##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633091.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422177
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.733178264093024	33.0	33.0	33.0	27.0	33.0
2	31.276268010810632	33.0	33.0	33.0	27.0	33.0
3	31.694696774101857	33.0	33.0	33.0	27.0	33.0
4	31.833039222885187	33.0	33.0	33.0	27.0	33.0
5	31.925869955018868	33.0	33.0	33.0	33.0	33.0
6	34.83426145905627	37.0	37.0	37.0	27.0	37.0
7	34.97648853442987	37.0	37.0	37.0	27.0	37.0
8	35.15876279380449	37.0	37.0	37.0	33.0	37.0
9	35.20322518753982	37.0	37.0	37.0	33.0	37.0
10	35.27435175293775	37.0	37.0	37.0	33.0	37.0
11	35.3083469729521	37.0	37.0	37.0	33.0	37.0
12	35.2273951446905	37.0	37.0	37.0	33.0	37.0
13	35.28619749536332	37.0	37.0	37.0	33.0	37.0
14	35.239259836514066	37.0	37.0	37.0	33.0	37.0
15	35.26695201301823	37.0	37.0	37.0	33.0	37.0
16	35.2699578612762	37.0	37.0	37.0	33.0	37.0
17	35.23810392323599	37.0	37.0	37.0	33.0	37.0
18	35.2165300336115	37.0	37.0	37.0	33.0	37.0
19	35.24692723668035	37.0	37.0	37.0	33.0	37.0
20	35.19618785485709	37.0	37.0	37.0	33.0	37.0
21	35.26058027794029	37.0	37.0	37.0	33.0	37.0
22	35.26829031425208	37.0	37.0	37.0	33.0	37.0
23	35.227409356738995	37.0	37.0	37.0	33.0	37.0
24	35.22398188437551	37.0	37.0	37.0	33.0	37.0
25	35.23998701966237	37.0	37.0	37.0	33.0	37.0
26	35.20616471290478	37.0	37.0	37.0	33.0	37.0
27	35.13664174031271	37.0	37.0	37.0	33.0	37.0
28	35.14270317899838	37.0	37.0	37.0	33.0	37.0
29	35.16491187345592	37.0	37.0	37.0	33.0	37.0
30	35.1271457232393	37.0	37.0	37.0	33.0	37.0
31	35.131456711284606	37.0	37.0	37.0	33.0	37.0
32	35.16000161069883	37.0	37.0	37.0	33.0	37.0
33	35.14175097174881	37.0	37.0	37.0	33.0	37.0
34	35.093465536966725	37.0	37.0	37.0	33.0	37.0
35	35.07467957752317	37.0	37.0	37.0	33.0	37.0
36	35.06247853388508	37.0	37.0	37.0	33.0	37.0
37	35.07476248113943	37.0	37.0	37.0	33.0	37.0
38	34.946110280759015	37.0	37.0	37.0	27.0	37.0
39	34.99864511804291	37.0	37.0	37.0	27.0	37.0
40	35.05427818190001	37.0	37.0	37.0	27.0	37.0
41	35.082074580093185	37.0	37.0	37.0	33.0	37.0
42	34.95137584472863	37.0	37.0	37.0	27.0	37.0
43	34.78352918325726	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	10.0
16	25.0
17	24.0
18	15.0
19	21.0
20	33.0
21	96.0
22	253.0
23	557.0
24	1141.0
25	2095.0
26	3345.0
27	5089.0
28	7201.0
29	9703.0
30	12725.0
31	16581.0
32	20849.0
33	27308.0
34	39453.0
35	72376.0
36	203275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.451043045926234	20.585204783775527	13.592166318866258	25.371585851431984
2	15.86325166932353	22.902479291860995	37.71640804686186	23.51786099195361
3	17.77690399998105	26.680515518372626	30.145412942912568	25.397167538733754
4	12.949071124196724	17.916655810240727	38.77804806988538	30.356224995677167
5	14.087456209125557	36.934745379307735	35.45408679298019	13.523711618586518
6	30.080037519808045	39.52536495356213	17.35622736435192	13.038370162277907
7	26.710834555174724	31.88709948670818	23.89211160247953	17.509954355637564
8	24.423879083891354	36.276253798762134	20.680188641257104	18.619678476089412
9	25.784208992910557	15.638938170482996	20.257380198352823	38.31947263825362
10	14.765844657572536	28.250473142781345	34.42418701160888	22.559495188037246
11	34.26027471889753	23.384741470994395	24.250728959654364	18.104254850453717
