FastQCFastQC Report
Fri 10 Feb 2017
ERR1633095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238240
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5670.23799529885829415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.15950302216252518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2470.10367696440564138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACC250.00548770829.64
CCGGTCT502.705492E-729.624
CGGTCTG509.051644E-625.90000225
GAAACCG601.3299832E-624.6666685
TCCGGTC601.3299832E-624.66666823
GTATCAA2950.023.830511
TACTCCG551.8944611E-523.54545420
TATTAGC400.001926720423.1252
AGCCTTA400.001926720423.1257
ATTACTC652.6680482E-622.7692318
AAACCGA652.6680482E-622.769236
GGACTTG502.6933974E-422.233
ATGGTAC502.6933974E-422.23
AACCGAC603.710346E-521.5833347
ACCGACC603.710346E-521.5833348
CCGACCT603.710346E-521.5833349
ACACGAT450.003817042620.55555715
CACGATA450.003817042620.55555716
TCTAGAC909.399264E-820.5555573
GGCCCGG450.003817042620.55555735
Produced by FastQC (version 0.11.5)