##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633090.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455444
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76459454949456	33.0	33.0	33.0	27.0	33.0
2	31.282840480937285	33.0	33.0	33.0	27.0	33.0
3	31.716459103643917	33.0	33.0	33.0	27.0	33.0
4	31.84088493865327	33.0	33.0	33.0	27.0	33.0
5	31.951282704350042	33.0	33.0	33.0	33.0	33.0
6	34.84315525070041	37.0	37.0	37.0	27.0	37.0
7	35.018851933497864	37.0	37.0	37.0	33.0	37.0
8	35.189184619843495	37.0	37.0	37.0	33.0	37.0
9	35.27680680830135	37.0	37.0	37.0	33.0	37.0
10	35.315975180263656	37.0	37.0	37.0	33.0	37.0
11	35.335114745171744	37.0	37.0	37.0	33.0	37.0
12	35.29966142928659	37.0	37.0	37.0	33.0	37.0
13	35.32189687425897	37.0	37.0	37.0	33.0	37.0
14	35.278115421434904	37.0	37.0	37.0	33.0	37.0
15	35.309678028473314	37.0	37.0	37.0	33.0	37.0
16	35.31032794372085	37.0	37.0	37.0	33.0	37.0
17	35.27474508391811	37.0	37.0	37.0	33.0	37.0
18	35.28508664072861	37.0	37.0	37.0	33.0	37.0
19	35.27254722863843	37.0	37.0	37.0	33.0	37.0
20	35.280936844046686	37.0	37.0	37.0	33.0	37.0
21	35.283255460605474	37.0	37.0	37.0	33.0	37.0
22	35.289113480471805	37.0	37.0	37.0	33.0	37.0
23	35.27153063823434	37.0	37.0	37.0	33.0	37.0
24	35.27128911567613	37.0	37.0	37.0	33.0	37.0
25	35.27460895302167	37.0	37.0	37.0	33.0	37.0
26	35.2311107402886	37.0	37.0	37.0	33.0	37.0
27	35.19080282098348	37.0	37.0	37.0	33.0	37.0
28	35.13891060152291	37.0	37.0	37.0	33.0	37.0
29	35.178010908036995	37.0	37.0	37.0	33.0	37.0
30	35.141841807115696	37.0	37.0	37.0	33.0	37.0
31	35.13675226811639	37.0	37.0	37.0	33.0	37.0
32	35.162105549749256	37.0	37.0	37.0	33.0	37.0
33	35.1343568034709	37.0	37.0	37.0	33.0	37.0
34	35.07097689287816	37.0	37.0	37.0	33.0	37.0
35	35.05018399627616	37.0	37.0	37.0	27.0	37.0
36	35.050072017635536	37.0	37.0	37.0	27.0	37.0
37	35.05472242471083	37.0	37.0	37.0	27.0	37.0
38	34.909800107148186	37.0	37.0	37.0	27.0	37.0
39	34.97492117581964	37.0	37.0	37.0	27.0	37.0
40	35.007550873433395	37.0	37.0	37.0	27.0	37.0
41	35.02125837644145	37.0	37.0	37.0	27.0	37.0
42	34.915467104627574	37.0	37.0	37.0	27.0	37.0
43	34.70584967635977	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	14.0
16	26.0
17	30.0
18	24.0
19	16.0
20	26.0
21	93.0
22	207.0
23	561.0
24	1144.0
25	2122.0
26	3595.0
27	5501.0
28	7716.0
29	10687.0
30	13729.0
31	17729.0
32	22521.0
33	29429.0
34	41811.0
35	76634.0
36	221827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.194307093737095	20.139907430990416	13.863833973002167	24.80195150227031
2	15.927973581823451	23.277065896136516	38.14936633263365	22.64559418940638
3	17.983769684088493	26.8941955542284	30.436892351200147	24.685142410482957
4	12.607697104364094	18.057543847322613	39.419994554764145	29.91476449354915
5	14.259272270575526	36.524797779748994	35.01528179095564	14.200648158719842
6	29.16692282695567	40.062005427670584	17.215947514952443	13.555124230421303
7	26.17709312231581	32.50871676869165	23.33525087606819	17.978939232924354
8	24.449987265174205	35.267563081300885	20.78718788698501	19.495261766539905
9	26.489974618174795	15.025337912015527	20.143420486382517	38.34126698342716
10	15.181668876964016	27.87016625534643	33.73455353457286	23.213611333116695
11	34.215402991366666	23.597193068741714	23.961233433748166	18.226170506143454
12	23.293972475211003	25.709417623242377	29.97712122675894	21.019488674787677
13	28.59714915554931	22.069892237025847	26.041620923757915	23.291337683666928
14	22.31931916986501	21.083821501655528	27.87499670651057	28.721862621968892
15	24.149181897225564	28.168556397713	24.5927051404783	23.089556564583134
16	22.628248478407883	28.242550126909126	25.384240433511035	23.744960961171955
