##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633088.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	471338
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.736944612995345	33.0	33.0	33.0	27.0	33.0
2	31.272399000292783	33.0	33.0	33.0	27.0	33.0
3	31.70596260008741	33.0	33.0	33.0	27.0	33.0
4	31.84306803185824	33.0	33.0	33.0	27.0	33.0
5	31.946157534508146	33.0	33.0	33.0	33.0	33.0
6	34.834488201672684	37.0	37.0	37.0	27.0	37.0
7	35.034228091093865	37.0	37.0	37.0	33.0	37.0
8	35.193396246430375	37.0	37.0	37.0	33.0	37.0
9	35.2785771569447	37.0	37.0	37.0	33.0	37.0
10	35.29838672035779	37.0	37.0	37.0	33.0	37.0
11	35.348077176039276	37.0	37.0	37.0	33.0	37.0
12	35.292893846878464	37.0	37.0	37.0	33.0	37.0
13	35.32766507262304	37.0	37.0	37.0	33.0	37.0
14	35.30317097284751	37.0	37.0	37.0	33.0	37.0
15	35.32515095324374	37.0	37.0	37.0	33.0	37.0
16	35.32538008817452	37.0	37.0	37.0	33.0	37.0
17	35.28759191917477	37.0	37.0	37.0	33.0	37.0
18	35.298238206976734	37.0	37.0	37.0	33.0	37.0
19	35.29850340944291	37.0	37.0	37.0	33.0	37.0
20	35.29237617166449	37.0	37.0	37.0	33.0	37.0
21	35.30435059341704	37.0	37.0	37.0	33.0	37.0
22	35.29939448972924	37.0	37.0	37.0	33.0	37.0
23	35.275261065307696	37.0	37.0	37.0	33.0	37.0
24	35.29608900619089	37.0	37.0	37.0	33.0	37.0
25	35.29346880582512	37.0	37.0	37.0	33.0	37.0
26	35.260142403116234	37.0	37.0	37.0	33.0	37.0
27	35.19486016404364	37.0	37.0	37.0	33.0	37.0
28	35.16418366437673	37.0	37.0	37.0	33.0	37.0
29	35.19199597740899	37.0	37.0	37.0	33.0	37.0
30	35.15219226966635	37.0	37.0	37.0	33.0	37.0
31	35.15042708205152	37.0	37.0	37.0	33.0	37.0
32	35.18131998693082	37.0	37.0	37.0	33.0	37.0
33	35.182289567147144	37.0	37.0	37.0	33.0	37.0
34	35.110661563464014	37.0	37.0	37.0	33.0	37.0
35	35.097798183044866	37.0	37.0	37.0	33.0	37.0
36	35.09179612083049	37.0	37.0	37.0	33.0	37.0
37	35.11454624918848	37.0	37.0	37.0	33.0	37.0
38	34.96061637296378	37.0	37.0	37.0	27.0	37.0
39	35.04639558024178	37.0	37.0	37.0	27.0	37.0
40	35.0638734835723	37.0	37.0	37.0	27.0	37.0
41	35.08859883989833	37.0	37.0	37.0	33.0	37.0
42	35.00131540423221	37.0	37.0	37.0	27.0	37.0
43	34.80081173170846	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	17.0
16	26.0
17	29.0
18	15.0
19	25.0
20	27.0
21	95.0
22	212.0
23	527.0
24	1170.0
25	2137.0
26	3508.0
27	5417.0
28	7923.0
29	10727.0
30	13975.0
31	18187.0
32	23214.0
33	30388.0
34	43674.0
35	80083.0
36	229960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.29456992646466	20.010905125408943	13.432398830563205	25.262126117563195
2	16.318013824474157	22.400909750539952	38.264896952929746	23.016179472056148
3	17.84176111410495	26.913807076874768	29.982517853430025	25.261913955590252
4	12.726535946603073	17.75413821928213	39.228748795980806	30.290577038133993
5	14.020087495597638	37.03266021411386	35.062099809478546	13.885152480809952
6	29.790086943976508	39.55950931178899	17.258103526556315	13.392300217678185
7	26.923354365656916	31.91531342688262	23.379825093669513	17.781507113790955
8	24.455061972512297	35.84561397553348	20.460688508034576	19.23863554391965
9	26.27986710174015	15.023825789560782	20.04930644251047	38.647000666188596
10	15.664767109802307	27.547110566090577	33.49931471682741	23.2888076072797
11	34.32653424930729	23.586046531363905	23.733923426500727	18.353495792828074
12	23.732862616636044	25.48616916098426	29.456568322520145	21.324399899859547
13	27.7616063207295	21.96937229758687	25.975414670576104	24.293606711107525
14	22.19850722835842	20.676881558456987	27.463730910726486	29.66088030245811
15	24.48243086702112	27.907998082055762	24.547988916658532	23.061582134264583
16	23.141142874115815	27.77709414475387	24.936457489105482	24.145305492024832
17	22.725093245187107	27.075050176306597	25.85702828967747	24.342828288828823
