FastQCFastQC Report
Fri 10 Feb 2017
ERR1633093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403026
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39320.9756194389443856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32110.7967227920779304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15700.3895530313180787No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10250.25432602363122975No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8640.21437822869988538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.21040826150173936No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8460.2099120156019711No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7900.19601713040846No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7750.19229528616019811No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7520.1865884583128632No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6700.1662423764223648No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6180.15333998302839025No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5760.14291881913325694No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA5350.13274577818800773No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.117610278245076No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4680.11612154054577124No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4680.11612154054577124No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4670.11587341759588712No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4650.11537717169611886No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4530.11239969629750934No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4260.10570037665063792No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4050.10048979470307126No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4040.10024167175318716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTC308.298586E-637.08
CCCTAGG250.00549216129.61
AGCCAAT250.00549216129.619
ACCGTTC250.00549216129.68
GGTATCA6400.025.7265621
GTATCAA18050.024.290861
CATACTA1054.0017767E-1122.9047645
GCCGCTC2600.022.7692327
CGCTCTC2650.022.33962229
ACTGCTC3200.021.968758
GCCGGCA3000.021.58333415
CCGCTCT2750.021.52727328
TTCGCCG2850.021.42105324
GCCGTCC1850.021.027
GCTTCGC3100.020.88709622
CGCCGGC3100.020.88709614
CTTCGCC3050.020.62295223
CTAGTCC450.003821562520.5555559
CACCGTT450.003821562520.5555557
TCCCACA450.003821562520.5555552
Produced by FastQC (version 0.11.5)