17	22.481358849825664	26.95655228743819	26.59844020340591	23.963648659330236
18	23.895802777070287	26.086192814045194	27.658065536048337	22.359938872836178
19	25.376336058878806	25.90944221463012	27.270751179069215	21.443470547421857
20	24.67943369547079	25.78231352262847	27.31993395455863	22.21831882734211
21	23.531103714177814	26.432228770167132	27.474069259887056	22.562598255768
22	23.13303062506038	26.354063287692885	27.446623514636265	23.066282572610465
23	22.910610305547994	26.494146371452914	27.9002467921413	22.6949965308578
24	23.241715776253503	26.46867671986018	26.949745742616	23.33986176127032
25	23.4017793625561	26.401928667410264	27.96545788285717	22.230834087176472
26	23.409683737188324	26.574068381623206	27.44398872309219	22.572259158096276
27	23.262135410720088	26.59536627993782	27.27536206427135	22.867136245070743
28	22.767233732357877	26.60832067169619	27.681119962059004	22.943325633886932
29	23.511342777597246	26.583729283951484	26.858406302421372	23.046521636029897
30	23.44920561034946	26.45396580040576	27.540817312336973	22.55601127690781
31	23.564258174440766	26.230008519159327	27.156357312863932	23.04937599353598
32	22.3781628476827	26.226495463767225	27.767189819165473	23.6281518693846
33	22.564574349426056	26.374922054083488	28.074362600012297	22.986140996478163
34	22.945082161582985	26.136034287420628	27.695830881513423	23.223052669482964
35	22.8794319389431	26.785290837073273	27.437401744232005	22.89787547975163
36	23.61344094993018	26.338034972466428	27.33486443997506	22.713659637628336
37	23.46062304037379	26.030862191619608	27.354186244631613	23.154328523374993
38	23.09482614767128	26.5529900492706	27.14735510842167	23.204828694636443
39	22.76679460043386	26.311028359139655	27.551136912551264	23.37104012787522
40	22.621002801661675	26.79495173940155	27.97336225748939	22.61068320144738
41	23.09790007113937	26.139327776850724	27.67958300032496	23.083189151684948
42	22.613317992991455	26.89661077981047	27.534230333476785	22.95584089372129
43	23.108878369239687	26.05018399627616	27.74128103564873	23.099656598835423
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	20.5
2	35.0
3	143.0
4	251.0
5	251.0
6	320.0
7	389.0
8	381.5
9	374.0
10	494.0
11	614.0
12	614.0
13	984.5
14	1355.0
15	2278.0
16	3201.0
17	3573.5
18	3946.0
19	3946.0
20	3482.5
21	3019.0
22	3137.5
23	3256.0
24	4318.0
25	5380.0
26	5380.0
27	6872.0
28	8364.0
29	10429.0
30	12494.0
31	14596.5
32	16699.0
33	16699.0
34	19584.5
35	22470.0
36	24441.5
37	26413.0
38	28456.5
39	30500.0
40	30500.0
41	32292.0
42	34084.0
43	35152.0
44	36220.0
45	36211.5
46	36203.0
47	36203.0
48	35739.0
49	35275.0
50	35288.5
51	35302.0
52	34001.0
53	32700.0
54	32700.0
55	30256.0
56	27812.0
57	24649.0
58	21486.0
59	19836.0
60	18186.0
61	18186.0
62	15638.0
63	13090.0
64	10938.5
65	8787.0
66	7701.5
67	6616.0
68	6616.0
69	5528.5
70	4441.0
71	3583.0
72	2725.0
73	2189.0
74	1653.0
75	1653.0
76	1266.0
77	879.0
78	699.0
79	519.0
80	400.5
81	282.0
82	282.0
83	231.5
84	181.0
85	152.5
86	124.0
87	101.0
88	78.0
89	78.0
90	51.5
91	25.0
92	17.0
93	9.0
94	5.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	455444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.19165029978366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.90837782631952	54.918838197222556
2	7.3381474553557275	9.274192956941729
3	2.0368080133368114	3.861277791197306
4	0.9800815338210839	2.4773187820199007
5	0.580181749627342	1.8331321116383816
6	0.37491562992425154	1.4214922426857846
7	0.2691672954644354	1.1906387924989004
8	0.1987979167550565	1.0049894748728827
9	0.1485171179807475	0.8446537604673997
>10	1.0210775059026096	12.590321382197939
>50	0.09257501997059737	3.955903083056927
>100	0.04960623705401652	5.256362065735458
