FastQCFastQC Report
Fri 10 Feb 2017
ERR1633094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372753
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9800.262908682156817No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7030.18859673832269627No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6950.18645054499896715No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6630.17786577170405068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.1754513042148554No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6150.16498861176167595No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5470.14674596850997845No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5220.14003911437332497No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4960.13306398607120531No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4670.12528403527268728No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4670.12528403527268728No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4480.12018682612883061No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4210.11294342366124485No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA4030.10811448868285434No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT3950.10596829535912522No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC3910.10489519869726065No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG3900.10462692453179451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3750.10060281204980241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG250.00549163929.67
GGTATCA1850.029.0000021
GTATCAA5450.024.4403671
GCTCGTG400.00192875323.12511
CGGCCTT753.7267455E-722.224
GGTTAGT450.003821032820.5555558
TACCCCG555.1341375E-420.1818185
TTCTAAG656.8861526E-519.9230772
TACGGGT759.238256E-619.73333415
TCGCCAT1103.8307917E-818.513
CCAATCA500.00702618518.529
ACGGGTC500.00702618518.516
GTAATAG500.00702618518.53
TTAAGGC500.00702618518.53
TCTAAGC609.220861E-418.53
GTACTAT500.00702618518.51
TTCCTCG902.1442884E-618.519
GCTTTTG701.216395E-418.51
GTCGCCA1156.370465E-817.69565212
TATTGTC852.715132E-517.4117645
Produced by FastQC (version 0.11.5)