##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633089.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391240
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.750817912278908	33.0	33.0	33.0	27.0	33.0
2	31.24544525099683	33.0	33.0	33.0	27.0	33.0
3	31.697352008997036	33.0	33.0	33.0	27.0	33.0
4	31.829317043247112	33.0	33.0	33.0	27.0	33.0
5	31.935154892137817	33.0	33.0	33.0	33.0	33.0
6	34.80532665371639	37.0	37.0	37.0	27.0	37.0
7	34.994001124629385	37.0	37.0	37.0	33.0	37.0
8	35.17045291892445	37.0	37.0	37.0	33.0	37.0
9	35.24973162253348	37.0	37.0	37.0	33.0	37.0
10	35.27001073509866	37.0	37.0	37.0	33.0	37.0
11	35.31495757080053	37.0	37.0	37.0	33.0	37.0
12	35.25721040793375	37.0	37.0	37.0	33.0	37.0
13	35.28634342091811	37.0	37.0	37.0	33.0	37.0
14	35.266639402924035	37.0	37.0	37.0	33.0	37.0
15	35.280178407115834	37.0	37.0	37.0	33.0	37.0
16	35.2956369491872	37.0	37.0	37.0	33.0	37.0
17	35.24261834168286	37.0	37.0	37.0	33.0	37.0
18	35.25040128821184	37.0	37.0	37.0	33.0	37.0
19	35.25080513239955	37.0	37.0	37.0	33.0	37.0
20	35.26054084449443	37.0	37.0	37.0	33.0	37.0
21	35.25795164093651	37.0	37.0	37.0	33.0	37.0
22	35.25197576934874	37.0	37.0	37.0	33.0	37.0
23	35.241171659339535	37.0	37.0	37.0	33.0	37.0
24	35.23881249361006	37.0	37.0	37.0	33.0	37.0
25	35.23191902668439	37.0	37.0	37.0	33.0	37.0
26	35.1974900316941	37.0	37.0	37.0	33.0	37.0
27	35.14127645435027	37.0	37.0	37.0	33.0	37.0
28	35.116054084449445	37.0	37.0	37.0	33.0	37.0
29	35.15658419384521	37.0	37.0	37.0	33.0	37.0
30	35.09707085165116	37.0	37.0	37.0	33.0	37.0
31	35.09070135977916	37.0	37.0	37.0	33.0	37.0
32	35.11933851344443	37.0	37.0	37.0	33.0	37.0
33	35.0901313771598	37.0	37.0	37.0	33.0	37.0
34	35.02979756671097	37.0	37.0	37.0	27.0	37.0
35	35.01509303752172	37.0	37.0	37.0	27.0	37.0
36	34.98892240057254	37.0	37.0	37.0	27.0	37.0
37	35.00225437071874	37.0	37.0	37.0	27.0	37.0
38	34.84115376750844	37.0	37.0	37.0	27.0	37.0
39	34.92020754524077	37.0	37.0	37.0	27.0	37.0
40	34.918387690420204	37.0	37.0	37.0	27.0	37.0
41	34.947638278294654	37.0	37.0	37.0	27.0	37.0
42	34.83929557304979	37.0	37.0	37.0	27.0	37.0
43	34.60920406911359	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	15.0
16	22.0
17	25.0
18	23.0
19	16.0
20	35.0
21	73.0
22	210.0
23	477.0
24	1036.0
25	1944.0
26	3164.0
27	4875.0
28	6843.0
29	9225.0
30	12104.0
31	15619.0
32	19533.0
33	25981.0
34	36487.0
35	65989.0
36	187542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97679173908598	19.582864737756875	13.707443001738065	24.732900521419076
2	16.373070238216954	23.134137613740926	37.742562110213676	22.75023003782844
3	18.0270422247214	27.02791125651774	30.015847050403842	24.92919946835702
4	12.756619977507414	17.736938963296186	38.77696554544525	30.729475513751147
5	14.478325324608935	36.36642470095082	34.44612002862693	14.709129945813313
6	29.361261629690215	39.784020038850834	16.78304876801963	14.07166956343932
7	26.892188937736428	32.02024332890298	22.7083120335344	18.379255699826196
8	24.842807483897353	34.98466414477047	20.15923729679992	20.013291074532255
9	26.620744300173804	14.682036601574481	19.72599938656579	38.971219711685926
10	15.874399345670176	27.213730702382172	32.935793886105714	23.97607606584194
11	34.668745527042226	23.282895409467336	23.22973111133831	18.81862795215213
12	23.759584909518455	25.53701053062059	29.289183110111438	21.414221449749515
13	28.868469481648095	21.987015642572334	25.458797668950005	23.68571720682957
14	22.87623964829772	20.506338820161538	27.6252428177078	28.99217871383294
15	24.889581842347408	27.51968101421123	24.629639096206933	22.961098047234437
16	22.971321950720785	27.734127389837436	24.938145383907575	24.3564052755342
17	23.003271649115632	26.3832941417033	25.7105612923014	24.902872916879666
18	23.992945506594417	25.843472037623965	27.389070647173092	22.774511808608526
