##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633087.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495635
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75478729306849	33.0	33.0	33.0	27.0	33.0
2	31.279241780745913	33.0	33.0	33.0	27.0	33.0
3	31.70653000696077	33.0	33.0	33.0	27.0	33.0
4	31.845870449019944	33.0	33.0	33.0	27.0	33.0
5	31.962264569693424	33.0	33.0	33.0	33.0	33.0
6	34.83406337324846	37.0	37.0	37.0	27.0	37.0
7	35.0160945050289	37.0	37.0	37.0	33.0	37.0
8	35.18300967445802	37.0	37.0	37.0	33.0	37.0
9	35.26544332018522	37.0	37.0	37.0	33.0	37.0
10	35.307318893944135	37.0	37.0	37.0	33.0	37.0
11	35.32849375044135	37.0	37.0	37.0	33.0	37.0
12	35.2834928929555	37.0	37.0	37.0	33.0	37.0
13	35.32156122953383	37.0	37.0	37.0	33.0	37.0
14	35.29304427653414	37.0	37.0	37.0	33.0	37.0
15	35.30362060790703	37.0	37.0	37.0	33.0	37.0
16	35.3122882766552	37.0	37.0	37.0	33.0	37.0
17	35.27013830742381	37.0	37.0	37.0	33.0	37.0
18	35.28064200470104	37.0	37.0	37.0	33.0	37.0
19	35.28175370988732	37.0	37.0	37.0	33.0	37.0
20	35.29598192218064	37.0	37.0	37.0	33.0	37.0
21	35.27881202901329	37.0	37.0	37.0	33.0	37.0
22	35.28214916218588	37.0	37.0	37.0	33.0	37.0
23	35.27573315040302	37.0	37.0	37.0	33.0	37.0
24	35.27385273437106	37.0	37.0	37.0	33.0	37.0
25	35.27491198159936	37.0	37.0	37.0	33.0	37.0
26	35.22376749018935	37.0	37.0	37.0	33.0	37.0
27	35.18293098752106	37.0	37.0	37.0	33.0	37.0
28	35.15246703723506	37.0	37.0	37.0	33.0	37.0
29	35.20726744479305	37.0	37.0	37.0	33.0	37.0
30	35.16987702644083	37.0	37.0	37.0	33.0	37.0
31	35.15221281790027	37.0	37.0	37.0	33.0	37.0
32	35.19468762294834	37.0	37.0	37.0	33.0	37.0
33	35.16247238391155	37.0	37.0	37.0	33.0	37.0
34	35.1184863861511	37.0	37.0	37.0	33.0	37.0
35	35.129924238603	37.0	37.0	37.0	33.0	37.0
36	35.110379614030485	37.0	37.0	37.0	33.0	37.0
37	35.132978905848056	37.0	37.0	37.0	33.0	37.0
38	34.975851180808455	37.0	37.0	37.0	27.0	37.0
39	35.050880184006374	37.0	37.0	37.0	27.0	37.0
40	35.10650377798178	37.0	37.0	37.0	33.0	37.0
41	35.11633964510174	37.0	37.0	37.0	33.0	37.0
42	35.034573829531816	37.0	37.0	37.0	27.0	37.0
43	34.82056755475299	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	14.0
16	33.0
17	38.0
18	25.0
19	17.0
20	34.0
21	79.0
22	262.0
23	606.0
24	1210.0
25	2324.0
26	3872.0
27	5831.0
28	8333.0
29	11094.0
30	14690.0
31	19020.0
32	24190.0
33	32014.0
34	45370.0
35	83509.0
36	243068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.892188808296424	20.541124012630263	13.915885682003895	24.650801497069416
2	15.715798924611862	23.28628930563822	38.83704742401162	22.160864345738297
3	17.69265689469065	27.002935628032727	31.253644314868804	24.050763162407822
4	12.731344638695816	18.236000282465927	39.1697519343872	29.86290314445106
5	13.484318096986694	37.538712964177265	35.75716000685989	13.219808931976152
6	28.961231551444104	39.8418190805734	17.757220535272932	13.439728832709555
7	25.70016241790834	32.763626458986955	23.910337244141356	17.62587387896335
8	23.72976081188778	36.632804382257106	20.939602731849043	18.697832074006072
9	25.64407275515248	16.062424969987994	20.256842232691397	38.03666004216813
10	14.73160692848568	28.50061032816488	34.25282718129268	22.514955562056755
11	32.904455900007065	24.256761528140668	24.490602963874625	18.348179607977645
12	22.44151442089441	26.20880284887064	30.15606242496999	21.19362030526496
13	27.196626549779573	22.64428460459814	27.063665802455432	23.095423043166846
14	21.88182836159674	21.539842827887455	27.7805239742956	28.7978048362202
15	23.798964964136914	28.82625319035177	25.072281013245636	22.30250083226568
16	22.73507722416698	29.071393263187627	25.067842262955605	23.12568724968979
17	22.39571458835635	27.935678473069903	26.210618701262018	23.45798823731173
18	22.28514935385919	27.8021124416153	27.81764806763041	22.09509013689509
