##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	403026
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.755584006987142	33.0	33.0	33.0	27.0	33.0
2	31.248490171849955	33.0	33.0	33.0	27.0	33.0
3	31.698155950236462	33.0	33.0	33.0	27.0	33.0
4	31.81272424111596	33.0	33.0	33.0	27.0	33.0
5	31.93700406425392	33.0	33.0	33.0	33.0	33.0
6	34.796261283391146	37.0	37.0	37.0	27.0	37.0
7	34.98339561219375	37.0	37.0	37.0	27.0	37.0
8	35.17363147786991	37.0	37.0	37.0	33.0	37.0
9	35.27744363887194	37.0	37.0	37.0	33.0	37.0
10	35.287269307687346	37.0	37.0	37.0	33.0	37.0
11	35.33184459563403	37.0	37.0	37.0	33.0	37.0
12	35.26435267203605	37.0	37.0	37.0	33.0	37.0
13	35.31888761519108	37.0	37.0	37.0	33.0	37.0
14	35.263097169909635	37.0	37.0	37.0	33.0	37.0
15	35.29878221256197	37.0	37.0	37.0	33.0	37.0
16	35.30289360984155	37.0	37.0	37.0	33.0	37.0
17	35.26736736587714	37.0	37.0	37.0	33.0	37.0
18	35.2773121337085	37.0	37.0	37.0	33.0	37.0
19	35.26731277882816	37.0	37.0	37.0	33.0	37.0
20	35.268084441202305	37.0	37.0	37.0	33.0	37.0
21	35.27179636053257	37.0	37.0	37.0	33.0	37.0
22	35.27891997042374	37.0	37.0	37.0	33.0	37.0
23	35.2640201872832	37.0	37.0	37.0	33.0	37.0
24	35.24722226357605	37.0	37.0	37.0	33.0	37.0
25	35.25378759682998	37.0	37.0	37.0	33.0	37.0
26	35.21097150059798	37.0	37.0	37.0	33.0	37.0
27	35.152982685980554	37.0	37.0	37.0	33.0	37.0
28	35.12904874623474	37.0	37.0	37.0	33.0	37.0
29	35.15772679678234	37.0	37.0	37.0	33.0	37.0
30	35.11347903112951	37.0	37.0	37.0	33.0	37.0
31	35.10628098435337	37.0	37.0	37.0	33.0	37.0
32	35.12515321592155	37.0	37.0	37.0	33.0	37.0
33	35.08081364477726	37.0	37.0	37.0	33.0	37.0
34	35.00451335645839	37.0	37.0	37.0	27.0	37.0
35	35.000076918114466	37.0	37.0	37.0	27.0	37.0
36	34.96955035159022	37.0	37.0	37.0	27.0	37.0
37	34.97341362591992	37.0	37.0	37.0	27.0	37.0
38	34.811359564891596	37.0	37.0	37.0	27.0	37.0
39	34.85874608586047	37.0	37.0	37.0	27.0	37.0
40	34.87878201406361	37.0	37.0	37.0	27.0	37.0
41	34.84916357753594	37.0	37.0	37.0	27.0	37.0
42	34.74290740547756	37.0	37.0	37.0	27.0	37.0
43	34.48858386307583	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	30.0
17	24.0
18	21.0
19	19.0
20	34.0
21	85.0
22	200.0
23	520.0
24	1129.0
25	1928.0
26	3316.0
27	4930.0
28	7098.0
29	9407.0
30	12573.0
31	16137.0
32	20447.0
33	26942.0
34	37528.0
35	67999.0
36	192652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.404643869130034	18.719139708108163	14.208016356264858	23.66820006649695
2	16.213594160178253	24.09621215504707	37.87274270146343	21.81745098331125
3	19.083632321487944	26.86104618560589	30.64293618773975	23.412385305166417
4	13.097169909633621	18.00876370258991	37.995811684605954	30.898254703170515
5	15.091830303752115	36.2224769617841	32.79490653208478	15.890786202379005
6	28.57656826110474	39.161493303161585	17.158446353337006	15.103492082396668
7	25.967803566023033	32.96065266260737	21.5040716976076	19.56747207376199
8	25.226412191769263	34.00326529802047	20.046349367038356	20.723973143171904
9	27.376645675465106	14.482192215886815	18.62113114290393	39.52003096574415
10	16.158510865303978	27.408405413050275	31.614833782435873	24.818249939209878
11	34.93372636008595	23.865209688704947	22.686625676755344	18.514438274453756
12	23.668944435346603	25.261893773602694	29.111521341054917	21.957640449995782
13	30.367023467468602	21.698103844416984	25.595371018246958	22.339501669867452
14	23.330752854654538	21.060179740264896	27.723521559403114	27.885545845677452
15	25.535077141425116	26.879655406847203	24.93213837320669	22.65312907852099
16	22.415427292531994	27.592512641864296	24.965386848491164	25.026673217112545
17	23.054343888483622	25.788162550306925	25.41845935497958	25.73903420622987
18	24.894919930724072	24.116558236937568	27.433465830988574	23.55505600134979
19	27.154823758268698	24.65597752998566	27.48631601931389	20.702882692431753
20	26.61317135867165	23.472431059038374	27.295509470853	22.61888811143698
21	24.01582031928461	25.74027482097929	27.057311438964238	23.18659342077186
22	23.768193615300255	25.338811888066775	27.323051118290138	23.569943378342835
23	23.384595534779393	25.461880871209303	27.80837960826349	23.345143985747818
