FastQCFastQC Report
Fri 10 Feb 2017
ERR1633091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422177
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8960.21223325761469714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6390.15135831653548154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCG250.00549245329.69
GTATCAA4700.025.5851041
GGTATCA1200.024.6666661
CCGTTAA502.6981792E-422.216
GCCGAGT450.003821859120.55555512
ATACGGA450.003821859120.5555554
CGTTAAA555.135687E-420.18181817
CAAACGC500.0070276918.530
TATTAGC801.6127247E-518.52
GCTCCTA1002.8642353E-718.511
ATCAACG6800.017.4117643
TCAACGC6850.017.2846724
GCCGTTA650.001577696417.07692315
TCTATAC1107.7788536E-716.8181823
CAACGCA7100.016.6760565
AACGCAG7050.016.5319166
TACGGGT1352.2075255E-816.44444515
TAACCTG904.4352448E-516.4444455
CTAGACA803.3762268E-416.18754
GGTACCG803.3762268E-416.18756
Produced by FastQC (version 0.11.5)