.02275203870241531	5.987378605898765E-4	0.0
9	9.97896434316461E-4	0.0	0.02853983802145078	0.0013970550080430453	0.0
10	0.0027941100160860906	0.0	0.031733106611263454	0.0013970550080430453	0.0
11	0.0029936893029493825	0.0	0.051691035297592676	0.0013970550080430453	0.0
12	0.0029936893029493825	0.0	0.05568262103485852	0.0017962135817696297	0.0
13	0.0029936893029493825	0.0	0.05947462748526107	0.002195372155496214	0.0
14	0.0029936893029493825	0.0	0.06326663393566362	0.002195372155496214	0.0
15	0.0029936893029493825	0.0	0.06925401254156238	0.002195372155496214	0.0
16	0.0029936893029493825	0.0	0.07584012900805102	0.0025945307292227983	0.0
17	0.0029936893029493825	0.0	0.07763634258982066	0.0029936893029493825	0.0
18	0.0029936893029493825	0.0	0.08142834904022321	0.003193268589812675	0.0
19	0.0029936893029493825	0.0	0.08382330048258271	0.003193268589812675	0.0
20	0.0029936893029493825	0.0	0.08522035549062576	0.0035924271635392594	0.0
21	0.0029936893029493825	0.0	0.08761530693298526	0.004789902884719012	0.0
22	0.0029936893029493825	0.0	0.08921194122789161	0.005189061458445597	0.0
23	0.0029936893029493825	0.0	0.09060899623593464	0.006985275040215226	0.0
24	0.0029936893029493825	0.0	0.09060899623593464	0.009779385056301318	0.0
25	0.0029936893029493825	0.0	0.09080857552279795	0.010378122916891193	0.0
26	0.0029936893029493825	0.0	0.09120773409652452	0.010577702203754485	0.0
27	0.0029936893029493825	0.0	0.09120773409652452	0.01456928794102033	0.0
28	0.0029936893029493825	0.0	0.09140731338338783	0.032731003045579916	0.0
29	0.0029936893029493825	0.0	0.09140731338338783	0.084022879769446	0.0
30	0.0029936893029493825	0.0	0.09160689267025111	0.14689035513138304	0.0
31	0.0029936893029493825	0.0	0.09160689267025111	0.2808080566166521	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTACG	25	1.2326319E-4	37.0	16
GGTCGCT	25	0.005493421	29.6	28
GGTATCA	1130	0.0	26.849558	1
CGTTATT	35	8.8623865E-4	26.42857	10
GAGACGT	35	8.8623865E-4	26.42857	24
CCGCTCT	275	0.0	25.563635	28
GCCGCTC	275	0.0	25.563635	27
CCGGCAG	290	0.0	25.51724	16
GCCGGCA	290	0.0	25.51724	15
ACACGAT	45	1.321757E-4	24.666668	15
CACGATA	45	1.321757E-4	24.666668	16
CTAGTGC	45	1.321757E-4	24.666668	9
CGCTCTC	290	0.0	24.241379	29
TGCTCGC	315	0.0	24.079365	10
CGCCGTT	70	1.9184154E-7	23.785713	25
CGCCGGC	320	0.0	23.703125	14
GCAGCTT	305	0.0	23.655739	19
GTATCAA	3025	0.0	23.545456	1
GATTAAT	55	1.899883E-5	23.545454	1
TTCGCCG	300	0.0	23.433334	24
>>END_MODULE