18	23.14390097976399	26.753624787307622	27.456093079700768	22.646381153227622
19	25.277613941587568	25.550029914838184	26.72413427306943	22.448221870504817
20	24.536107846174083	25.914524184343296	27.127666345594882	22.421701623887742
21	23.98851779402467	26.5692560328257	26.694643758831244	22.747582414318387
22	23.29644543830542	26.568195222961016	26.74301668866079	23.392342650072774
23	23.339938642757428	26.58007629344547	27.145487951321556	22.934497112475547
24	23.82048551145887	26.89874357679627	25.676266288735473	23.60450462300939
25	23.652453228893066	26.537219574912267	27.174341979640936	22.635985216553724
26	23.82090983540474	26.73240859001396	26.988700253321397	22.457981321259904
27	23.850400349642932	26.589835744200553	26.051580818860348	23.508183087296167
28	22.85917961208305	26.394010243180055	27.484522784074272	23.262287360662622
29	24.56581052238521	26.19712393229487	26.46253856043858	22.774526984881337
30	24.079959604360354	26.013603825704696	27.032193457773403	22.874243112161547
31	23.5837127496616	26.09634699514998	26.977879992701624	23.342060262486793
32	22.48832048338984	26.068553776695282	27.337494536829198	24.105631203085682
33	22.81377694987461	26.421379137688877	27.480067382642602	23.284776529793906
34	23.440291255956446	26.354335954240902	27.300578353538224	22.90479443626442
35	23.730953158879615	26.65836406145908	26.699735646181722	22.910947133479585
36	24.182858161234613	26.559708744043554	26.155328023626357	23.102105071095476
37	23.251042776096984	26.12074562203769	26.749381547848888	23.87883005401644
38	23.783569328167896	26.359215679618448	26.62760057538327	23.229614416830387
39	22.956774119633895	26.417772384148954	27.043225880366105	23.582227615851046
40	23.300476515791217	26.70970725890974	27.3786539595789	22.611162265720143
41	23.123109106416205	25.61092040107099	27.46330658678061	23.802663905732192
42	22.602887948775614	27.01034077456093	27.004824563264577	23.381946713398875
43	23.599200573685973	25.335109836253388	27.21401626858008	23.85167332148055
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	14.5
2	22.0
3	99.5
4	177.0
5	177.0
6	217.0
7	257.0
8	262.5
9	268.0
10	330.5
11	393.0
12	393.0
13	643.5
14	894.0
15	1561.5
16	2229.0
17	2504.5
18	2780.0
19	2780.0
20	2519.0
21	2258.0
22	2521.0
23	2784.0
24	3721.5
25	4659.0
26	4659.0
27	6215.5
28	7772.0
29	9668.5
30	11565.0
31	14378.0
32	17191.0
33	17191.0
34	20544.0
35	23897.0
36	26547.0
37	29197.0
38	31588.0
39	33979.0
40	33979.0
41	35694.0
42	37409.0
43	38304.0
44	39199.0
45	38166.0
46	37133.0
47	37133.0
48	35893.5
49	34654.0
50	34687.0
51	34720.0
52	33139.0
53	31558.0
54	31558.0
55	31092.5
56	30627.0
57	26668.5
58	22710.0
59	20325.0
60	17940.0
61	17940.0
62	16420.0
63	14900.0
64	11937.5
65	8975.0
66	7532.5
67	6090.0
68	6090.0
69	5113.0
70	4136.0
71	3588.5
72	3041.0
73	3176.5
74	3312.0
75	3312.0
76	3095.0
77	2878.0
78	1968.5
79	1059.0
80	668.5
81	278.0
82	278.0
83	229.0
84	180.0
85	155.5
86	131.0
87	91.5
88	52.0
89	52.0
90	37.5
91	23.0
92	13.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	471338.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.01493831025706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.3379644239097	50.36583592082895
2	6.294445526784617	7.177548468137969
3	1.6842371113433892	2.8808002500926673
4	0.8220876482322023	1.8748510619833327
5	0.470446332161244	1.3411234303230013
6	0.34861065773937616	1.1925609087185203
7	0.2360092607085063	0.9419237407961405
8	0.1768634915858788	0.806708884968444
9	0.15096920337928035	0.774674983567649
>10	1.20826145267796	13.830217422367674
>50	0.165147698277867	6.59412286860104
>100	0.10119528663366936	10.442195935237237
>500	0.002633334596415188	0.9152069437333324