>500	0.0010480190926904897	0.4375334767245256
>1k	6.986793951269932E-4	0.9333458827403098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2430	0.5335452876753234	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1798	0.39477959968733806	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	957	0.21012462564003478	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	517	0.11351560235726017	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	508	0.11153950869920341	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	498	0.10934384907914035	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	491	0.1078068873450962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.586978860189178E-4	0.0	0.0
2	2.1956596200630593E-4	0.0	8.782638480252237E-4	0.0	0.0
3	2.1956596200630593E-4	0.0	0.0013173957720378356	0.0	0.0
4	2.1956596200630593E-4	0.0	0.0021956596200630595	2.1956596200630593E-4	0.0
5	2.1956596200630593E-4	0.0	0.004391319240126119	4.3913192401261186E-4	0.0
6	2.1956596200630593E-4	0.0	0.009002204442258544	4.3913192401261186E-4	0.0
7	2.1956596200630593E-4	0.0	0.015150051378435109	6.586978860189178E-4	0.0
8	2.1956596200630593E-4	0.0	0.01888267273254231	6.586978860189178E-4	0.0
9	2.1956596200630593E-4	0.0	0.022834860048655817	0.0010978298100315297	0.0
10	0.0019760936580567536	0.0	0.02546965159273149	0.0010978298100315297	0.0
11	0.0019760936580567536	0.0	0.03666751565505309	0.0010978298100315297	0.0
12	0.0019760936580567536	0.0	0.03952187316113507	0.0010978298100315297	0.0
13	0.0021956596200630595	0.0	0.04479145624928641	0.0010978298100315297	0.0
14	0.0021956596200630595	0.0	0.04918277548941253	0.0013173957720378356	0.0
15	0.0021956596200630595	0.0	0.05379366069154495	0.0013173957720378356	0.0
16	0.0021956596200630595	0.0	0.05884367781768999	0.0013173957720378356	0.0
17	0.0021956596200630595	0.0	0.05994150762772152	0.0013173957720378356	0.0
18	0.0021956596200630595	0.0	0.061258903399759354	0.0015369617340441415	0.0
19	0.0021956596200630595	0.0	0.0627958651338035	0.0015369617340441415	0.0
20	0.0021956596200630595	0.0	0.06455239282985395	0.0017565276960504474	0.0
21	0.0021956596200630595	0.0	0.06762631629794223	0.0019760936580567536	0.0
22	0.0021956596200630595	0.0	0.06850458014596746	0.0024152255820693654	0.0
23	0.0021956596200630595	0.0	0.06872414610797375	0.002854357506081977	0.0
24	0.0021956596200630595	0.0	0.06894371206998007	0.002854357506081977	0.0
25	0.0021956596200630595	0.0	0.06894371206998007	0.003293489430094589	0.0
26	0.0021956596200630595	0.0	0.06894371206998007	0.003952187316113507	0.0
27	0.0021956596200630595	0.0	0.06894371206998007	0.004610885202132425	0.0
28	0.0021956596200630595	0.0	0.06894371206998007	0.016028315226460334	0.0
29	0.0021956596200630595	0.0	0.06894371206998007	0.03622838373104048	0.0
30	0.0021956596200630595	0.0	0.06894371206998007	0.06257629917179719	0.0
31	0.0021956596200630595	0.0	0.06894371206998007	0.13920481991199796	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCCG	25	0.0054929033	29.599998	25
TTAACGG	75	1.3691533E-8	24.666666	35
GTATCAA	1375	0.0	24.352728	1
GGTATCA	480	0.0	24.281248	1
AGACGTA	40	0.0019294067	23.125	24
TATTAGA	110	7.4578566E-11	21.863636	2
GCACCGC	95	7.1158865E-9	21.421053	10
CACCGCC	95	7.1158865E-9	21.421053	11
ATTAGAG	90	9.4467396E-8	20.555557	3
GTATACA	45	0.003822314	20.555557	1
TAACGGC	100	1.2845703E-8	20.349998	36
CTACCGA	55	5.136541E-4	20.181818	25
CGAGCCT	55	5.136541E-4	20.181818	29
TACCGAG	55	5.136541E-4	20.181818	26
ATATACC	65	6.890442E-5	19.923077	4
ATGCCCG	75	9.245641E-6	19.733334	29
AACGGCC	95	1.6705599E-7	19.473684	37
TTCGCCG	135	5.4569682E-11	19.185186	24
CGGTTGG	60	9.225149E-4	18.5	33
GCCGGCA	140	9.276846E-11	18.5	15
>>END_MODULE