24	24.078347302655413	25.03312441380953	27.86817723918556	23.020351044349496
25	23.765712385801415	25.384714633795337	28.606094892140955	22.243478088262293
26	24.10464833534313	25.090688938182648	27.28881015120613	23.5158525752681
27	24.2207698758889	25.263382511302	27.728484018400795	22.7873635944083
28	23.058313855681767	25.85788509922437	27.108424766640365	23.9753762784535
29	23.526769985062998	25.877983058164983	26.620615047168172	23.974631909603847
30	23.993737376744924	25.814711705944532	27.7128522725581	22.47869864475245
31	23.998451712792722	25.111779388922802	27.226035044885442	23.663733853399034
32	22.44445767766844	25.49066313339586	27.947576583148482	24.11730260578722
33	22.981147618267805	25.28869105219018	28.010351689469164	23.719809640072846
34	22.497804111893526	25.633581952529116	27.58606144516731	24.282552490410048
35	22.9952906264112	25.83530591078491	27.432969585088802	23.736433877715086
36	23.09453980636485	25.435083592621815	28.12473636936575	23.345640231647586
37	24.441599301285773	25.37875968299812	27.134973922277965	23.04466709343814
38	23.515108206418446	25.77104206676492	26.930520611573446	23.783329115243184
39	23.240932346796484	24.942063291202057	27.68183690382258	24.135167458178877
40	23.302963084267518	25.515971674284042	27.950057812647323	23.23100742880112
41	23.614853632271863	25.307300273431494	27.77711611657809	23.30072997771856
42	23.191804002719426	25.867561894269848	27.508398961853576	23.432235141157147
43	23.02729848694625	25.81272672234546	27.36473577387067	23.795239016837623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	30.0
2	55.0
3	229.0
4	403.0
5	403.0
6	501.0
7	599.0
8	602.5
9	606.0
10	806.0
11	1006.0
12	1006.0
13	1650.0
14	2294.0
15	3972.0
16	5650.0
17	5872.0
18	6094.0
19	6094.0
20	4954.0
21	3814.0
22	3293.0
23	2772.0
24	3209.0
25	3646.0
26	3646.0
27	4539.5
28	5433.0
29	7102.5
30	8772.0
31	9525.5
32	10279.0
33	10279.0
34	11908.0
35	13537.0
36	14525.0
37	15513.0
38	17492.0
39	19471.0
40	19471.0
41	22448.5
42	25426.0
43	25409.0
44	25392.0
45	28356.0
46	31320.0
47	31320.0
48	33429.5
49	35539.0
50	36336.5
51	37134.0
52	38007.5
53	38881.0
54	38881.0
55	33729.5
56	28578.0
57	25935.5
58	23293.0
59	21063.0
60	18833.0
61	18833.0
62	16026.5
63	13220.0
64	10537.5
65	7855.0
66	7137.0
67	6419.0
68	6419.0
69	5341.5
70	4264.0
71	3570.5
72	2877.0
73	2472.5
74	2068.0
75	2068.0
76	1446.0
77	824.0
78	658.5
79	493.0
80	384.0
81	275.0
82	275.0
83	221.5
84	168.0
85	143.0
86	118.0
87	92.5
88	67.0
89	67.0
90	46.5
91	26.0
92	14.5
93	3.0
94	2.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	403026.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.48334077479498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48404472893635	46.254556358203146
2	7.661367000431377	8.195110041696804
3	1.9975342743752345	3.205044189172304
4	0.9180306176989	1.963973774723432
5	0.5415352824722804	1.4481558027019918
6	0.35774993043397846	1.1480196864935803
7	0.2518845854649099	0.9430140384236422
8	0.19024704025504743	0.8140037828285472
9	0.13685155741561544	0.6587350632738704
>10	1.159058748622176	12.710473237836966
>50	0.1836411858486223	6.849914861721795
>100	0.11149643426522772	11.54656265728709
>500	0.004684724128791081	1.8225348660946599
>1k	0.0018738896515164325	2.439901639542177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3932	0.9756194389443856	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3211	0.7967227920779304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1570	0.3895530313180787	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1025	0.25432602363122975	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	864	0.21437822869988538	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	848	0.21040826150173936	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	846	0.2099120156019711	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	790	0.19601713040846	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	775	0.19229528616019811	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	752	0.1865884583128632	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	670	0.1662423764223648	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	618	