##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	396973
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.708201817252053	33.0	33.0	33.0	27.0	33.0
2	31.233522179090265	33.0	33.0	33.0	27.0	33.0
3	31.671063271305606	33.0	33.0	33.0	27.0	33.0
4	31.80151546830641	33.0	33.0	33.0	27.0	33.0
5	31.909112206623625	33.0	33.0	33.0	33.0	33.0
6	34.78594010171977	37.0	37.0	37.0	27.0	37.0
7	34.969496666020106	37.0	37.0	37.0	27.0	37.0
8	35.131719285694494	37.0	37.0	37.0	33.0	37.0
9	35.20980268179448	37.0	37.0	37.0	33.0	37.0
10	35.2651993964325	37.0	37.0	37.0	33.0	37.0
11	35.27823806656876	37.0	37.0	37.0	33.0	37.0
12	35.237552679905185	37.0	37.0	37.0	33.0	37.0
13	35.27104110355112	37.0	37.0	37.0	33.0	37.0
14	35.239965942268114	37.0	37.0	37.0	33.0	37.0
15	35.246651535494856	37.0	37.0	37.0	33.0	37.0
16	35.2471226002776	37.0	37.0	37.0	33.0	37.0
17	35.20648255674819	37.0	37.0	37.0	33.0	37.0
18	35.223264554516305	37.0	37.0	37.0	33.0	37.0
19	35.23339874500281	37.0	37.0	37.0	33.0	37.0
20	35.235202394117486	37.0	37.0	37.0	33.0	37.0
21	35.21989656727284	37.0	37.0	37.0	33.0	37.0
22	35.216264078413396	37.0	37.0	37.0	33.0	37.0
23	35.22164731606431	37.0	37.0	37.0	33.0	37.0
24	35.219128253055	37.0	37.0	37.0	33.0	37.0
25	35.21086824544742	37.0	37.0	37.0	33.0	37.0
26	35.17277245555743	37.0	37.0	37.0	33.0	37.0
27	35.093782196774086	37.0	37.0	37.0	33.0	37.0
28	35.09078954991901	37.0	37.0	37.0	33.0	37.0
29	35.11046847014784	37.0	37.0	37.0	33.0	37.0
30	35.09938711196983	37.0	37.0	37.0	33.0	37.0
31	35.07532502210478	37.0	37.0	37.0	33.0	37.0
32	35.106319573371486	37.0	37.0	37.0	33.0	37.0
33	35.08919750209712	37.0	37.0	37.0	33.0	37.0
34	35.02479261813776	37.0	37.0	37.0	27.0	37.0
35	35.032473241253186	37.0	37.0	37.0	27.0	37.0
36	35.02074196481877	37.0	37.0	37.0	27.0	37.0
37	35.03291911540583	37.0	37.0	37.0	27.0	37.0
38	34.88681597992811	37.0	37.0	37.0	27.0	37.0
39	34.97282182919241	37.0	37.0	37.0	27.0	37.0
40	35.01047930211878	37.0	37.0	37.0	27.0	37.0
41	35.022835306179516	37.0	37.0	37.0	27.0	37.0
42	34.943575507654174	37.0	37.0	37.0	27.0	37.0
43	34.71965599675545	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	12.0
16	22.0
17	25.0
18	21.0
19	15.0
20	26.0
21	83.0
22	238.0
23	561.0
24	1144.0
25	2003.0
26	3374.0
27	4994.0
28	6969.0
29	9407.0
30	12341.0
31	15549.0
32	19957.0
33	25853.0
34	37067.0
35	66800.0
36	190510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15292475810698	19.980703977348586	13.528124078967588	25.33824718557685
2	16.003355391928416	22.794497358762435	38.080675511936576	23.121471737372566
3	18.085612875434855	26.271811936831973	30.46227325284087	25.180301934892295
4	13.18099719628287	17.670219385197484	38.289002022807594	30.859781395712048
5	13.85635798908238	37.04937111591972	35.41953734888771	13.674733546110188
6	30.02899441523731	39.122056159990734	17.459373811317143	13.389575613454818
7	26.65924382766586	32.083038393039324	23.460033805825585	17.797683973469226
8	24.43440737783174	36.24503429704287	20.619034543911045	18.70152378121434
9	25.803266217097885	15.814677572530123	19.935864655782634	38.446191554589355
10	14.813098120023277	28.296382877424914	33.988457653291285	22.90206134926053
11	33.88190128799692	23.497819750965434	23.977701254241474	18.642577706796178