12	22.979224353766313	25.37205958638202	30.4924237938116	21.156292266040072
13	28.059557957918123	21.987223368397615	26.06844996293024	23.88476871075402
14	22.012805055699385	20.52646164997146	27.57232156180938	29.88841173251977
15	23.74785931019454	28.846668577397633	23.9494335314335	23.45603858097433
16	23.2606229140858	28.206415792428295	25.04636680823446	23.486594485251448
17	22.47161735480616	27.51144662073017	26.608034071017606	23.408901953446065
18	23.331446289115704	27.33616468921803	27.15188179365527	22.180507228011002
19	24.416536192165843	26.641432384994918	26.934911186540244	22.007120236298995
20	23.98093690561068	26.641669252469935	26.983232151443588	22.394161690475794
21	23.175350623079893	27.089822515200968	27.012603718345623	22.722223143373512
22	23.03015086089484	26.72125672407545	26.996022995094478	23.25256941993524
23	22.94890531696421	26.690937687273347	27.30063456796557	23.05952242779687
24	23.003858571168017	27.197360348858417	26.35648081255019	23.44230026742338
25	23.484699545451313	26.641432384994918	27.069688779824574	22.80417928972919
26	23.320076650314917	26.97920540436831	26.941069740890665	22.759648204426107
27	23.064496644772216	27.065662032749298	26.509260333935764	23.36058098854272
28	23.098131826224545	26.66298732522141	27.217257216759794	23.021623631794245
29	23.721330152992703	26.736416242476498	26.66961961452187	22.872633990008932
30	23.395163639895113	26.80463407528122	26.81789865388214	22.982303630941527
31	23.44135279752331	26.667487807246726	26.901986607512963	22.989172787717
32	22.78333495192775	26.61016587829275	27.0455282973729	23.560970872406596
33	22.687877359496138	26.712966362449876	27.41338348607338	23.185772791980614
34	23.44561641207361	26.385615511977207	27.131984925753887	23.036783150195298
35	23.110448934925397	26.92448901763952	26.91998853561421	23.04507351182087
36	23.819393287649493	26.76507720695348	26.518735032936426	22.896794472460602
37	23.11684435675084	26.347953583449595	26.92662082491467	23.608581234884895
38	23.176534960454976	26.891564438612242	26.7494439536024	23.182456647330383
39	22.777650132527352	26.785210942329872	27.177700348432055	23.259438576710718
40	23.047915921521067	26.878062992536307	27.42641119719928	22.647609888743347
41	23.07160266902271	26.383483704702055	27.054529261423525	23.49038436485171
42	22.80749543437942	27.0483707070731	27.143591432029694	23.000542426517786
43	23.200221707956615	26.22454562896605	27.23525914486104	23.339973518216294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	8.0
2	12.0
3	58.5
4	105.0
5	105.0
6	115.5
7	126.0
8	121.0
9	116.0
10	172.0
11	228.0
12	228.0
13	367.5
14	507.0
15	918.5
16	1330.0
17	1578.5
18	1827.0
19	1827.0
20	1884.5
21	1942.0
22	2352.0
23	2762.0
24	3877.0
25	4992.0
26	4992.0
27	6781.0
28	8570.0
29	10358.0
30	12146.0
31	14550.0
32	16954.0
33	16954.0
34	19750.0
35	22546.0
36	24722.5
37	26899.0
38	29194.5
39	31490.0
40	31490.0
41	32760.0
42	34030.0
43	34322.5
44	34615.0
45	34212.0
46	33809.0
47	33809.0
48	32636.0
49	31463.0
50	30953.0
51	30443.0
52	28835.5
53	27228.0
54	27228.0
55	25936.0
56	24644.0
57	22023.5
58	19403.0
59	17964.5
60	16526.0
61	16526.0
62	14620.0
63	12714.0
64	10614.5
65	8515.0
66	7274.5
67	6034.0
68	6034.0
69	5006.0
