##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633094.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372753
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75368139223561	33.0	33.0	33.0	27.0	33.0
2	31.29651002138145	33.0	33.0	33.0	27.0	33.0
3	31.717212202182143	33.0	33.0	33.0	27.0	33.0
4	31.843639085399715	33.0	33.0	33.0	27.0	33.0
5	31.962428203126468	33.0	33.0	33.0	33.0	33.0
6	34.87834034870276	37.0	37.0	37.0	27.0	37.0
7	35.00155330741805	37.0	37.0	37.0	33.0	37.0
8	35.19480728525324	37.0	37.0	37.0	33.0	37.0
9	35.2391583702881	37.0	37.0	37.0	33.0	37.0
10	35.311187300974105	37.0	37.0	37.0	33.0	37.0
11	35.35657660702932	37.0	37.0	37.0	33.0	37.0
12	35.27223389214842	37.0	37.0	37.0	33.0	37.0
13	35.32026301599182	37.0	37.0	37.0	33.0	37.0
14	35.24797922484863	37.0	37.0	37.0	33.0	37.0
15	35.30342076388386	37.0	37.0	37.0	33.0	37.0
16	35.283407511140084	37.0	37.0	37.0	33.0	37.0
17	35.285872950720716	37.0	37.0	37.0	33.0	37.0
18	35.25811998830325	37.0	37.0	37.0	33.0	37.0
19	35.29833965118993	37.0	37.0	37.0	33.0	37.0
20	35.22658167741105	37.0	37.0	37.0	33.0	37.0
21	35.268207096924776	37.0	37.0	37.0	33.0	37.0
22	35.30356831467486	37.0	37.0	37.0	33.0	37.0
23	35.29398019600111	37.0	37.0	37.0	33.0	37.0
24	35.2671286347796	37.0	37.0	37.0	33.0	37.0
25	35.264571981982705	37.0	37.0	37.0	33.0	37.0
26	35.23947225106169	37.0	37.0	37.0	33.0	37.0
27	35.160181138716524	37.0	37.0	37.0	33.0	37.0
28	35.18529428334581	37.0	37.0	37.0	33.0	37.0
29	35.22406526573897	37.0	37.0	37.0	33.0	37.0
30	35.20234578930284	37.0	37.0	37.0	33.0	37.0
31	35.17095502920164	37.0	37.0	37.0	33.0	37.0
32	35.206243812927056	37.0	37.0	37.0	33.0	37.0
33	35.16749429246713	37.0	37.0	37.0	33.0	37.0
34	35.136809093421114	37.0	37.0	37.0	33.0	37.0
35	35.13033295506676	37.0	37.0	37.0	33.0	37.0
36	35.10128154568843	37.0	37.0	37.0	33.0	37.0
37	35.15109737547384	37.0	37.0	37.0	33.0	37.0
38	34.98786864223762	37.0	37.0	37.0	27.0	37.0
39	35.06824089946962	37.0	37.0	37.0	27.0	37.0
40	35.11396554823167	37.0	37.0	37.0	33.0	37.0
41	35.13184065587668	37.0	37.0	37.0	33.0	37.0
42	35.02808562238265	37.0	37.0	37.0	27.0	37.0
43	34.831113364614104	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	17.0
17	17.0
18	9.0
19	16.0
20	23.0
21	67.0
22	203.0
23	442.0
24	916.0
25	1752.0
26	2816.0
27	4190.0
28	6063.0
29	8369.0
30	11001.0
31	14223.0
32	18379.0
33	24293.0
34	34722.0
35	64035.0
36	181190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.75755258844328	20.647452870935982	13.869506080433961	24.725488460186774
2	15.603093737676154	23.133549562310698	38.718400656735156	22.54495604327799
3	17.60307764122623	26.714741397118196	31.51738550729303	24.16479545436254
4	13.102510241366266	17.837280987678167	38.75032528242563	30.309883488529938
5	13.357370698559098	37.845168248142876	35.69763355358642	13.099827499711605
6	29.372533554391246	39.613363272730204	17.585103272139996	13.428999900738559
7	25.72722419403734	32.73883778266037	23.969760136068658	17.564177887233637
8	23.644075299192764	37.202651621851466	20.88728997486271	18.26598310409306
9	25.36022513567966	16.251512395607815	20.106343879190778	38.281918589521744
10	14.248309202072148	29.1748154944427	34.468669601586036	22.108205701899113
11	33.02025738223435	24.077069802255114	24.570425992547342	18.332246822963196
12	22.037649596381517	26.255456025840168	30.490700276054117	21.2161941017242
13	27.02647597738985	22.47600958275319	27.248606986395817	23.24890745346114
14	21.670113989692908	21.562536049340984	27.667114684522996	29.100235276443115
15	23.324292493957124	29.311903592995897	24.893696361934044	22.470107551112935
16	23.035897766081025	29.300367803880857	24.969349676595492	22.694384753442627
17	22.293046601905285	28.272877750145536	26.531778416270292	22.902297231678887