12	22.76552813415522	25.438757799648844	30.28190834137334	21.513805724822596
13	27.86209641461762	22.071274368785787	26.51490151723165	23.551727699364942
14	22.284639005675448	20.816277177541043	27.42428326359727	29.47480055318624
15	23.847213790358538	28.570205026538332	24.314248072287032	23.2683331108161
16	23.32274487181748	28.49261788585118	24.805213452804097	23.379423789527248
17	22.65494126804594	27.633113587070152	26.344360951500477	23.36758419338343
18	22.901557536658665	27.360299063160465	27.510435218516122	22.22770818166475
19	24.785564761331376	26.206568204890505	27.20915528260108	21.798711751177034
20	24.544994243941023	26.052905361321805	27.42780995181032	21.97429044292685
21	23.074365259098226	26.898051000949685	26.808120451516853	23.219463288435232
22	22.642849765601188	26.870845120449	26.924501162547575	23.561803951402236
23	22.695246276195107	27.02299652621211	27.423779450995404	22.857977746597374
24	23.310401463071795	27.11695757645986	26.48920707453655	23.08343388593179
25	23.422247860685736	26.736075249450213	27.132323860816733	22.709353029047318
26	23.373881850906734	26.940875072108177	26.919966849130798	22.765276227854287
27	23.34037831288274	26.732548561237163	26.3725744572049	23.5544986686752
28	22.807848392711847	26.858249805402384	27.34619231030826	22.98770949157751
29	23.532330914193157	26.764540661455566	26.84086827063805	22.862260153713223
30	23.147669992669524	27.027782745929823	27.07010300448645	22.754444256914198
31	23.368339912286228	26.81744098465135	26.736830968353008	23.077388134709416
32	22.342577454889877	26.77713597650218	27.338131308678427	23.54215525992952
33	22.740337504061987	26.598030596539314	27.669640000705336	22.99199189869336
34	23.127769394895875	26.457466880619084	27.169857899655643	23.244905824829395
35	23.27488267464034	27.130812423011136	26.944653666622166	22.64965123572636
36	23.412423514949378	26.689220677476804	26.985714393674133	22.91264141389969
37	23.62226146362599	26.311109319777415	26.810639514526173	23.25598970207042
38	23.553994856073334	26.620954069924153	26.74237290697352	23.08267816702899
39	23.167318684142245	26.647404231522042	27.136102455330715	23.049174629004995
40	22.964786018192672	26.923241631042917	27.294299612316202	22.81767273844821
41	22.72068881258927	26.205056767084915	27.62933499255617	23.444919427769648
42	22.98619805377192	26.598030596539314	27.03408040345313	23.38169094623564
43	23.2441501059266	26.078347897715965	27.27187995153323	23.405622044824206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	18.5
2	24.0
3	67.5
4	111.0
5	111.0
6	133.0
7	155.0
8	150.5
9	146.0
10	201.0
11	256.0
12	256.0
13	395.0
14	534.0
15	1050.5
16	1567.0
17	1799.0
18	2031.0
19	2031.0
20	2140.0
21	2249.0
22	2724.0
23	3199.0
24	4267.5
25	5336.0
26	5336.0
27	6784.5
28	8233.0
29	10460.5
30	12688.0
31	14617.0
32	16546.0
33	16546.0
34	18473.0
35	20400.0
36	22308.5
37	24217.0
38	26353.0
39	28489.0
40	28489.0
41	29204.5
42	29920.0
43	29640.0
44	29360.0
45	29619.5
46	29879.0
47	29879.0
48	29087.0
49	28295.0
50	28899.5
51	29504.0
52	29449.5
53	29395.0
54	29395.0
55	26736.0
56	24077.0
57	21682.0
58	19287.0
59	17254.0
60	15221.0
61	15221.0
62	13749.5
63	12278.0
64	10162.5
65	8047.0
66	6800.5
67	5554.0
68	5554.0
