FastQCFastQC Report
Fri 10 Feb 2017
ERR1633090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455444
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24300.5335452876753234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.39477959968733806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9570.21012462564003478No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5170.11351560235726017No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5080.11153950869920341No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.10934384907914035No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4910.1078068873450962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCG250.005492903329.59999825
TTAACGG751.3691533E-824.66666635
GTATCAA13750.024.3527281
GGTATCA4800.024.2812481
AGACGTA400.001929406723.12524
TATTAGA1107.4578566E-1121.8636362
GCACCGC957.1158865E-921.42105310
CACCGCC957.1158865E-921.42105311
ATTAGAG909.4467396E-820.5555573
GTATACA450.00382231420.5555571
TAACGGC1001.2845703E-820.34999836
CTACCGA555.136541E-420.18181825
CGAGCCT555.136541E-420.18181829
TACCGAG555.136541E-420.18181826
ATATACC656.890442E-519.9230774
ATGCCCG759.245641E-619.73333429
AACGGCC951.6705599E-719.47368437
TTCGCCG1355.4569682E-1119.18518624
CGGTTGG609.225149E-418.533
GCCGGCA1409.276846E-1118.515
Produced by FastQC (version 0.11.5)