FastQCFastQC Report
Fri 10 Feb 2017
ERR1633086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396973
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10270.25870777105747744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.1937159454169427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATT250.00549206429.59999832
GTATCAA6000.026.8251
GGTATCA1650.024.6666681
GTGCCGG400.001928972723.12511
GTAATAC502.6977865E-422.1999993
GTCGCCC806.936516E-720.812537
TGCGAAT450.003821463320.55555533
TATAAGG450.003821463320.5555552
TCCGGGA450.003821463320.55555537
GAGTGAC500.007026970418.4999987
ATATTAG500.007026970418.4999981
TCAACGC8900.018.0842694
TGCTCGG1552.0008883E-1117.90322510
ATCAACG8950.017.7765373
CAACGCA9050.017.580115
GGTCGCC953.5943667E-617.52631636
AGGTCGC953.5943667E-617.52631635
CAACACT852.7158509E-517.4117644
CTGCTCG1603.274181E-1117.343759
TCTCGTC752.0629737E-417.26666612
Produced by FastQC (version 0.11.5)