70	3978.0
71	3272.5
72	2567.0
73	2080.0
74	1593.0
75	1593.0
76	1220.5
77	848.0
78	707.5
79	567.0
80	427.0
81	287.0
82	287.0
83	229.0
84	171.0
85	136.5
86	102.0
87	78.5
88	55.0
89	55.0
90	39.0
91	23.0
92	13.5
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	422177.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.87846682859794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.53926116040692	65.25484074335883
2	6.308235651047042	9.194690852836215
3	1.6333636225837493	3.5711110976252494
4	0.7232321753529366	2.1083220840333485
5	0.4098560447413249	1.4934840080590495
6	0.25200582584049014	1.101947893147775
7	0.17167026219144801	0.8757745856003307
8	0.13523040565539135	0.7884307706219423
9	0.10256551266733967	0.6727335581416368
>10	0.6425345333654299	9.116589342668014
>50	0.05883691282972764	2.865299466474001
>100	0.022554149844461455	2.5911092630748764
>500	6.537434737525059E-4	0.3656663343587327
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	896	0.21223325761469714	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	639	0.15135831653548154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	9.474699000656123E-4	0.0	0.0
7	0.0	0.0	0.002842409700196837	0.0	0.0
8	0.0	0.0	0.003789879600262449	0.0	0.0
9	0.0	0.0	0.004263614550295255	4.7373495003280613E-4	0.0
10	0.0	0.0	0.005684819400393674	4.7373495003280613E-4	0.0
11	0.0	0.0	0.008764096575606914	4.7373495003280613E-4	0.0
12	0.0	0.0	0.009948433950688928	4.7373495003280613E-4	0.0
13	0.0	0.0	0.010422168900721736	4.7373495003280613E-4	0.0
14	0.0	0.0	0.010659036375738139	4.7373495003280613E-4	0.0
15	0.0	0.0	0.011843373750820153	4.7373495003280613E-4	0.0
16	0.0	0.0	0.01231710870085296	7.106024250492092E-4	0.0
17	0.0	0.0	0.01302771112590217	7.106024250492092E-4	0.0
18	0.0	0.0	0.013738313550951379	7.106024250492092E-4	0.0
19	0.0	0.0	0.014212048500984184	7.106024250492092E-4	0.0
20	0.0	0.0	0.01468578345101699	7.106024250492092E-4	0.0
21	0.0	0.0	0.01468578345101699	7.106024250492092E-4	0.0
22	0.0	0.0	0.014922650926033393	9.474699000656123E-4	0.0
23	0.0	0.0	0.014922650926033393	0.0011843373750820153	0.0
24	0.0	0.0	0.014922650926033393	0.0014212048500984184	0.0
25	0.0	0.0	0.015159518401049796	0.0016580723251148216	0.0
26	0.0	0.0	0.015159518401049796	0.0018949398001312245	0.0
27	0.0	0.0	0.015159518401049796	0.002842409700196837	0.0
28	0.0	0.0	0.015159518401049796	0.006869156775475689	0.0
29	0.0	0.0	0.015159518401049796	0.01610698830111541	0.0
30	0.0	0.0	0.015159518401049796	0.03647759115252607	0.0
31	0.0	0.0	0.015159518401049796	0.08835156818111835	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTCG	25	0.005492453	29.6	9
GTATCAA	470	0.0	25.585104	1
GGTATCA	120	0.0	24.666666	1
CCGTTAA	50	2.6981792E-4	22.2	16
GCCGAGT	45	0.0038218591	20.555555	12
ATACGGA	45	0.0038218591	20.555555	4
CGTTAAA	55	5.135687E-4	20.181818	17
CAAACGC	50	0.00702769	18.5	30
TATTAGC	80	1.6127247E-5	18.5	2
GCTCCTA	100	2.8642353E-7	18.5	11
ATCAACG	680	0.0	17.411764	3
TCAACGC	685	0.0	17.284672	4
GCCGTTA	65	0.0015776964	17.076923	15
TCTATAC	110	7.7788536E-7	16.818182	3
CAACGCA	710	0.0	16.676056	5
AACGCAG	705	0.0	16.531916	6
TACGGGT	135	2.2075255E-8	16.444445	15
TAACCTG	90	4.4352448E-5	16.444445	5
CTAGACA	80	3.3762268E-4	16.1875	4
GGTACCG	80	3.3762268E-4	16.1875	6
>>END_MODULE