>1k	0.0011285719698922235	0.8622291806440116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1698	0.3602510300463786	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1214	0.2575646351450551	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1108	0.23507546601377358	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	720	0.15275662051436548	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.14405797962396413	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	667	0.1415120359487247	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	600	0.12729718376197124	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	552	0.11711340906101354	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	543	0.11520395130458397	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	506	0.10735395830592909	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	488	0.10353504279306994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.1216197293661874E-4	0.0	0.0
2	2.1216197293661874E-4	0.0	2.1216197293661874E-4	0.0	0.0
3	2.1216197293661874E-4	0.0	4.243239458732375E-4	0.0	0.0
4	2.1216197293661874E-4	0.0	8.48647891746475E-4	0.0	0.0
5	2.1216197293661874E-4	0.0	8.48647891746475E-4	0.0	0.0
6	2.1216197293661874E-4	0.0	0.002121619729366187	0.0	0.0
7	2.1216197293661874E-4	0.0	0.006152697215161943	0.0	0.0
8	2.1216197293661874E-4	0.0	0.007213507079845037	0.0	0.0
9	2.1216197293661874E-4	0.0	0.008698640890401368	4.243239458732375E-4	0.0
10	6.364859188098562E-4	0.0	0.010395936673894319	4.243239458732375E-4	0.0
11	6.364859188098562E-4	0.0	0.015275662051436548	4.243239458732375E-4	0.0
12	6.364859188098562E-4	0.0	0.01676079586199288	4.243239458732375E-4	0.0
13	6.364859188098562E-4	0.0	0.017821605726675974	4.243239458732375E-4	0.0
14	6.364859188098562E-4	0.0	0.018882415591359065	6.364859188098562E-4	0.0
15	8.48647891746475E-4	0.0	0.02142835926659849	8.48647891746475E-4	0.0
16	8.48647891746475E-4	0.0	0.023974302941837916	8.48647891746475E-4	0.0
17	8.48647891746475E-4	0.0	0.02545943675239425	8.48647891746475E-4	0.0
18	8.48647891746475E-4	0.0	0.026308084644140722	8.48647891746475E-4	0.0
19	8.48647891746475E-4	0.0	0.027581056481760437	8.48647891746475E-4	0.0
20	8.48647891746475E-4	0.0	0.02821754240057029	0.0010608098646830936	0.0
21	8.48647891746475E-4	0.0	0.02864186634644353	0.001485133810556331	0.0
22	8.48647891746475E-4	0.0	0.029066190292316767	0.00169729578349295	0.0
23	8.48647891746475E-4	0.0	0.029278352265253386	0.00169729578349295	0.0
24	8.48647891746475E-4	0.0	0.029490514238190005	0.00169729578349295	0.0
25	8.48647891746475E-4	0.0	0.029490514238190005	0.00169729578349295	0.0
26	8.48647891746475E-4	0.0	0.029490514238190005	0.002121619729366187	0.0
27	8.48647891746475E-4	0.0	0.029490514238190005	0.0027581056481760435	0.0
28	8.48647891746475E-4	0.0	0.029490514238190005	0.010820260619767555	0.0
29	8.48647891746475E-4	0.0	0.029490514238190005	0.027581056481760437	0.0
30	8.48647891746475E-4	0.0	0.029490514238190005	0.0543134650717744	0.0
31	8.48647891746475E-4	0.0	0.029490514238190005	0.13196474716657686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTTCG	50	7.3123374E-9	33.300003	21
TTGTCGC	40	1.5974165E-6	32.375	37
TAGACTG	25	0.005493094	29.6	5
GTCTATC	25	0.005493094	29.6	1
CGCTCTC	65	2.9813236E-9	28.461538	29
GCCGCTC	60	4.3113687E-8	27.750002	27
GCTTCGC	60	4.3113687E-8	27.750002	22
CCGCTCT	70	6.5720087E-9	26.42857	28
TTTGTCG	50	9.076235E-6	25.900002	36
GGTATCA	395	0.0	25.759493	1
CGAGTCG	55	1.8995728E-5	23.545454	21
TCGGTAG	55	1.8995728E-5	23.545454	28
GGTACCT	40	0.0019295061	23.125002	18
GTATCAA	1090	0.0	22.573395	1
TAGAACC	50	2.6988256E-4	22.2	25
GGACCGT	50	2.6988256E-4	22.2	6
AGTCGCC	60	3.7202844E-5	21.583334	23
GTCGCCG	70	5.0913914E-6	21.142857	24
GGCTCGG	70	5.0913914E-6	21.142857	25
TTCGCCG	80	6.9422094E-7	20.8125	24
>>END_MODULE