FastQCFastQC Report
Fri 10 Feb 2017
ERR1633087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495635
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14410.29073814399709463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.2090247863851423No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7470.15071574848426764No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7470.15071574848426764No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7310.14748756645515348No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6690.1349783610923361No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6340.12791671290364887No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5520.11137228000443875No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5360.1081440979753246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5320.10733705246804605No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5010.10108244978663733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTG251.2326127E-436.99999611
GCGAATA250.005493364829.5999987
GTCTAAT358.862248E-426.4285721
GGTATCA3500.024.8428571
CGAGTCG451.3217289E-424.66666621
GTATCAA9900.023.9191931
CGCTCTC551.8998291E-523.54545429
CAAACGC400.001929645823.12530
TAGCGGT400.001929645823.12518
CTAGTCC400.001929645823.1259
TATTGTC1155.456968E-1222.521745
GTCGTGT603.7207825E-521.58333412
CGTGTAG603.7207825E-521.58333414
TGTCCGG603.7207825E-521.58333414
TCTATAC555.1374227E-420.1818183
CGTCCGC656.892008E-519.92307729
TTCGCCG656.892008E-519.92307724
ATTGTCT1403.6379788E-1219.821436
GTCGCCA1153.0377123E-919.30434812
CCGATCT701.2174222E-418.59
Produced by FastQC (version 0.11.5)