19	25.770371127696556	25.110162560065437	27.08388712810551	22.0355791841325
20	25.258409160617525	24.875779572640834	27.123504754115118	22.74230651262652
21	23.682394438196503	26.26648604437174	26.984204069113588	23.066915448318166
22	23.42168489929455	25.962580513239953	27.12963909620693	23.486095491258563
23	23.215928841631737	26.00935487169001	27.69195378795624	23.082762498722012
24	23.611593906553523	26.229935589408036	26.41038748594213	23.74808301809631
25	23.732235967692464	25.92270728964319	27.588692362744094	22.756364379920253
26	23.64942234945302	26.12079541969124	27.14574174419793	23.084040486657806
27	23.700797464471933	26.037981801451792	26.86918515489214	23.39203557918413
28	23.111900623658112	25.781873019118702	27.515080257642367	23.591146099580822
29	24.18515489213782	25.92603005827625	26.734485226459466	23.15432982312647
30	24.087772211430327	25.77957264083427	27.166956343932114	22.965698803803292
31	23.723801247316224	25.67528882527349	27.13807381658317	23.462836110827116
32	22.7926592372968	25.70672732849402	27.473929046109806	24.026684388099376
33	22.881862795215213	25.768070749412125	27.810039873223598	23.540026582149064
34	23.407882629587977	25.44167263061037	27.61553010939577	23.53491463040589
35	23.276505469788365	26.345721296390963	26.971679787342808	23.406093446477865
36	23.649166751865863	26.183161230957978	26.955832736938962	23.211839280237196
37	23.399703506798897	25.653563030364996	27.241590839382475	23.705142623453636
38	23.38845721296391	26.099325222369902	26.873274716286677	23.63894284837951
39	23.027553419895717	25.71771802474185	27.423320723852367	23.83140783151007
40	23.086596462529393	26.35211123606993	27.577701666496267	22.98359063490441
41	23.52008997035068	25.34710152336162	27.267661793272673	23.86514671301503
42	22.77630099171864	26.468663735814335	27.282230855740725	23.472804416726305
43	23.674215315407423	25.418924445353237	27.14139658521624	23.765463654023105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	16.5
2	29.0
3	140.5
4	252.0
5	252.0
6	343.0
7	434.0
8	426.5
9	419.0
10	497.5
11	576.0
12	576.0
13	970.0
14	1364.0
15	2321.5
16	3279.0
17	3556.0
18	3833.0
19	3833.0
20	3193.0
21	2553.0
22	2325.5
23	2098.0
24	2684.0
25	3270.0
26	3270.0
27	4167.0
28	5064.0
29	6321.0
30	7578.0
31	9424.5
32	11271.0
33	11271.0
34	14076.5
35	16882.0
36	18804.0
37	20726.0
38	23047.5
39	25369.0
40	25369.0
41	27065.0
42	28761.0
43	29988.0
44	31215.0
45	31417.0
46	31619.0
47	31619.0
48	31752.0
49	31885.0
50	32229.0
51	32573.0
52	31183.5
53	29794.0
54	29794.0
55	28221.5
56	26649.0
57	23541.5
58	20434.0
59	18666.0
60	16898.0
61	16898.0
62	14747.0
63	12596.0
64	10465.0
65	8334.0
66	7211.0
67	6088.0
68	6088.0
69	4928.0
70	3768.0
71	3070.5
72	2373.0
73	1936.0
74	1499.0
75	1499.0
76	1110.5
77	722.0
78	570.0
79	418.0
80	347.0
81	276.0
82	276.0
83	220.5
84	165.0
85	132.5
86	100.0
87	74.0
88	48.0
89	48.0
90	34.0
91	20.0
92	12.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	391240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.8726170323917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.30986847837202	49.37105347532529
2	7.9088295034677865	9.154093220573435
3	2.380450640243691	4.132887248020044
4	1.1212382802502652	2.595559743799243
5	0.6939580715399676	2.0080584855384815
6	0.44332994223635835	1.539399837962226
7	0.32774165873569966	1.3277087251100372
8	0.2261463468036495	1.047014473747364
9	0.18347747700996503	0.9556489584960443
>10	1.198551052006611	13.35916827517891
>50	0.13240462019914856	5.28358329500584
>100	0.07222070192674959	7.731229894919049
>500	8.916136040339456E-4	0.3673920900475447
>1k	8.916136040339456E-4	1.1272022762764908