69	4585.0
70	3616.0
71	3116.5
72	2617.0
73	2125.5
74	1634.0
75	1634.0
76	1255.5
77	877.0
78	695.5
79	514.0
80	396.5
81	279.0
82	279.0
83	218.5
84	158.0
85	159.0
86	160.0
87	116.5
88	73.0
89	73.0
90	51.0
91	29.0
92	17.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	396973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.73887665325114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.63095278813672	58.26499272999446
2	6.371226531740187	8.376745501999785
3	1.6935183438161305	3.3398998054244027
4	0.8288969239352	2.179630105633416
5	0.4808124942381334	1.580403662603134
6	0.31304595676918684	1.2347573723309135
7	0.23534438197842472	1.0829892708540698
8	0.16869612010351823	0.887191474509378
9	0.131552615440492	0.7783309043869575
>10	1.0102963086312662	13.434577564571562
>50	0.09237080033047537	4.036208961729901
>100	0.042513757471730244	4.347956940628707
>500	3.8648870428845675E-4	0.19538239276243066
>1k	3.8648870428845675E-4	0.2609333125708924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1027	0.25870777105747744	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	769	0.1937159454169427	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	5.038126018646104E-4	0.0	0.0
7	0.0	0.0	7.557189027969156E-4	0.0	0.0
8	0.0	0.0	0.0010076252037292208	0.0	0.0
9	0.0	0.0	0.0017633441065261365	0.0	0.0
10	0.0	0.0	0.0017633441065261365	0.0	0.0
11	0.0	0.0	0.002267156708390747	0.0	0.0
12	0.0	0.0	0.0027709693102553573	0.0	0.0
13	0.0	0.0	0.0030228756111876625	0.0	0.0
14	0.0	0.0	0.0030228756111876625	0.0	0.0
15	0.0	0.0	0.003778594513984578	0.0	0.0
16	0.0	0.0	0.004282407115849189	2.519063009323052E-4	0.0
17	0.0	0.0	0.004282407115849189	2.519063009323052E-4	0.0
18	0.0	0.0	0.004534313416781494	2.519063009323052E-4	0.0
19	0.0	0.0	0.004534313416781494	2.519063009323052E-4	0.0
20	0.0	0.0	0.004786219717713799	2.519063009323052E-4	0.0
21	0.0	0.0	0.004786219717713799	2.519063009323052E-4	0.0
22	0.0	0.0	0.004786219717713799	2.519063009323052E-4	0.0
23	0.0	0.0	0.004786219717713799	2.519063009323052E-4	0.0
24	0.0	0.0	0.004786219717713799	2.519063009323052E-4	0.0
25	0.0	0.0	0.004786219717713799	5.038126018646104E-4	0.0
26	0.0	0.0	0.004786219717713799	7.557189027969156E-4	0.0
27	0.0	0.0	0.004786219717713799	0.0015114378055938313	0.0
28	0.0	0.0	0.004786219717713799	0.006297657523307631	0.0
29	0.0	0.0	0.004786219717713799	0.015618190657802924	0.0
30	0.0	0.0	0.004786219717713799	0.033251631723064286	0.0
31	0.0	0.0	0.004786219717713799	0.08061001629833767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTATT	25	0.005492064	29.599998	32
GTATCAA	600	0.0	26.825	1
GGTATCA	165	0.0	24.666668	1
GTGCCGG	40	0.0019289727	23.125	11
GTAATAC	50	2.6977865E-4	22.199999	3
GTCGCCC	80	6.936516E-7	20.8125	37
TGCGAAT	45	0.0038214633	20.555555	33
TATAAGG	45	0.0038214633	20.555555	2
TCCGGGA	45	0.0038214633	20.555555	37
GAGTGAC	50	0.0070269704	18.499998	7
ATATTAG	50	0.0070269704	18.499998	1
TCAACGC	890	0.0	18.084269	4
TGCTCGG	155	2.0008883E-11	17.903225	10
ATCAACG	895	0.0	17.776537	3
CAACGCA	905	0.0	17.58011	5
GGTCGCC	95	3.5943667E-6	17.526316	36
AGGTCGC	95	3.5943667E-6	17.526316	35
CAACACT	85	2.7158509E-5	17.411764	4
CTGCTCG	160	3.274181E-11	17.34375	9
TCTCGTC	75	2.0629737E-4	17.266666	12
>>END_MODULE