FastQCFastQC Report
Fri 10 Feb 2017
ERR1633088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471338
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16980.3602510300463786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.2575646351450551No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC11080.23507546601377358No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7200.15275662051436548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6790.14405797962396413No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6670.1415120359487247No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6000.12729718376197124No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5520.11711340906101354No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5430.11520395130458397No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5060.10735395830592909No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4880.10353504279306994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTCG507.3123374E-933.30000321
TTGTCGC401.5974165E-632.37537
TAGACTG250.00549309429.65
GTCTATC250.00549309429.61
CGCTCTC652.9813236E-928.46153829
GCCGCTC604.3113687E-827.75000227
GCTTCGC604.3113687E-827.75000222
CCGCTCT706.5720087E-926.4285728
TTTGTCG509.076235E-625.90000236
GGTATCA3950.025.7594931
CGAGTCG551.8995728E-523.54545421
TCGGTAG551.8995728E-523.54545428
GGTACCT400.001929506123.12500218
GTATCAA10900.022.5733951
TAGAACC502.6988256E-422.225
GGACCGT502.6988256E-422.26
AGTCGCC603.7202844E-521.58333423
GTCGCCG705.0913914E-621.14285724
GGCTCGG705.0913914E-621.14285725
TTCGCCG806.9422094E-720.812524