0.15333998302839025	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	576	0.14291881913325694	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	535	0.13274577818800773	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.117610278245076	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	468	0.11612154054577124	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	468	0.11612154054577124	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	467	0.11587341759588712	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	465	0.11537717169611886	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	453	0.11239969629750934	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	426	0.10570037665063792	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	405	0.10048979470307126	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	404	0.10024167175318716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.001736860649188886	0.0	0.0
2	0.0	2.481229498841266E-4	0.0019849835990730126	0.0	0.0
3	0.0	2.481229498841266E-4	0.0027293524487253923	0.0	0.0
4	0.0	2.481229498841266E-4	0.003473721298377772	7.443688496523797E-4	0.0
5	0.0	2.481229498841266E-4	0.005458704897450785	7.443688496523797E-4	0.0
6	0.0	2.481229498841266E-4	0.01240614749420633	7.443688496523797E-4	0.0
7	0.0	2.481229498841266E-4	0.027789770387022177	7.443688496523797E-4	0.0
8	0.0	2.481229498841266E-4	0.03200786053505233	7.443688496523797E-4	0.0
9	0.0	2.481229498841266E-4	0.039451549031576126	9.924917995365063E-4	0.0
10	0.0032255983484936455	2.481229498841266E-4	0.04491025392902691	9.924917995365063E-4	0.0
11	0.003473721298377772	2.481229498841266E-4	0.07121128661674433	9.924917995365063E-4	0.0
12	0.003473721298377772	2.481229498841266E-4	0.0774143603638475	9.924917995365063E-4	0.0
13	0.003473721298377772	2.481229498841266E-4	0.08510617181025541	0.0012406147494206329	0.0
14	0.003473721298377772	2.481229498841266E-4	0.09106112260747445	0.0012406147494206329	0.0
15	0.003473721298377772	2.481229498841266E-4	0.0990010570037665	0.0012406147494206329	0.0
16	0.003473721298377772	2.481229498841266E-4	0.10867785204924744	0.0012406147494206329	0.0
17	0.003473721298377772	2.481229498841266E-4	0.11066283564832045	0.0012406147494206329	0.0
18	0.003473721298377772	2.481229498841266E-4	0.11239969629750934	0.0014887376993047595	0.0
19	0.003473721298377772	2.481229498841266E-4	0.11463280284646649	0.0014887376993047595	0.0
20	0.003473721298377772	2.481229498841266E-4	0.11736215529519187	0.0019849835990730126	0.0
21	0.003473721298377772	2.481229498841266E-4	0.12083587659356965	0.0027293524487253923	0.0
22	0.003473721298377772	2.481229498841266E-4	0.12207649134299028	0.002977475398609519	0.0
23	0.003473721298377772	2.481229498841266E-4	0.12257273724275854	0.004218090148030152	0.0
24	0.003473721298377772	2.481229498841266E-4	0.12257273724275854	0.004714336047798405	0.0
25	0.003473721298377772	2.481229498841266E-4	0.12257273724275854	0.004714336047798405	0.0
26	0.003473721298377772	2.481229498841266E-4	0.12257273724275854	0.0049624589976825315	0.0
27	0.003473721298377772	2.481229498841266E-4	0.12257273724275854	0.00793993439629205	0.0
28	0.003473721298377772	2.481229498841266E-4	0.12282086019264266	0.02257918843945552	0.0
29	0.0037218442482618986	2.481229498841266E-4	0.12282086019264266	0.05954950797219038	0.0
30	0.0037218442482618986	2.481229498841266E-4	0.12306898314252679	0.10098604060283951	0.0
31	0.0037218442482618986	2.481229498841266E-4	0.12306898314252679	0.21239324510081237	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGTC	30	8.298586E-6	37.0	8
CCCTAGG	25	0.005492161	29.6	1
AGCCAAT	25	0.005492161	29.6	19
ACCGTTC	25	0.005492161	29.6	8
GGTATCA	640	0.0	25.726562	1
GTATCAA	1805	0.0	24.29086	1
CATACTA	105	4.0017767E-11	22.904764	5
GCCGCTC	260	0.0	22.76923	27
CGCTCTC	265	0.0	22.339622	29
ACTGCTC	320	0.0	21.96875	8
GCCGGCA	300	0.0	21.583334	15
CCGCTCT	275	0.0	21.527273	28
TTCGCCG	285	0.0	21.421053	24
GCCGTCC	185	0.0	21.0	27
GCTTCGC	310	0.0	20.887096	22
CGCCGGC	310	0.0	20.887096	14
CTTCGCC	305	0.0	20.622952	23
CTAGTCC	45	0.0038215625	20.555555	9
CACCGTT	45	0.0038215625	20.555555	7
TCCCACA	45	0.0038215625	20.555555	2
>>END_MODULE