18	21.93329094601519	28.148130263203786	27.837468779593994	22.081110011187032
19	24.321735841160233	27.120640209468466	27.875027162759253	20.682596786612045
20	23.873181436500847	26.647672855751665	28.08079344767178	21.398352260075708
21	22.107937427733646	28.17039701893747	27.010111253296422	22.71155430003246
22	21.86461275965586	27.93914468830566	27.18395291251848	23.012289639520002
23	21.772058172570038	28.019626937945503	27.85651624534209	22.351798644142367
24	23.193642975375113	28.121571120822637	26.59857868347136	22.086207220330888
25	23.170571397145025	27.09005695460533	27.515539781034626	22.22383186721502
26	22.785597969701115	27.43720372471851	27.30601765780557	22.471180647774798
27	22.60800047216253	27.24565597057569	26.940091696109757	23.206251861152023
28	21.63497007401684	27.7494748533211	28.088305124304835	22.527249948357223
29	23.172181042137822	27.898367015154808	26.92801935866378	22.00143258404359
30	22.210954707272645	28.24095312445507	27.270605467964042	22.277486700308245
31	22.80410888711828	27.561414663329337	27.60058269148739	22.03389375806499
32	21.885806418727682	27.60728954562405	27.628751478861336	22.878152556786937
33	22.071452141230253	27.355111830085875	27.958460428219222	22.61497560046465
34	22.44757252121378	27.182879815856616	27.614801222257096	22.75474644067251
35	22.617390067953846	27.825664716313486	27.174295042561695	22.382650173170973
36	22.84703275359286	27.82807918380268	26.811588370851474	22.513299691752984
37	23.08901605084332	26.87651071889428	26.999112012512306	23.03536121775009
38	23.22529932690012	27.42218037145241	27.291799127035866	22.060721174611604
39	22.569637266500873	27.297969432841583	27.655310621242485	22.477082679415055
40	22.515982433407643	27.352965636762143	27.63921417131452	22.491837758515693
41	22.18841967737349	27.209438958237758	27.981800280614777	22.620341083773972
42	22.694653027608094	27.1734902200653	27.28723846622294	22.844618286103664
43	22.509275579270994	26.802467049225626	27.7843504948317	22.903906876671684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	13.5
2	17.0
3	53.0
4	89.0
5	89.0
6	120.0
7	151.0
8	136.5
9	122.0
10	203.5
11	285.0
12	285.0
13	407.0
14	529.0
15	1069.0
16	1609.0
17	1939.0
18	2269.0
19	2269.0
20	2328.0
21	2387.0
22	3092.0
23	3797.0
24	4831.0
25	5865.0
26	5865.0
27	7420.0
28	8975.0
29	11806.5
30	14638.0
31	15818.0
32	16998.0
33	16998.0
34	18669.5
35	20341.0
36	22011.5
37	23682.0
38	25810.0
39	27938.0
40	27938.0
41	29468.5
42	30999.0
43	29316.0
44	27633.0
45	27799.5
46	27966.0
47	27966.0
48	27347.0
49	26728.0
50	27411.0
51	28094.0
52	27946.5
53	27799.0
54	27799.0
55	23813.0
56	19827.0
57	17565.5
58	15304.0
59	13351.0
60	11398.0
61	11398.0
62	10354.0
63	9310.0
64	7736.0
65	6162.0
66	5247.5
67	4333.0
68	4333.0
69	3553.0
70	2773.0
71	2400.5
72	2028.0
73	1609.5
74	1191.0
75	1191.0
76	913.5
77	636.0
78	508.5
79	381.0
80	296.0
81	211.0
82	211.0
83	160.0
84	109.0
85	96.5
86	84.0
87	73.5
88	63.0
89	63.0
90	41.0
91	19.0
92	10.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	372753.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.96640943018156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.39609794905336	51.240044057452586
2	6.020878968294465	6.98017470811452
3	1.78849112962808	3.1101722724680756
4	0.8747807254734414	2.02831590777699
5	0.5332855375125085	1.5456323905322256
6	0.3463737496718077	1.2046825553605929
7	0.25501799543093767	1.0347734274649731
8	0.1940649993574331	0.899940096705671
9	0.17047772244935172	0.8893783312401577
>10	1.169822368288503	13.932950687567361
>50	0.1534188514693141	6.339073543645206
>100	0.09354808016421261	9.336441294928012
>500	0.0037419232065685044	1.4584207267436555