19	24.723839115478125	26.665590605990296	27.79222613415114	20.81834414438044
20	24.379432445247005	26.15372199299888	27.98026773734704	21.486577824407075
21	22.49477942437479	27.91328296024292	26.95451289759601	22.637424717786274
22	22.1465392879841	27.649177317986016	27.015243072018723	23.189040322011156
23	22.26416617066995	27.563630494214493	27.876360628284928	22.295842706830634
24	23.336326127089492	27.79464727067297	26.466250365692495	22.402776236545037
25	23.138196455052608	27.106237452964375	27.536796231097483	22.21876986088553
26	22.941479112653465	27.329789058480536	27.125203022385424	22.603528806480575
27	22.830913878156306	27.257356724202285	26.855044538823936	23.056684858817476
28	21.861046939784316	27.547287822692102	27.95363523560685	22.638030001916736
29	23.483813693544644	27.54184026551797	26.838096583171083	22.1362494577663
30	22.609581647785166	27.627790612043135	27.320911557900473	22.44171618227123
31	23.072220484832588	27.244040473332188	27.406054858918356	22.277684182916865
32	21.773482502244594	27.20631109586692	27.65280902276877	23.367397379119716
33	22.131205423345808	27.03259455042521	28.00609319357995	22.830106832649026
34	22.204243041754516	27.042480857889373	27.696793002915456	23.05648309744066
35	22.730840235253766	27.612658508781664	27.224469619780685	22.432031636183886
36	23.034289345990498	27.44438952051409	26.97307494426342	22.548246189231996
37	23.31090419361022	26.61172031837945	27.063464041078618	23.013911446931715
38	23.377687209337516	27.00979551484459	27.173827514198955	22.43868976161893
39	22.708242961049965	27.004549719047283	27.51500600240096	22.772201317501793
40	22.56741352002986	27.201872345576888	27.62193953211537	22.608774602277887
41	22.434452772705722	26.757392032443228	27.915502335387938	22.892652859463112
42	22.76271853279127	27.01766420854056	27.323130933045487	22.896486325622686
43	22.577299827494024	26.399063827211556	27.631220555449072	23.39241578984535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	18.5
2	28.0
3	93.0
4	158.0
5	158.0
6	200.5
7	243.0
8	232.0
9	221.0
10	306.5
11	392.0
12	392.0
13	588.0
14	784.0
15	1486.5
16	2189.0
17	2535.5
18	2882.0
19	2882.0
20	2873.5
21	2865.0
22	3480.5
23	4096.0
24	5282.5
25	6469.0
26	6469.0
27	8259.5
28	10050.0
29	13730.0
30	17410.0
31	19388.5
32	21367.0
33	21367.0
34	23815.5
35	26264.0
36	28643.5
37	31023.0
38	34189.0
39	37355.0
40	37355.0
41	39841.5
42	42328.0
43	40610.5
44	38893.0
45	38752.0
46	38611.0
47	38611.0
48	37969.0
49	37327.0
50	37764.5
51	38202.0
52	38282.0
53	38362.0
54	38362.0
55	33440.5
56	28519.0
57	24936.0
58	21353.0
59	18346.5
60	15340.0
61	15340.0
62	13440.5
63	11541.0
64	9381.0
65	7221.0
66	6175.5
67	5130.0
68	5130.0
69	4236.5
70	3343.0
71	2824.0
72	2305.0
73	1906.5
74	1508.0
75	1508.0
76	1132.0
77	756.0
78	606.5
79	457.0
80	335.0
81	213.0
82	213.0
83	182.5
84	152.0
85	145.0
86	138.0
87	107.5
88	77.0
89	77.0
90	61.5
91	46.0
92	26.5
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	495635.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.679855271342134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.659865035133	48.479085884978
2	5.951222331248983	6.508239515205476
3	1.6128667602512599	2.6457396306749206
4	0.7981068413399856	1.7456146630215372
5	0.481524458340891	1.3164843845845668
6	0.33696740457989244	1.1055197348152987
7	0.23561922079616532	0.9018537424596509
8	0.18652387804727882	0.8159278925019741
9	0.15719610813257592	0.7735914397716751
>10	1.2581960893291195	14.254550894781296
>50	0.1871926844249754	7.260813155683765
>100	0.1306255113809732	12.538999629965097
>500	0.0033493720866916202	1.1495286990972402
>1k	7.443049081536934E-4	0.5040507324595263
