FastQCFastQC Report
Fri 10 Feb 2017
ERR1633092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385786
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7520.19492672103186742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.1443805633174869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC250.005491872329.615
GGTATCA1900.029.2105271
TAACGGC702.055458E-1029.07142836
TTAACGG702.055458E-1029.07142835
CGCTCTC358.8586734E-426.4285729
TTTAACG809.731593E-1025.437534
AACGGCC809.731593E-1025.437537
GTATAGC451.3209869E-424.6666661
CCGCTCT400.001928874623.12528
TATTGTC802.7139322E-823.1255
ACAGCGT400.001928874623.1258
ATTAGAG1107.4578566E-1121.8636363
CACCGCC957.1086106E-921.42105311
GTATCAA4500.020.5555551
GAATTAG450.003821270520.5555551
TTCGCCG450.003821270520.55555524
GCTCGCC450.003821270520.55555511
TCGGTTT450.003821270520.55555514
GCACCGC1001.2831151E-820.3510
GCCGCTC555.1345845E-420.18181827
Produced by FastQC (version 0.11.5)