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	980	0.262908682156817	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	703	0.18859673832269627	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	695	0.18645054499896715	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	663	0.17786577170405068	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	654	0.1754513042148554	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	615	0.16498861176167595	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	547	0.14674596850997845	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	522	0.14003911437332497	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	496	0.13306398607120531	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	467	0.12528403527268728	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	467	0.12528403527268728	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	448	0.12018682612883061	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	421	0.11294342366124485	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	403	0.10811448868285434	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	395	0.10596829535912522	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	391	0.10489519869726065	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	390	0.10462692453179451	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.10060281204980241	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.682741654661398E-4	0.0
4	0.0	0.0	0.0	2.682741654661398E-4	0.0
5	0.0	0.0	0.0	2.682741654661398E-4	0.0
6	0.0	0.0	5.365483309322796E-4	2.682741654661398E-4	0.0
7	0.0	0.0	0.0021461933237291184	2.682741654661398E-4	0.0
8	0.0	0.0	0.0021461933237291184	2.682741654661398E-4	0.0
9	0.0	0.0	0.002414467489195258	0.0010730966618645592	0.0
10	0.0	0.0	0.0026827416546613978	0.0010730966618645592	0.0
11	0.0	0.0	0.0034875641510598173	0.0010730966618645592	0.0
12	0.0	0.0	0.0045606608129243765	0.0010730966618645592	0.0
13	0.0	0.0	0.0045606608129243765	0.0010730966618645592	0.0
14	0.0	0.0	0.005097209143856656	0.0010730966618645592	0.0
15	0.0	0.0	0.005902031640255075	0.0010730966618645592	0.0
16	0.0	0.0	0.005902031640255075	0.0010730966618645592	0.0
17	0.0	0.0	0.006170305805721215	0.0016096449927968386	0.0
18	0.0	0.0	0.006438579971187354	0.0016096449927968386	0.0
19	0.0	0.0	0.006975128302119635	0.0018779191582629785	0.0
20	0.0	0.0	0.007243402467585774	0.0018779191582629785	0.0
21	0.0	0.0	0.008048224963984192	0.0018779191582629785	0.0
22	0.0	0.0	0.008048224963984192	0.002414467489195258	0.0
23	0.0	0.0	0.008316499129450334	0.002414467489195258	0.0
24	0.0	0.0	0.008316499129450334	0.0026827416546613978	0.0
25	0.0	0.0	0.008316499129450334	0.0026827416546613978	0.0
26	0.0	0.0	0.008316499129450334	0.0034875641510598173	0.0
27	0.0	0.0	0.008316499129450334	0.0053654833093227956	0.0
28	0.0	0.0	0.008316499129450334	0.007779950798518054	0.0
29	0.0	0.0	0.008316499129450334	0.014218530769705409	0.0
30	0.0	0.0	0.008316499129450334	0.0276322390430124	0.0
31	0.0	0.0	0.008316499129450334	0.07297057300679002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGCG	25	0.005491639	29.6	7
GGTATCA	185	0.0	29.000002	1
GTATCAA	545	0.0	24.440367	1
GCTCGTG	40	0.001928753	23.125	11
CGGCCTT	75	3.7267455E-7	22.2	24
GGTTAGT	45	0.0038210328	20.555555	8
TACCCCG	55	5.1341375E-4	20.181818	5
TTCTAAG	65	6.8861526E-5	19.923077	2
TACGGGT	75	9.238256E-6	19.733334	15
TCGCCAT	110	3.8307917E-8	18.5	13
CCAATCA	50	0.007026185	18.5	29
ACGGGTC	50	0.007026185	18.5	16
GTAATAG	50	0.007026185	18.5	3
TTAAGGC	50	0.007026185	18.5	3
TCTAAGC	60	9.220861E-4	18.5	3
GTACTAT	50	0.007026185	18.5	1
TTCCTCG	90	2.1442884E-6	18.5	19
GCTTTTG	70	1.216395E-4	18.5	1
GTCGCCA	115	6.370465E-8	17.695652	12
TATTGTC	85	2.715132E-5	17.411764	5
>>END_MODULE