FastQCFastQC Report
Fri 10 Feb 2017
ERR1633089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391240
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24760.6328596258051324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.4838462324915653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9180.23463858501175747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.12933237910234127No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4860.12422042735916573No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4810.12294243942337184No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4760.12166445148757796No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4450.11374092628565587No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4210.10760658419384521No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4130.10556180349657499No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4010.10249463245066966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4300.024.9534871
ACTGGAC551.8985023E-523.5454543
TCCGCCG952.8012437E-1023.36842231
TGGACCG400.001928922823.1250025
CTTGACT400.001928922823.1250023
GTATCAA11450.023.1048051
CGAGTCG1005.311449E-1022.19999921
TTCGCCG957.1086106E-921.42105324
TATTAGA705.0877406E-621.1428572
TCTAATC450.003821364420.5555572
GCTTTCG450.003821364420.55555724
TGACGCC555.134763E-420.18181812
ATACGGT555.134763E-420.1818184
ATTAGAT656.8872636E-519.9230773
ATAATAC656.8872636E-519.9230773
GGACCGT759.240173E-619.7333346
CTTCGCC1052.248089E-819.38095323
AGCTTCG1052.248089E-819.38095321
TGTCCGG1153.0322553E-919.30434614
TCGCCGT1205.142283E-918.525
Produced by FastQC (version 0.11.5)