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1441	0.29073814399709463	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1036	0.2090247863851423	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	747	0.15071574848426764	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	747	0.15071574848426764	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	731	0.14748756645515348	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	669	0.1349783610923361	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	634	0.12791671290364887	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	552	0.11137228000443875	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	536	0.1081440979753246	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	532	0.10733705246804605	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	501	0.10108244978663733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.0352275363927085E-4	2.0176137681963543E-4	0.0
2	0.0	0.0	4.0352275363927085E-4	2.0176137681963543E-4	0.0
3	0.0	0.0	4.0352275363927085E-4	2.0176137681963543E-4	0.0
4	0.0	0.0	6.052841304589063E-4	2.0176137681963543E-4	0.0
5	0.0	0.0	6.052841304589063E-4	2.0176137681963543E-4	0.0
6	0.0	0.0	8.070455072785417E-4	2.0176137681963543E-4	0.0
7	0.0	0.0	0.0036317047827534375	2.0176137681963543E-4	0.0
8	0.0	0.0	0.004236988913212344	2.0176137681963543E-4	0.0
9	0.0	0.0	0.00484227304367125	0.0010088068840981772	0.0
10	4.0352275363927085E-4	0.0	0.005447557174130156	0.0010088068840981772	0.0
11	6.052841304589063E-4	0.0	0.008877500580063959	0.0010088068840981772	0.0
12	6.052841304589063E-4	0.0	0.00928102333370323	0.0010088068840981772	0.0
13	6.052841304589063E-4	0.0	0.009482784710522864	0.0014123296377374478	0.0
14	6.052841304589063E-4	0.0	0.009886307464162136	0.0014123296377374478	0.0
15	8.070455072785417E-4	0.0	0.010895114348260312	0.0014123296377374478	0.0
16	8.070455072785417E-4	0.0	0.011702159855538853	0.0014123296377374478	0.0
17	8.070455072785417E-4	0.0	0.01230744398599776	0.0016140910145570834	0.0
18	8.070455072785417E-4	0.0	0.012912728116456667	0.0016140910145570834	0.0
19	8.070455072785417E-4	0.0	0.013114489493276302	0.0016140910145570834	0.0
20	8.070455072785417E-4	0.0	0.013518012246915572	0.0018158523913767188	0.0
21	8.070455072785417E-4	0.0	0.013921535000554843	0.0020176137681963543	0.0
22	8.070455072785417E-4	0.0	0.013921535000554843	0.0022193751450159897	0.0
23	8.070455072785417E-4	0.0	0.013921535000554843	0.002421136521835625	0.0
24	8.070455072785417E-4	0.0	0.01412329637737448	0.003026420652294531	0.0
25	8.070455072785417E-4	0.0	0.01412329637737448	0.0036317047827534375	0.0
26	8.070455072785417E-4	0.0	0.01412329637737448	0.0036317047827534375	0.0
27	8.070455072785417E-4	0.0	0.01412329637737448	0.004035227536392709	0.0
28	8.070455072785417E-4	0.0	0.01412329637737448	0.010895114348260312	0.0
29	8.070455072785417E-4	2.0176137681963543E-4	0.01412329637737448	0.021790228696520624	0.0
30	8.070455072785417E-4	2.0176137681963543E-4	0.01412329637737448	0.042168127755303805	0.0
31	8.070455072785417E-4	2.0176137681963543E-4	0.01412329637737448	0.11036347312034057	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGTG	25	1.2326127E-4	36.999996	11
GCGAATA	25	0.0054933648	29.599998	7
GTCTAAT	35	8.862248E-4	26.428572	1
GGTATCA	350	0.0	24.842857	1
CGAGTCG	45	1.3217289E-4	24.666666	21
GTATCAA	990	0.0	23.919193	1
CGCTCTC	55	1.8998291E-5	23.545454	29
CAAACGC	40	0.0019296458	23.125	30
TAGCGGT	40	0.0019296458	23.125	18
CTAGTCC	40	0.0019296458	23.125	9
TATTGTC	115	5.456968E-12	22.52174	5
GTCGTGT	60	3.7207825E-5	21.583334	12
CGTGTAG	60	3.7207825E-5	21.583334	14
TGTCCGG	60	3.7207825E-5	21.583334	14
TCTATAC	55	5.1374227E-4	20.181818	3
CGTCCGC	65	6.892008E-5	19.923077	29
TTCGCCG	65	6.892008E-5	19.923077	24
ATTGTCT	140	3.6379788E-12	19.82143	6
GTCGCCA	115	3.0377123E-9	19.304348	12
CCGATCT	70	1.2174222E-4	18.5	9
>>END_MODULE