FastQCFastQC Report
Fri 10 Feb 2017
ERR1633085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527542
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51300.9724344222829652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38630.7322639713994336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18420.349166511860667No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12030.22803871540085907No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11670.22121461419185579No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11400.2160965382851034No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11260.21344272114826876No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC10940.20737685340693254No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10780.20434391953626443No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10710.2030170109678471No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10050.1905061587513411No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.1848194077438384No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9650.18292382407467084No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT9430.17875354000250215No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9390.17799530653483514No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9350.1772370730671681No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG9080.17211899716041568No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9050.1715503220596654No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9010.17079208859199835No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9000.1706025302250816No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT8960.16984429675741458No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8440.1599872616777432No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8360.15847079474240913No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8350.15828123637549238No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8210.15562741923865778No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7850.14880331802965452No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7610.14425391722365233No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7580.14368524212290207No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7560.14330612538906853No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7330.13894628294998315No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7260.13761937438156582No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7140.13534467397856473No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT7040.13344909030939717No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6880.13041615643872906No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6870.1302265980718123No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG6820.1292788062372285No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG6810.12908924787031173No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6640.12586675563272687No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6590.12491896379814309No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6520.12359205522972577No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6440.12207558829439172No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6400.1213173548267247No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6340.12018000462522414No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6240.1182844209560566No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6050.11468281198463819No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5920.11221855321472034No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5870.11127076138013656No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5810.11013341117863601No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5660.10729003567488465No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5640.10691091894105113No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5610.10634224384030086No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5540.10501533527188357No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5530.1048257769049668No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5470.10368842670346627No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5460.1034988683365495No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC5420.10274063486888248No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.10217195976813222No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5390.10217195976813222No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5360.10160328466738194No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5310.10065549283279815No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA5280.10008681773204789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.030.1787721
CCATAGC250.00549368229.5999989
AGCCTCG250.00549368229.59999810
GTGACAT250.00549368229.59999816
TCCGGGT250.00549368229.59999813
CGCCAGG405.9341306E-527.7514
ACTAGTG509.078918E-625.8999988
GGTAGTT509.078918E-625.8999986
AGAGGTG2150.024.95348720
TAGACAT451.3218864E-424.66666820
TATTGCG451.3218864E-424.6666689
TTGTTAC601.3351146E-624.66666815
CTTAGGA952.8012437E-1023.3684222
GTATCAA25350.023.2071021
TACATAC400.001929809723.1253
GAATTAG400.001929809723.1251
GCTAGAG2250.023.02222317
GTAAACA2250.023.02222336
CGTTATT652.6782782E-622.76923410
CGCCGCC1950.022.76923233
Produced by FastQC (version 0.11.5)