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2476	0.6328596258051324	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1893	0.4838462324915653	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	918	0.23463858501175747	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	506	0.12933237910234127	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	486	0.12422042735916573	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	481	0.12294243942337184	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	476	0.12166445148757796	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	445	0.11374092628565587	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	421	0.10760658419384521	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	413	0.10556180349657499	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	401	0.10249463245066966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0012779879357938861	0.0	0.0
2	2.555975871587772E-4	0.0	0.0012779879357938861	0.0	0.0
3	2.555975871587772E-4	0.0	0.0012779879357938861	0.0	0.0
4	2.555975871587772E-4	0.0	0.0017891831101114405	2.555975871587772E-4	0.0
5	5.111951743175544E-4	0.0	0.0028115734587465494	2.555975871587772E-4	0.0
6	5.111951743175544E-4	0.0	0.006901134853286985	7.667927614763316E-4	0.0
7	5.111951743175544E-4	0.0	0.01661384316532052	7.667927614763316E-4	0.0
8	5.111951743175544E-4	0.0	0.018658623862590738	7.667927614763316E-4	0.0
9	5.111951743175544E-4	0.0	0.021725794908496063	0.0025559758715877723	0.0
10	0.0015335855229526632	0.0	0.025048563541560168	0.0025559758715877723	0.0
11	0.0015335855229526632	0.0	0.03348328391779982	0.0025559758715877723	0.0
12	0.0015335855229526632	0.0	0.03859523566097536	0.0025559758715877723	0.0
13	0.0015335855229526632	0.0	0.042173601881198244	0.0025559758715877723	0.0
14	0.0015335855229526632	0.0	0.045240772927103566	0.0025559758715877723	0.0
15	0.0015335855229526632	0.0	0.05214190778039055	0.0025559758715877723	0.0
16	0.0015335855229526632	0.0	0.05546467641345466	0.0025559758715877723	0.0
17	0.0015335855229526632	0.0	0.0572538595235661	0.0025559758715877723	0.0
18	0.0017891831101114405	0.0	0.05878744504651876	0.0030671710459053265	0.0
19	0.0017891831101114405	0.0	0.05955423780799509	0.003322768633064104	0.0
20	0.0017891831101114405	0.0	0.06083222574378898	0.003833963807381658	0.0
21	0.0017891831101114405	0.0	0.06466618955117064	0.003833963807381658	0.0
22	0.0017891831101114405	0.0	0.06619977507412331	0.003833963807381658	0.0
23	0.0017891831101114405	0.0	0.06645537266128208	0.004345158981699212	0.0
24	0.0017891831101114405	0.0	0.06671097024844086	0.0048563541560167674	0.0
25	0.0017891831101114405	0.0	0.06696656783559964	0.0048563541560167674	0.0
26	0.0017891831101114405	0.0	0.06696656783559964	0.005367549330334322	0.0
27	0.0017891831101114405	0.0	0.06696656783559964	0.006901134853286985	0.0
28	0.0017891831101114405	0.0	0.06696656783559964	0.016869440752479297	0.0
29	0.0017891831101114405	0.0	0.06696656783559964	0.04064001635824558	0.0
30	0.0017891831101114405	0.0	0.06696656783559964	0.07361210510172785	0.0
31	0.0017891831101114405	0.0	0.06722216542275841	0.16562723647888764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	430	0.0	24.953487	1
ACTGGAC	55	1.8985023E-5	23.545454	3
TCCGCCG	95	2.8012437E-10	23.368422	31
TGGACCG	40	0.0019289228	23.125002	5
CTTGACT	40	0.0019289228	23.125002	3
GTATCAA	1145	0.0	23.104805	1
CGAGTCG	100	5.311449E-10	22.199999	21
TTCGCCG	95	7.1086106E-9	21.421053	24
TATTAGA	70	5.0877406E-6	21.142857	2
TCTAATC	45	0.0038213644	20.555557	2
GCTTTCG	45	0.0038213644	20.555557	24
TGACGCC	55	5.134763E-4	20.181818	12
ATACGGT	55	5.134763E-4	20.181818	4
ATTAGAT	65	6.8872636E-5	19.923077	3
ATAATAC	65	6.8872636E-5	19.923077	3
GGACCGT	75	9.240173E-6	19.733334	6
CTTCGCC	105	2.248089E-8	19.380953	23
AGCTTCG	105	2.248089E-8	19.380953	21
TGTCCGG	115	3.0322553E-9	19.304346	14
TCGCCGT	120	5.142283E-9	18.5	25
>>END_MODULE